A5100680882- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Endoplasmic Reticulum Stress and Disease
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Reproductive Health and Technologies
- Sirtuins and Resveratrol in Medicine
- Advanced biosensing and bioanalysis techniques
- Cancer Mechanisms and Therapy
- Circadian rhythm and melatonin
- Reproductive System and Pregnancy
- Plant Disease Management Techniques
- Plant Gene Expression Analysis
- Sulfur Compounds in Biology
- Plant Molecular Biology Research
- Extracellular vesicles in disease
- Antibiotic Resistance in Bacteria
- Plant tissue culture and regeneration
- Light effects on plants
- Plant Physiology and Cultivation Studies
- CRISPR and Genetic Engineering
- PI3K/AKT/mTOR signaling in cancer
First Affiliated Hospital of Zhengzhou University
2021-2024
Yunnan University of Traditional Chinese Medicine
2023-2024
Sichuan Agricultural University
2024
First Affiliated Hospital of Jiangxi Medical College
2024
Nanchang University
2024
Affiliated Hospital of Nantong University
2020-2024
Hunan University
2024
Jiangsu University of Science and Technology
2022-2024
Nantong University
2020-2024
E-Da Hospital
2022-2024
Dysregulated microRNAs (miRNAs) are involved in carcinoma progression, metastasis, and poor prognosis. We demonstrated that nasopharyngeal (NPC), transactivated MIR106A-5p promotes a malignant phenotype by functioning as macroautophagy/autophagy suppressor targeting BTG3 (BTG anti-proliferation factor 3) activating autophagy-regulating MAPK signaling. expression was markedly increased NPC cases based on quantitative real-time PCR, miRNA microarray, TCGA database analysis findings. Moreover,...
Abstract The ovary ages earlier than most other tissues, yet the underlying mechanisms remain elusive. Here a comprehensive analysis of transcriptomic landscapes in different organs young and middle-aged mice revealed that ovaries showed expression age-associated genes, identifying increased NADase CD38 decreased NAD + levels mice. Bulk single-cell RNA sequencing deletion mitigated ovarian aging, preserving fertility follicle reserve aged by countering age-related gene changes intercellular...
Recent advances highlight the pivotal role of nicotinamide adenine dinucleotide (NAD+ ) in ovarian aging. However, roles de novo NAD+ biosynthesis on aging are still unknown. Here, we found that genetic ablation Ido1 (indoleamine-2,3-dioxygenase 1) or Qprt (Quinolinate phosphoribosyl transferase), two critical genes biosynthesis, resulted decreased levels middle-aged mice, leading to subfertility, irregular estrous cycles, reduced reserve, and accelerated Moreover, observed impaired oocyte...
Polycystic ovary syndrome (PCOS) is a complex endocrine metabolic disorder that affects 5–10% of women reproductive age. The endometrium with PCOS has altered immune cells resulting in chronic low-grade inflammation, which attribute to recurrent implantation failure (RIF). In this study, we obtained three and RIF datasets respectively from the Gene Expression Omnibus (GEO) database. By analyzing differentially expressed genes (DEGs) module using weighted gene co-expression networks (WGCNA),...
Background: Melatonin is an indolic compound mainly secreted by the pineal gland and plays a vital role in regulation of circadian rhythms cancer therapy. However, effects melatonin gallbladder (GBC) related mechanism remain unknown. Methods: In this study, antitumor activity on was explored both vitro vivo. After treatment with different concentrations melatonin, cell viability, migration, invasion cells (NOZ GBC-SD cells) were evaluated CCK-8 assay, wound healing, Transwell assay. Results:...
Abstract Matrix stiffness potently promotes the malignant phenotype in various biological contexts. Therefore, identification of gene expression to participate mechanical force signals transduced into downstream biochemical signaling will contribute substantially advances nasopharyngeal carcinoma (NPC) treatment. In present study, we detected that cortactin (CTTN) played an indispensable role matrix stiffness‐induced cell migration, invasion, and invadopodia formation. Advances cancer...
Nasopharyngeal carcinoma (NPC) is one of the most common malignancies in head and neck. Platinum-based chemotherapy an important treatment for NPC. However, molecular mechanism resistance to platinum drug remains unknown. Endoplasmic reticulum resident protein 44(ERp44), unfolded response (UPR)-induced endoplasmic reticulum(ER) protein, induced during ER stress. This research explored ERp44 strengthening cisplatin NPC.Western blot immunohistochemistry were used investigate expression...
