This study aimed to explore the effects of endometriosis (EMS)-derived exosomes and miR-301a-3p on polarization macrophages investigate involved molecular mechanism. The were isolated from ectopic endometrial tissues EMS patients normal human serum (NHS). Results transmission electron microscope Nanoparticle Tracking Analysis showed that both EMS-exosomes NHS-exosomes are about 80 nm microvesicles. Exosomal markers CD63 TSG101 abundantly expressed in NHS-exosomes. No negative marker Calnexin...

Polycystic ovary syndrome (PCOS) is a known risk factor for uterine endometrial cancer (UCEC), but its underlying mechanisms remain unclear. MicroRNAs (miRNAs) could provide insights into these and reveal potential therapeutic targets. Differential miRNA expression was analyzed in plasma exosomes from 15 PCOS control samples. Survival analysis assessed the prognostic value of miRNAs UCEC. MiRNA-target gene interaction networks co-expression analyses were used to explore molecular mechanisms....

Hepatocellular carcinoma (HCC) is one of the most frequent cancers in world. Calreticulin(CRT) aberrantly overexpressed many human cancer cells. The function CRT HCC cells remains unclear. We attempted to investigate effects and underlying mechanisms down-regulation on cell growth, apoptosis, cycle progression invasion. To cells, small interfering RNA (siRNA) was used knock down expression SMMC7721 HepG2 examined by Western blot immunofluorescence. Cell proliferation detected CCK-8 assay....

As one of the major alkaloid components in Sophoraflavescensait (kushen), oxymatrine has been used widely across world anti-inflammatory and anti-cancer therapies.However, effect metastasis hepatocellular carcinoma (HCC) related mechanism(s) are still unclear.The present study aimed to investigate anti-metastatic on HCC cells.Oxymatrine could also inhibit protein levels MMP-2/-9 a dose-dependent relationship.Moreover, reduces activity p38 signaling pathway via inhibiting phosphorylation...

Gallbladder cancer (GBC) is characterized by poor prognosis, early metastasis, and high recurrence rates, which seriously threaten human health.The effect of lenvatinib, a widely used drug in anti-hepatocellular carcinoma China, on GBC progress, as well its underlying molecular mechanism, remains unclear.Therefore, the present study investigated lenvatinib GBC-SD NOZ cells mechanisms.A series experiments, including cell proliferation, clone formation, wound healing, migration invasion...

Hepatocellular carcinoma (HCC) tumors exhibit high heterogeneity. However, current understanding of tumor cell heterogeneity HCC and the association with prognosis remains very limited. In present study, we collected examined tissue from one patient by single-cell RNA sequencing (scRNA-seq). We identified 5753 cells 16 clusters including hepatocytes/cancer cells, T macrophages, endothelial fibroblasts, NK neutrophils, B cells. six subclusters, a cluster proliferative associated poor...

Recent studies suggested that calreticulin (CRT) has an important role in the progression of various types cancer. Our previous study CRT was upregulated and acted as oncogene hepatocellular carcinoma. However, gallbladder cancer (GBC) remains unclear. The expression level GBC tissues comparison with adjacent non-tumor chronic cholecystitis tissues. Moreover, found to be correlated tumor size. Knockdown inhibited cell proliferation, induced apoptosis, arrested cycle resulted decreased...

To determine which genes are important in placenta by network analysis.Placenta expressing were screened from RNA-Seq data. Protein-protein interaction data downloaded STRING (v11.0) database. Google PageRank (PR) algorithm was used to identify placental protein network. Six disease-related datasets NCBI GEO database, and the differential expression of 99 identified.We calculated PR for each gene defined top with high as genes. GAPDH has highest PR. The had different pattern cell types. FN1...

Objective To retrospectively compare the short-term benefits of robotic surgery and laparoscopic in perioperative period minimally invasive pancreatoduodenectomy (MIPD). Methods This retrospective analysis evaluated patients who underwent (LPD) or (RPD) from March 2018 to January 2022 First Affiliated Hospital Zhengzhou University (Zhengzhou, China). Perioperative data, including operating time, complications, morbidity mortality, estimated blood loss (EBL), postoperative length stay, were...

At present, in the field of commercial banks, customer relationship management (CRM) is a focus customer-centric banks.Who can accurately use data mining technology to bank information classification, and processing will success.And then customers should be segmented order timely make adjustments improvements adapt market changes, seek more business interests.To this end, paper analyses role mining, as well their current development.Through analysis K-means algorithm large number summary...

Objective To study the expression and clinical significance of hypoxia-induced factor-1α (HIF-1α ) in gallbladder cancer tissues, role mechanism HIF-1α metformin-suppressed metastasis carcinoma GBC-SD cells. Methods 24 specimens tissues 5 chronic cholecystitis were collected from First Affiliated Hospital Zhengzhou University between June 2016 February 2017. Immunohistochemistry qPCR used to detect adjacent non-cancer cholecystitis, was analyzed. The model induced by hypoxia; wound...

In the promotion of e-commerce, logistics industry gradually develops to information and network direction, therefore, in e-commerce environment, management can not completely copy model under traditional However, mode needs be analyzed adjusted again.This paper first describes characteristics then analyzes shortcomings this finally puts forward suggestion innovation mode.

Pancreatic tuberculosis (PTB) is a rare, chronic, specific and infectious disease which generally secondary to at the common sites of pancreas, it has high misdiagnosis rate due hidden onset non-specific symptoms PTB. A patient with PTB was admitted First Affiliated Hospital Zhengzhou University in June 2014. Before operation, space-occupying lesions head pancreas were detected by preoperative imaging examination, regarded as pancreatic cancer. Intraoperative exploration showed cystic duct...

Abstract Malignant fibrous histiocytoma (MFH) is one of most common types soft-tissue sarcoma. However, it accounts for less than 1% all human cancer types. In addition, primary MFH the pancreas very rare. Herein, we report a 37-year-old man who presented with pancreatic mass and did not show any specific symptoms. Pre-contrast computed tomography (CT) showed heterogeneous, liquid-filled necrotic in uncinate lobe pancreas. Contrast-enhanced CT mild enhancement solid components...

Objective To investigate the expression of poly adenosine di-phosphate (ADP)-ribose polymerase-1 (PARP-1) in hepatocellular carcinoma (HCC) tissues and its relationship with epithelial-mesenchymal transition (EMT). Methods Eigthy-two cases HCC undergoing elective surgical resection were selected. The levels PARP-1, E-cadherin, Vimentin Snail proteins paracancerous detected by immunohistochemical staining. genes using real-time fluorescent quantitative polymerase chain reaction...

Objective To study the effect of gallbladder surgery on immune function and gastrointestinal guinea pigs with gallstones model, provide reference for individualized treatment gallstones. Methods Thirty model were randomly divided into group cholecystectomy group, given respectively. Four weeks after surgery, animals killed. The function, small intestine colon propulsion villus height, width, mucosal thickness compared between two groups. Results In cells CD3+ T cells, CD40+ B...

Objective To explore the effect of metformin on apoptosis thyroid cancer TPC-1 cells and its mechanism. Methods The proliferation treated with different concentrations were detected using cell count kit-8 (CCK-8) assay flow cytometry respectively. Western blot Real-time PCR used to detect expression endoplasmic reticulum chaperone immunoglobulin heavy chain binding protein (Bip) stress associated CCAAT/enhancer-binding homologous (CHOP) cysteinyl aspartate specific proteinase...