A5011206996- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Luminescence Properties of Advanced Materials
- Radiation Detection and Scintillator Technologies
- Perovskite Materials and Applications
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
Institut Fresnel
2021-2024
Centrale Marseille
2021-2024
Centre National de la Recherche Scientifique
2021-2024
Aix-Marseille Université
2024
University of Milano-Bicocca
2020
Abstract In the quest to decipher chain of life from molecules cells, biological and biophysical questions being asked increasingly demand techniques that are capable identifying specific biomolecules in their native environment, can measure biomolecular interactions quantitatively, at smallest possible scale space time, without perturbing system under observation. The interaction light with offers a wealth phenomena tools be exploited drive this progress. This Roadmap is written...
Coherent non-linear wide-field imaging reduces phototoxicity compared to focused approaches and holds the potential for highest possible image acquisition rates, but misses optical sectioning. Here, we reintroduce sectioning using fast changing speckle illuminations.
In the last decades, nonlinear optical microscopy techniques, such as two-Photon Excited Fluorescence (2PEF), Sum Frequency Generation (SFG), and Hyperspectral Coherent Anti-Stokes Raman Scattering (H-CARS) microscopy, emerged powerful tools to image biological samples in a label-free, fast, non-destructive way. Traditionally, these techniques are performed with single point-scanning acquisition schemes featuring limited field of view often leading sample damage when high laser power is used...
We revisit wide field CARS imaging using speckle illuminations. In the proposed scheme we break coherence fast varying pump illuminations while keeping static Stokes speckle. Acquiring a large number of images enables dynamic illumination (DSI) and random microscopy (RIM). show that first one quasi-confocal axial sectioning (1/z) second one, through post-processing, can retrieve superior image contrast, noise level spatial resolution as an important step towards robust nonlinear...