A5053296362- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Glaucoma and retinal disorders
- Ocular Diseases and Behçet’s Syndrome
- Bacteriophages and microbial interactions
- Infectious Diseases and Tuberculosis
- Ocular Infections and Treatments
- Viral Infections and Outbreaks Research
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Retinal and Optic Conditions
- Retinal Diseases and Treatments
University of Virginia
2024
Royal Devon & Exeter NHS Foundation Trust
2014
The University of Texas Health Science Center at Tyler
1996
Centers for Disease Control and Prevention
1996
Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species organisms, no typing method is available; for others, methods are tedious. We developed a amplifying sequences flanking infrequent restriction sites PCR used the to produce strain-specific electrophoretic patterns from crude lysates. This rapid, sensitive, widely applicable. Identical enzymes, adaptors, primers, conditions were characterize 32...
The detection of Mycobacterium tuberculosis by culture cerebrospinal fluid (CSF) is unacceptably slow. Low numbers organisms and the presence reaction inhibitors may prevent M. PCR. We used immunomagnetic enrichment to accelerate enhance mycobacteria in CSF after demonstrating utility method with pure suspensions. Growth was detected earlier Bactec cultures magnetically recovered than untreated (7 versus 15 days). DNA PCR immunomagnetically enriched sample but not CSF. fingerprintings...
In 1994 a Texas prison containing population of mentally retarded inmates experienced large tuberculosis outbreak. Fifteen cases were identified (8 confirmed by positive cultures for Mycobacterium tuberculosis) and more than 100 became infected. The culture-confirmed patients infected with an identical strain as demonstrated polymerase chain reaction (PCR) based DNA fingerprinting technique. followed standard infection control policies, but these controls inadequate to prevent transmission...