A5101843801- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Sperm and Testicular Function
- Circular RNAs in diseases
- Ovarian function and disorders
- Cancer-related molecular mechanisms research
- Sirtuins and Resveratrol in Medicine
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Retinoids in leukemia and cellular processes
- Pregnancy and preeclampsia studies
- interferon and immune responses
- Birth, Development, and Health
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Chemokine receptors and signaling
- Extracellular vesicles in disease
- Reproductive Health and Technologies
- Nutritional Studies and Diet
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Circadian rhythm and melatonin
First Affiliated Hospital of Zhengzhou University
2016-2025
China Pharmaceutical University
2024
Air Force Medical University
2024
Xijing Hospital
2024
Hong Kong Polytechnic University
2024
Bengbu Medical College
2014-2024
Chongqing University
2022-2023
Beijing Tongren Hospital
2023
Capital Medical University
2023
Guangdong Academy of Medical Sciences
2021-2022
Curcumin (Cur) has been widely used in medicine, due to its antibacterial, anti-inflammatory, antioxidant, and antitumor effects. However, clinic application is limited by instability poor solubility. In the present wok, curcumin was loaded into solid lipid nanoparticles (SLNs), order improve therapeutic efficacy for breast cancer. The results measured using transmission electron microscopy (TEM) indicated that Cur-SLNs have a well-defined spherical shape; size about 40 nm with negative...
Background MicroRNAs (miRNAs) are the class of small endogenous RNAs that play an important regulatory role in cells by negatively affecting gene expression at transcriptional and post-transcriptional levels. There have been extensive studies aiming to discover miRNAs analyze their functions from a variety species. However, there no published miRNA profiles human testis using next generation sequencing (NGS) technology. Results We employed Solexa technology profile normal testis. Total 770...
In this study, resveratrol-loaded solid lipid nanoparticles (Res-SLNs) were successfully designed to treat MDA-MB-231 cells. The Res-SLNs prepared using emulsification and low-temperature solidification method. spherical, with small size, negative charge, narrow size distribution. Compared free resveratrol, the displayed a superior ability in inhibiting proliferation of addition, exhibited much stronger inhibitory effects on invasion migration Western blot analysis revealed that could...
Abstract MicroRNAs (miRNAs) have been identified as major post-transcriptional regulators of the initiation and progression human cancers, including breast cancer. However, detail role miR-451 has not fully elucidated in In this study, we aimed to investigate biological molecular mechanisms drug resistance cancer cell lines xenograft model. We show that is decreased specimens paclitaxel-resistant (PR) cells. Ectopic expression could inhibit migration invasion, promoted apoptosis, induced...
// Qingling Yang 1,* , Yangyang Wang 2,* Xiaohui Lu Zunlan Zhao 2 Lihua Zhu Sulian Chen 1 Qiong Wu 3 Changjie and Zhiwei 4 Department of Biochemistry Molecular Biology, Bengbu Medical College, Anhui, China Clinical Testing Diagnose Experimental Center Oncology, First Affiliated Hospital Bengbu, Cyrus Tang Hematology Center, Jiangsu Institute Hematology, Collaborative Innovation Soochow University, Suzhou, Jiangsu, * These authors contributed equally to this work Correspondence: Chen, email:...
OBJECTIVE Whether and how dietary protein intake is linked to type 2 diabetes (T2D) remains unclear. The aim of this study was investigate the associations with development T2D potential mediating roles biomarkers. RESEARCH DESIGN AND METHODS We included 108,681 postmenopausal women without at baseline from Women’s Health Initiative (WHI) (primary cohort) 34,616 adults U.K. Biobank (UKB) (replication cohort). Cox proportional hazard models were used for estimation protein-T2D associations....
Abstract The ovary ages earlier than most other tissues, yet the underlying mechanisms remain elusive. Here a comprehensive analysis of transcriptomic landscapes in different organs young and middle-aged mice revealed that ovaries showed expression age-associated genes, identifying increased NADase CD38 decreased NAD + levels mice. Bulk single-cell RNA sequencing deletion mitigated ovarian aging, preserving fertility follicle reserve aged by countering age-related gene changes intercellular...
Recent advances highlight the pivotal role of nicotinamide adenine dinucleotide (NAD+ ) in ovarian aging. However, roles de novo NAD+ biosynthesis on aging are still unknown. Here, we found that genetic ablation Ido1 (indoleamine-2,3-dioxygenase 1) or Qprt (Quinolinate phosphoribosyl transferase), two critical genes biosynthesis, resulted decreased levels middle-aged mice, leading to subfertility, irregular estrous cycles, reduced reserve, and accelerated Moreover, observed impaired oocyte...
