A5030545077- Single-cell and spatial transcriptomics
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Congenital heart defects research
- Adipokines, Inflammation, and Metabolic Diseases
- Sinusitis and nasal conditions
- Memory and Neural Mechanisms
- Regulation of Appetite and Obesity
- Neuroinflammation and Neurodegeneration Mechanisms
- Tracheal and airway disorders
- Neurogenesis and neuroplasticity mechanisms
- Erythrocyte Function and Pathophysiology
- Nasal Surgery and Airway Studies
- Lipid metabolism and biosynthesis
SUNY Downstate Health Sciences University
2022-2024
State University of New York
2022-2024
Cell membrane phosphatidylcholine (PC) composition is regulated by lysophosphatidylcholine acyltransferase (LPCAT); changes in PC saturation are implicated metabolic disorders. Here, we identified LPCAT3 as the major isoform of LPCAT adipose tissue and created adipocyte-specific Lpcat3–knockout mice to study lipid metabolism. Transcriptome sequencing plasma adipokine profiling were used investigate how regulates insulin signaling. deficiency reduced polyunsaturated PCs adipocyte membranes,...
The study of neurodevelopmental molecular mechanisms in schizophrenia requires the development adequate biological models such as patient-derived cells and their derivatives. We previously utilized cell lines with neural progenitor properties (CNON) derived from superior or middle turbinates patients control groups to schizophrenia-specific gene expression. In this study, we analyzed single-cell RNA seq data two CNON (one an individual (SCZ) other a group) biopsy samples turbinate (MT) (also...
Abstract Objective Single‐cell RNA‐sequencing of middle turbinate mucosa was performed to create the first single‐cell transcriptome catalog this part human body. Study Design Basic science research. Setting Single center, tertiary care center. Methods Samples were obtained from head a healthy volunteer. After specimen prepared per lab protocol, cells dissociated, resuspended, and counted. libraries then according 10x Genomics protocol sequenced using NovaSeq 6000 (Illumina). Sequencing data...
Abstract Importance Elucidation of the cellular makeup middle turbinate provides a foundation for future studies pathogenesis sinonasal disease. Neural progenitors and pluripotent basal cells found in mucosa potentially can be used to develop models study brain disorders or regenerative medicine substitute neuronal tissues. Objective Single cell RNA-sequencing (scRNA-seq) was performed create first single transcriptome catalog this part human body. Design Samples were obtained from head...
ABSTRACT The rodent hippocampus is a spatially organized neuronal network that supports the formation of spatial and episodic memories. We conducted bulk RNA sequencing transcriptomics experiments to measure gene expression changes in dorsal following recall active place avoidance (APA) memory. Through sequencing, we examined memory across functionally distinct subregions hippocampus. found induced differentially expressed genes (DEGs) CA1 CA3 hippocampal were enriched with involved synaptic...
The rodent hippocampus is a spatially organized neuronal network that supports the formation of spatial and episodic memories. We conducted bulk RNA sequencing transcriptomics experiments to measure gene expression changes in dorsal following recall active place avoidance (APA) memory. Through sequencing, we examined memory across functionally distinct subregions hippocampus. found induced differentially expressed genes (DEGs) CA1 CA3 hippocampal were enriched with involved synaptic...
Study of the neurodevelopmental molecular mechanisms schizophrenia requires development adequate biological models such as patient-derived cells and their derivatives. We previously used cell lines with neural progenitor properties (CNON) derived from superior or middle turbinates patients control groups to study gene expression specific schizophrenia. In this study, we compared single cell-RNA seq data two CNON lines, one an individual (SCZ) other a group, biopsy samples turbinate (MT),...
<p>Cell membrane phosphatidylcholine composition is regulated by lysophosphatidylcholine acyltransferase (LPCAT); changes in saturation are implicated metabolic disorders. Here, we identified LPCAT3 as the major isoform of LPCAT adipose tissues and created adipocyte-specific <i>Lpcat3</i>-knockout mice to study tissue lipid metabolism. Transcriptome sequencing plasma adipokine profiling were used investigate how regulates insulin signaling. deficiency reduced...
<p>Cell membrane phosphatidylcholine composition is regulated by lysophosphatidylcholine acyltransferase (LPCAT); changes in saturation are implicated metabolic disorders. Here, we identified LPCAT3 as the major isoform of LPCAT adipose tissues and created adipocyte-specific <i>Lpcat3</i>-knockout mice to study tissue lipid metabolism. Transcriptome sequencing plasma adipokine profiling were used investigate how regulates insulin signaling. deficiency reduced...