A5102872310- Platelet Disorders and Treatments
- Gastrointestinal motility and disorders
- Congenital gastrointestinal and neural anomalies
- Ion Channels and Receptors
- Neuroscience and Neural Engineering
- Ultrasound and Hyperthermia Applications
- Protease and Inhibitor Mechanisms
- Mast cells and histamine
- Influenza Virus Research Studies
- Anaerobic Digestion and Biogas Production
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neural dynamics and brain function
- Proteoglycans and glycosaminoglycans research
- Additive Manufacturing and 3D Printing Technologies
- Protein Kinase Regulation and GTPase Signaling
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood properties and coagulation
- Neuroscience and Neuropharmacology Research
- Respiratory viral infections research
- Ultrasound Imaging and Elastography
- Lymphoma Diagnosis and Treatment
- Blood disorders and treatments
- Muscle activation and electromyography studies
University of Connecticut
2016-2024
Inserm
1997-2004
Arizona State University
2004
Groupe Hospitalier de l'Institut Catholique de Lille
2004
Institut Pasteur
1997-1998
Three-dimensional (3D) printing offers potential to fabricate high-throughput and low-cost fabrication of microfluidic devices as a promising alternative traditional techniques which enables efficient design iterations in the development stage. In this study, we demonstrate single-step 3D transparent chip using two techniques: stereolithography-based desktop printer two-step an industrial based on polyjet technology. This method, compared conventional relatively expensive materials...
We reveal that VGLUT2-positive enteric neurons (EN), although constituting a small fraction of total EN, are homogeneously expressed in the myenteric ganglia, with slight concentration at intermediate region between colon and rectum. Through anatomic, molecular, functional analyses, we demonstrated VGLUT2-ENs activated indirectly by noxious circumferential colorectal stretch via nicotinic cholinergic transmission, suggesting their participation mechanical visceral nociception.
Ultrasound that is widely used in medical diagnosis has drawn growing interests as a noninvasive means of neuromodulation. Focused pulsed ultrasound (FPUS) effectively modulates neural encoding and transmission the peripheral nervous system (PNS) with unclear mechanism action, which further confounded by contradictory experimental outcomes from recordings compound action potentials (CAP). To address that, we developed novel vitro set up to achieve simultaneous single-unit individual mouse...
Electrode arrays interfacing with peripheral nerves are essential for neuromodulation devices targeting organs to relieve symptoms. To modulate (i.e., single-unit recording and stimulating) individual nerve axons remains a technical challenge. Here, we report an in vitro setup allow simultaneous recordings from multiple mouse sciatic axons. The (∼30 mm) was harvested transferred tissue chamber, the ∼5 mm distal end pulled into adjacent chamber filled paraffin oil. A custom-built multi-wire...
We conducted genetic labeling and optical clearing to visualize extrinsic sensory nerve fibers in whole-mount colorectum, which revealed widespread presence of axons the submucosal layer. Remarkably, submucosa were meandering curvy, contrast other layers generally aligned with basal tissues. Intracolonic TNBS treatment led pronounced changes fiber density curviness, suggesting morphologies as potentially contributing factors sensitization.
Platelets are one source of the group II extracellular form phospholipase A2 (sPLA2) which is involved in amplification local and systemic inflammation. Although sPLA2 protein has been described human platelets, its presence megakaryocytes not yet established. We demonstrated this study that erythroleukaemia (HEL) cell line, megakaryoblastic features, constitutively expresses sPLA2. Using an anti‐rhsPLA2 monoclonal antibody (mAb BA11) dot‐blot detection, we showed HEL cells platelets release...
Summary Recombinant staphylokinase (RSTA) has been shown to offer promise as a thrombolytic agent. In contrast streptokinase (SK), few studies have devoted possible effects of RSTA on platelets. We compared the capacity and SK trigger platelet aggregation modify ADP (2.5 µM) response in platelet-rich plasma (PRP) 25 healthy subjects. Thus, exposure PRP (40 50 µg/ml) induced 6 out However, under same conditions, failed induce all cases (25 subjects). RSTA, (0.4 greatly reduced ADP-induced 12...
The exposure of human platelets to prostaglandin H 2 analogue (PGH2, U46619) induces homologous desensitization and a concomitant adenylate cyclase (AC) sensitization. We demonstrate the involvement phospholipase C (PLC) in this enzyme Pre‐incubation with neomycin, PLC activity inhibitor, prevented AC sensitization but not PGH /thromboxane (Tx)A receptor desensitization. /TxA desensitization, although necessary, is sufficient induce sensitization, since which prevents failed prevent Inositol...
Streptokinase (SK) is one of the plasminogen activators currently used in therapeutics. SK antibodies may appear blood after thrombolytic therapy with or ss-hemolytic streptococci infection. Such both activate platelets and neutralize ability to convert into plasmin. We previously demonstrated that platelet activation induced by combination IgG anti-SK anisoylated plasminogen-SK activator complex (APSAC) mediated Fgamma7RIIal receptor. However, mechanism which APSAC (or SK) transduce an...
Abstract The enteric nervous system (ENS) functions largely independently of the central (CNS). Correspondingly, glutamate, dominant neurotransmitter in CNS and sensory afferents, is not a primary ENS. Only fraction (approximately 2%) myenteric neurons mouse distal colon rectum (colorectum) are positive for vesicular glutamate transporter type 2 (VGLUT2), structure function which remain undetermined. Here, we systematically characterized VGLUT2-positive (VGLUT2-ENs) through sparse labeling...