A5057919229- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Crustacean biology and ecology
- Aquaculture Nutrition and Growth
- Marine Bivalve and Aquaculture Studies
- Invertebrate Immune Response Mechanisms
- Insect and Arachnid Ecology and Behavior
- Heat shock proteins research
- Genetics, Aging, and Longevity in Model Organisms
- Ocean Acidification Effects and Responses
- Mass Spectrometry Techniques and Applications
- Fish Ecology and Management Studies
- Advanced Proteomics Techniques and Applications
- Marine Biology and Environmental Chemistry
- Chemical Synthesis and Analysis
- Genetic diversity and population structure
- Amino Acid Enzymes and Metabolism
- Photoreceptor and optogenetics research
- Meat and Animal Product Quality
- Parasite Biology and Host Interactions
- Marine Biology and Ecology Research
- Protein Hydrolysis and Bioactive Peptides
- Identification and Quantification in Food
- Cephalopods and Marine Biology
- Neuroendocrine regulation and behavior
Station Biologique de Roscoff
2013-2024
Sorbonne Université
2008-2023
Centre National de la Recherche Scientifique
2011-2023
Adaptation et Diversité en Milieu Marin
2010-2021
Université Paris Cité
2008-2013
Bioénergétique et Ingénierie des Protéines
2000-2008
Biogen (Switzerland)
2006-2008
Unité de Glycobiologie Structurale et Fonctionnelle
2006
École Normale Supérieure - PSL
1991-2002
ESPCI Paris
1998
Background The Antarctic krill Euphausia superba is a keystone species in the food chain. Not only it significant grazer of phytoplankton, but also major item for charismatic megafauna such as whales and seals an important Southern Ocean fisheries crop. Ecological data suggest that this being affected by climate change will have considerable consequences balance ecosystem. Hence, understanding how organism functions priority area provide fundamental life history studies, energy budget...
Crustacean Hyperglycemic Hormone (CHH) family peptides are neurohormones known to regulate several important functions in decapod crustaceans such as ionic and energetic metabolism, molting reproduction. The structural conservation of these peptides, together with the variety they display, led us investigate their evolutionary history. CHH exist insects (Ion Transport Peptides) may be present all ecdysozoans well. In order extend study entire family, were thus searched taxa outside decapods,...
Background The Ice krill, Euphausia crystallorophias is one of the species at base Southern Ocean food chain. Given their significant contribution to biomass Ocean, it vitally important gain a better understanding physiology and, in particular, anticipate responses climate change effects warming seas around Antarctica. Methodology/Principal Findings Illumina sequencing was used produce transcriptome ice krill. Analysis assembled contigs via two different methods, produced 36 new...
Background A comparative thermal tolerance study was undertaken on two sister species of Euphausiids (Antarctic krills) Euphausia superba and crystallorophias. Both are essential components the Southern Ocean ecosystem, but occupy distinct environmental geographical locations with slightly different temperature regimes. They therefore provide a useful model system for investigation adaptations to tolerance. Methodology/Principal Finding Initial CTmax studies showed that E. more...
The European flat oyster (
Modification of the chirality a single amino acid residue within peptide chain appears to be novel additional mechanism leading structural and functional diversification eukaryotic bioactive peptides. This phenomenon has been studied at cellular level in neuroendocrine organ which elaborates mixture diastereoisomers 72-residue neuropeptide, crustacean hyperglycemic hormone. For first time, isomerization shown occur perikarya fully specialized neurosecretory cells, as late step maturation...
The combination of two sensitive and powerful analytical techniques on the same biological sample was examined: (i) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS),which gives informative peptide profiling complex samples such as organs or cells; (ii) immunological tools enzyme-linked immunosorbent assay (ELISA) immunocytochemistry to probe for specific peptides in extracts cells. cellular expression precursors hyperglycemic hormone (cHH) analyzed...
Crustacean hyperglycemic hormone (CHH) and vitellogenesis-inhibiting (VIH), produced by the X organ-sinus gland neurosecretory complex, belong to a peptide group referred as CHH family, which is widely distributed in arthropods. In this study, genetic variants post-translationally modified isoforms of VIH were characterized European lobster Homarus gammarus. With use RP-HPLC ELISA with specific antibodies that discriminate between stereoisomers VIH, two groups CHH-immunoreactive peaks from...
The annelid Paralvinella grasslei is a deep-sea vent endemic species that colonizes the wall of active chimneys. We report here first data on its thermal biology based in vivo experiments pressurized aquaria. Our results demonstrate P. survives 30 min exposure at degrees C, and suggest upper limit this slightly above temperature. signs stress were noticed such as significant increase animal's activity expression HSP70 proteins. A preliminary investigation kinetics protein surprisingly showed...
The Arctic and the Antarctic Peninsula are currently experiencing some of most rapid rates ocean warming on planet. This raises question how initial adaptation to extreme cold temperatures was put in place whether or not directional selection has led loss genetic variation at key adaptive systems, thus polar species' (re)adaptability higher temperatures. In Southern Ocean, krill represents abundant fauna is a critical member base food web. To better understand role shaping current patterns...
Abstract A subgroup of neurons in the classical X‐organ‐sinus gland neuroendocrine system crayfish ( Orconectes limosus ) eyestalk produces two chiral forms crustacean hyperglycemic hormone (CHH) different types neurons: CHH ∼22 cells and D‐Phe 3 eight cells. Previous reports have demonstrated that release from sinus is inhibited by enkephalins. Here, we addressed questions 1) whether this inhibition affects one or both neurons, 2) where site enkephalinergic control and/or is, 3) inhibitory...
In order to functionally characterize the metabolic roles of crustacean hyperglycemic hormone (CHH), gene expression CHH in crayfish (Procambarus clarkii) was knocked down by vivo injection double-stranded RNA (dsRNA), followed metabolomic analysis 2 target tissues (the muscle and hepatopancreas) using nuclear magnetic resonance spectroscopy. Compared levels untreated saline-injected (SAI) animals, transcript, but not those molt-inhibiting (a CHH-family peptide), eyestalk ganglia...
Summary Ecdysteroids secreted in vitro by Y-organs of the white shrimp Penaeus vannamei were quantified at different stages molt cycle enzyme immunoassay (EIA). The production was maximal during first hour incubation, and highest values obtained with from animals D1′″. D2-D3. Ecdysteroid increased parallel rise hemolymph ecdysteroid levels, which peaked late premolt stage A preliminary characterization showed that 20-hydroxyecdysone major circulating ecdysteroid. present incubation median...