A5032788453- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Nanoparticle-Based Drug Delivery
- Computational Drug Discovery Methods
- Bacterial Genetics and Biotechnology
- Barrier Structure and Function Studies
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Transgenic Plants and Applications
- Biochemical and Structural Characterization
University of Florida
2023-2025
The Ohio State University
2024
Developing potent and selective protease inhibitors remains a grueling, iterative, often unsuccessful endeavor. Although macromolecular can achieve single-enzyme specificity, platforms used for inhibitor discovery are optimized high-affinity binders, requiring extensive downstream biochemical characterization to isolate rare inhibitors. Here, we developed the High-throughput Activity Reprogramming of Proteases (HARP) platform, HARP is yeast-based functional screen that isolates...
Although the rapid development of therapeutic responses to combat SARS-CoV-2 represents a great human achievement, it also demonstrates untapped potential for advanced pandemic preparedness. Cross-species efficacy against multiple coronaviruses by main protease (MPro) inhibitor nirmatrelvir raises question its breadth inhibition and our preparedness future coronaviral threats. Herein, we describe sequence structural analyses 346 unique MPro enzymes from all represented in NCBI Virus...
Abstract The yeast endoplasmic reticulum sequestration and screening (YESS) system is a broadly applicable platform to perform high-throughput biochemical studies of post-translational modification enzymes (PTM-enzymes). This enables researchers profile engineer the activity substrate specificity PTM-enzymes discover inhibitor-resistant enzyme mutants. In this study, we expand capabilities YESS by transferring its functional components integrative plasmids. yields uniform protein expression...
Abstract The yeast endoplasmic reticulum sequestration and screening (YESS) system is a generalizable platform that has become highly useful to investigate post-translational modification enzymes (PTM-enzymes). This enables researchers profile engineer the activity substrate specificity of PTM-enzymes discover inhibitor-resistant enzyme mutants. In this study, we expand capabilities YESS by transferring its functional components integrative plasmids. yields uniform protein expression...