Heat stress induces testicular oxidative stress, impairs spermatogenesis, and increases the risk of male infertility. Recent studies have highlighted antioxidative properties Sestrins family in reducing cellular damage. However, role (Sestrin1, 2, 3) response to heat remains unclear. Here, we found that Sestrin2 3 were highly expressed testis relative Sestrin1. Then, Sestrin2-/- Sestrin3-/- mice generated by CRISPR/Cas9 investigate them on spermatogenesis after stress. Our data showed testes...
A mitochondria-targeting fluorescent probe for ClO<sup>−</sup>was developed and a signal amplifier BSA was utilized to promote the signal.
Abstract Purpose Nasopharyngeal carcinoma (NPC) has characteristics of high invasion and early metastasis. Most NPC patients present with locoregionally advanced illness when first diagnosed. Therefore, it is urgent to discover biomarkers. Fibroblast growth Factor 19 (FGF19) plays a role in various physiological or pathological processes, including cancer. In this research, we discovered the importance FGF19 NPC, clarified its tumour angiogenesis. Methods Western blotting,...
Abstract Background Sestrins have been implicated in regulating aging various organs through multiple pathways. However, their roles ovarian remain unrevealed. Methods Female Sestrin1 −/− , Sestrin2 and Sestrin3 mice were generated using the CRISPR-Cas9 system. Body weights, little sizes, estrous cyclicity, follicle number female observed. ELISA was utilized to measure serum anti-Müllerian hormone (AMH) levels. Real time PCR, western blot, immunofluorescence, Masson trichrome staining...
A programmed DNA-cube as a carrier for silver nanoparticles (Ag NPs) to construct specific hydrogen sulfide (H 2 S) responsive platform NP@DNA-cube) diagnosing colorectal cancer (CRC) was proposed in this study.
Abstract Silicon dioxide nanoparticles (SiO 2 NPs) are widely utilized in biomedicine due to their controllable size and biocompatibility. While previous studies have demonstrated that prenatal exposure SiO NPs can traverse the placental barrier induce neurotoxicity offspring. However, reproductive toxicity remains unclear. Here, it is found led subfertility female offspring, evidenced by decreased ovulation potential, ovarian reserve, litter size. In contrast, male offspring maintained...
Hepatocellular carcinoma (HCC) tumors exhibit high heterogeneity. However, current understanding of tumor cell heterogeneity HCC and the association with prognosis remains very limited. In present study, we collected examined tissue from one patient by single-cell RNA sequencing (scRNA-seq). We identified 5753 cells 16 clusters including hepatocytes/cancer cells, T macrophages, endothelial fibroblasts, NK neutrophils, B cells. six subclusters, a cluster proliferative associated poor...
In this work, a three-dimensional (3D) printed microdevice was designed to fix drop of extractant that applied the enrichment most toxic biogenic amine, histamine, by headspace single-drop microextraction (HS-SDME). Concomitantly, based on hybridization chain reaction histamine aptamer isothermal nucleic acid amplification strategy, new fluorescence sensing method developed realize highly sensitive detection histamine. This is first application 3D-printed HS-SDME process, which, among other...
Abstract Reliable detection of circulating small extracellular vesicles (SEVs) and their miRNA cargo has been needed to develop potential specific non-invasive diagnostic therapeutic marker for cancer metastasis. Here, we detected miR-6750, the precise molecular function which was largely unknown, significantly enriched in serum-SEVs from normal volunteers vs. patients with nasopharyngeal carcinoma (NPC). And determined that miR-6750-SEVs attenuated NPC Subsequently, proven inhibit...
Pseudomonas aeruginosa can cause severe infections in humans. This bacterium often adopts a biofilm lifestyle that is hard to treat. In several previous studies, the PprA-PprB two-component system (TCS), which controls expression of type IVb pili, BapA adhesin, and CupE fimbriae, was shown be involved formation (M. Romero, H. Silistre, L. Lovelock, V. J. Wright, K.-G. Chan, et al., Nucleic Acids Res 46:6823-6840, 2018, https://doi.org/10.1093/nar/gky324; S. de Bentzmann, C. Giraud, Bernard,...
As a chemical extensively used in industrial areas and formed during heating of carbohydrate-rich foods tobacco, acrylamide (ACR) has been demonstrated to exert variety systemic toxic effects including hepatotoxicity nephrotoxicity. In the present study, we investigated effect curcumin, natural polyphenolic compound popular spice known as turmeric, on hepatic renal impairment caused by ACR exposure 40 mg/kg for 4 weeks rats. The administration curcumin at doses 50 100 ACR-intoxicated rats...