A5086941145- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Digital Holography and Microscopy
- Spectroscopy and Chemometric Analyses
- Gold and Silver Nanoparticles Synthesis and Applications
Cardiff University
2021-2024
We report an experimental scheme for stimulated Raman scattering (SRS) microscopy with excitation in the visible spectral region. This allows electronically preresonant (epr) SRS of a broad range chromophores sensitivities as low 1 μM. Our experiment is based on two synchronously near-infrared pumped optical parametric oscillators (OPO). One outputs modulated at fourth repetition rate novel broadband electro-optical modulator. Using combination focusing and tuning OPO, we show recording...
Abstract Coherent anti‐Stokes Raman scattering microscopy probing two independently selectable vibrational frequencies across the spectrum is demonstrated. A one‐box femtosecond laser source provides three synchronised 100–200 fs pulses of 77 MHz repetition rate, one at a fixed wavelength 1025 nm from an Yb oscillator and tuneable ones, over 680–960 960–1300 nm, respectively, optically parametric oscillators. Combined with spectral focusing, allows addressing fingerprint, cell silent C–H...
Abstract In the quest to decipher chain of life from molecules cells, biological and biophysical questions being asked increasingly demand techniques that are capable identifying specific biomolecules in their native environment, can measure biomolecular interactions quantitatively, at smallest possible scale space time, without perturbing system under observation. The interaction light with offers a wealth phenomena tools be exploited drive this progress. This Roadmap is written...
We demonstrate coherent anti-Stokes Raman scattering (CARS) microscopy with 1089 foci, enabled by a high repetition rate amplified oscillator and optical parametric amplifier. employ camera as multichannel detector to acquire separate the signals from rather than using image itself. This allows retain insensitivity of imaging sample afforded non-linear excitation point-spread function, which is hallmark point-scanning techniques. show frame rates 0.3Hz for megapixel CARS image, limited used....
We demonstrate coherent anti-Stokes Raman scattering (CARS) microscopy with 1089 foci, enabled by a high repetition rate amplified oscillator and an optical parametric amplifier. employ camera as multichannel detector to acquire separate the signals from rather than using image itself. This allows us retain insensitivity of imaging afforded non-linear excitation point-spread function, which is hallmark point-scanning techniques. show frame rates 0.3 Hz for megapixel CARS image, limited used....
Coherent non-linear wide-field imaging reduces phototoxicity compared to focused approaches and holds the potential for highest possible image acquisition rates, but misses optical sectioning. Here, we reintroduce sectioning using fast changing speckle illuminations.
We revisit wide field CARS imaging using speckle illuminations. In the proposed scheme we break coherence fast varying pump illuminations while keeping static Stokes speckle. Acquiring a large number of images enables dynamic illumination (DSI) and random microscopy (RIM). show that first one quasi-confocal axial sectioning (1/z) second one, through post-processing, can retrieve superior image contrast, noise level spatial resolution as an important step towards robust nonlinear...