A5048197206- Spectroscopy Techniques in Biomedical and Chemical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy and Chemometric Analyses
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy and Quantum Chemical Studies
- Digital Holography and Microscopy
- Photoreceptor and optogenetics research
- Toxic Organic Pollutants Impact
- Integrated Circuits and Semiconductor Failure Analysis
- Thermography and Photoacoustic Techniques
- Bacillus and Francisella bacterial research
University of Konstanz
2020-2025
ABSTRACT Coherent anti‐Stokes Raman scattering (CARS) and stimulated (SRS) microscopy are key techniques for label‐free vibrational imaging. However, their application is often limited by poor sensitivities. Recent advancements have shown that similar to spontaneous resonance spectroscopy, SRS sensitivity can be greatly enhanced under electronic preresonance conditions. While enhancement of CARS signals in spectroscopy was reported the 1970s, its remains less explored. This study presents...
Double modulation of the excitation light gives quantitative insight into signal contributions in pre-resonance SRS and SREF spectroscopy.
We report an experimental scheme for stimulated Raman scattering (SRS) microscopy with excitation in the visible spectral region. This allows electronically preresonant (epr) SRS of a broad range chromophores sensitivities as low 1 μM. Our experiment is based on two synchronously near-infrared pumped optical parametric oscillators (OPO). One outputs modulated at fourth repetition rate novel broadband electro-optical modulator. Using combination focusing and tuning OPO, we show recording...
Abstract In the quest to decipher chain of life from molecules cells, biological and biophysical questions being asked increasingly demand techniques that are capable identifying specific biomolecules in their native environment, can measure biomolecular interactions quantitatively, at smallest possible scale space time, without perturbing system under observation. The interaction light with offers a wealth phenomena tools be exploited drive this progress. This Roadmap is written...
Stimulated Raman Scattering microscopy is an important imaging technique. Its broader application, however, hampered by its comparatively low sensitivity. Using organic fluorophores, it has recently been demonstrated that, similar to spontaneous microscopy, the sensitivity of stimulated increased orders magnitudes if electronic preresonances are exploited. In this Article, we show that approach also works with quantum yield chromophores. We investigate relevant photophysics and discuss...