ABSTRACT Recent studies have shown that disruptions in the nicotinamide adenine dinucleotide (NAD + ) de novo synthesis pathway accelerate ovarian aging, yet its role spermatogenesis remains largely unknown. In this study, we investigated impact of NAD on by generating Qprt ‐deficient mice using CRISPR‐Cas9 to target quinolinate phosphoribosyl transferase ( ), a key enzyme predominantly expressed spermatocytes. Our results revealed deletion did not affect levels or testes 3‐month‐old mice....
What is the relationship between telomere length in sperm and early embryonic development vitro fertilization (IVF)? Sperm (STL) positively associated with embryo quality IVF. Previous studies have shown that STL differs among human males. In order to determine associations STL, laboratory parameters clinical pregnancy IVF, 418 couples were recruited from August 2013 2014. We collected semen samples used quantitative PCR technique detect mean for each patient. These data compared IVF...
The quality of postovulatory metaphase II oocytes undergoes a time-dependent deterioration as result the aging process. Melatonin is considered to be an anti-aging agent. However, underlying mechanisms how melatonin improves aged remain largely unclear. In this study, by using mouse model, we found that there were elevated reactive oxygen species levels and impaired mitochondrial function demonstrated reduced membrane potential increased aggregation in 24 h, accompanied number meiotic...
Abstract Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach more effective. The aim of the present study was to compare effects these two methods on sperm telomere length (STL). A total 150 normozoospermic subjects were recruited. STL, DNA fragmentation index (DFI), reactive oxygen species (ROS) content progressive...
Abstract Polycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine disorder characterized by ovulation dysfunction, androgen excess, ovarian polycystic changes, insulin resistance, and infertility. Although underlying mechanisms for PCOS are still unknown, inflammation mitochondrial dysfunction in granulosa cells (GCs) of patients have been reported. Here, we found that Nicotinamide Adenine Dinucleotide (NAD+) levels GCs was significantly decreased when compared with controls....
Multidrug resistance (MDR) is a serious problem during cancer therapy. The purpose of the present study was to formulate D-α-Tocopheryl polyethylene glycol 1000 succinate-resveratrol-solid lipid nanoparticles (TPGS-Res-SLNs) improve its therapeutic efficacy against breast cancer. In this study, solvent injection method used prepare TPGS-Res-SLNs. It found that TPGS-Res-SLNs exhibited zeta potential and drug-loading −25.6 ± 1.3 mV 32.4 2.6%, respectively. Therefore, it evident can increase...
Polycystic ovary syndrome (PCOS) is a complex endocrine metabolic disorder that affects 5–10% of women reproductive age. The endometrium with PCOS has altered immune cells resulting in chronic low-grade inflammation, which attribute to recurrent implantation failure (RIF). In this study, we obtained three and RIF datasets respectively from the Gene Expression Omnibus (GEO) database. By analyzing differentially expressed genes (DEGs) module using weighted gene co-expression networks (WGCNA),...
// Qiong Wu 1,* , Rui Wang Qingling Yang 2 Xin Hou 1 Sulian Chen Yueyue Changjie Yan Lucio Miele 4 Fazlul H Sarkar 5 Yuqing 3 Zhiwei 6,7 Department of Medical Oncology, First Affiliated Hospital Bengbu College, Bengbu, Anhui, China. Biochemistry and Molecular Biology, Respiration, University Mississippi Cancer Institute, Jackson, MS, USA Pathology Karmanos Wayne State University, Detroit, MI 6 Pathology, Beth Israel Deaconess Center, Harvard School, MA, 7 The Cyrus Tang Hematology Jiangsu...
// Rui Wang 1 , Yumei Li Yueyue Hou Qingling Yang 2 Sulian Chen Xi 3 Zishu Yan Changjie Zhiwei 4 Qiong Wu Department of Medical Oncology, First Affiliated Hospital Bengbu College, Bengbu, Anhui, China Biochemistry and Molecular Biology, The 117th PLA, Hangzhou, Cyrus Tang Hematology Center Collaborative Innovation Hematology, Jiangsu Institute the Hospital, Soochow University, Suzhou, Correspondence to: Wu, e-mail: qiongwu68@outlook.com Wang, zwang6@bidmc.harvard.edu Keywords: Hepatocellular...
Breast cancer (BC) is the most prevalent malignant in world, leading cause of cancer-related death female. Recently, there accumulating evidence that long noncoding RNAs (lncRNAs) might as an important role progression BC. (epithelial-mesenchymal transition (EMT) considered to play a vital tumor cells migration and invasion. Nevertheless, entire biological mechanisms functions lncRNAs migration, invasion, EMT remain uncertain. In present research, we observed expression lncRNA AC073284.4 was...
Abstract Breast cancer (BC) is the leading cause of cancer‐related death in women worldwide and one most prevalent malignancy. In recent years, increasing evidence had illuminated that long noncoding RNAs (lncRNAs) serve as critical factors multiple tumor progression, including BC. Emerging references indicated lncRNA H19 acts significant roles progression epithelial‐mesenchymal transition (EMT). However, underlying molecular mechanisms biological BC invasion, metastasis EMT are still...