A5009079272- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Inhalation and Respiratory Drug Delivery
- Plant Pathogenic Bacteria Studies
- Cystic Fibrosis Research Advances
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Islamic Finance and Banking Studies
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Microbial infections and disease research
- Zoonotic diseases and public health
- Animal Disease Management and Epidemiology
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Bacterial biofilms and quorum sensing
University of Alberta
2007-2023
Veterinary Medicines Directorate
2010-2011
Animal and Plant Health Agency
2011
National Institute for Nanotechnology
2011
PHAge Search Tool (PHAST) is a web server designed to rapidly and accurately identify, annotate graphically display prophage sequences within bacterial genomes or plasmids. It accepts either raw DNA sequence data partially annotated GenBank formatted performs number of database comparisons as well phage 'cornerstone' feature identification steps locate, features. Relative other tools, PHAST up 40 times faster 15% more sensitive. also able process both Genbank files, provide richly tables on...
To determine the feasibility of formulating and aerosolizing powders containing bacteriophages KS4-M ΦKZ for lung delivery treatment pulmonary Burkholderia cepacia complex Pseudomonas aeruginosa infections.Endotoxin-removed were lyophilized in lactose/lactoferrin 60 : 40 w/w matrix deagglomerated a mixer mill (without beads) to formulate respirable powders. The then aerosolized using an Aerolizer(®) capsule inhaler. Mass median aerodynamic diameter (MMAD) this inhalable aerosol was...
A rapid worldwide increase in the number of human infections caused by extremely antibiotic resistant bacterium Stenotrophomonas maltophilia is prompting alarm. One potential treatment solution to current resistance dilemma "phage therapy", clinical application bacteriophages selectively kill bacteria.
Abstract Background The Burkholderia cepacia complex (BCC) is comprised of at least seventeen Gram-negative species that cause infections in cystic fibrosis patients. Because BCC bacteria are broadly antibiotic resistant, phage therapy currently being investigated as a possible alternative treatment for these infections. purpose our study was to sequence and characterize three novel BCC-specific phages: KS5 (vB_BceM-KS5 or vB_BmuZ-ATCC 17616), KS14 (vB_BceM-KS14) KL3 (vB_BamM-KL3...
Genomic analysis of bacteriophages infecting the Burkholderia cepacia complex (BCC) is an important preliminary step in development a phage therapy protocol for these opportunistic pathogens. The objective this study was to characterize KL1 (vB_BceS_KL1) and AH2 (vB_BceS_AH2), two novel cenocepacia-specific siphoviruses isolated from environmental samples.KL1 exhibit several unique phenotypic similarities: they infect same B. cenocepacia strains, require prolonged incubation at 30°C...
The Burkholderia cepacia complex (BCC) is made up of at least 17 species gram-negative opportunistic bacterial pathogens that cause fatal infections in patients with cystic fibrosis and chronic granulomatous disease. KS9 (vB_BcenS_KS9), one a number temperate phages isolated from BCC species, prophage pyrrocinia LMG 21824. Transmission electron micrographs indicate belongs to the family Siphoviridae exhibits B1 morphotype. 39,896-bp genome, comprised 50 predicted genes, integrates into 3'...
Abstract Background The Burkholderia cepacia complex (BCC) is a versatile group of Gram negative organisms that can be found throughout the environment in sources such as soil, water, and plants. While BCC bacteria involved beneficial interactions with plants, they are also considered opportunistic pathogens, specifically patients cystic fibrosis chronic granulomatous disease. These exhibit resistance to many antibiotics, making conventional treatment often unsuccessful. KS10 was isolated...
Abstract Background As is true for many other antibiotic-resistant Gram-negative pathogens, members of the Burkholderia cepacia complex (BCC) are currently being assessed their susceptibility to phage therapy as an antimicrobial treatment. The objective this study was perform genomic and limited functional characterization novel BCC JG068 (vB_BceP_JG068). Results a podovirus that forms large, clear plaques on cenocepacia K56-2. Host range analysis indicates can infect environmental,...
Burkholderia is an important bacterial genus with a complex taxonomy that contains species of both ecological and pathogenic importance, including nine closely related collectively termed the cepacia (BCC). Unfortunately, 16S rRNA gene analysis has proven to be not sensitive enough discriminate between BCC. Alternative identification strategies such as recA-based PCR followed by restriction fragment length polymorphism analysis, although initially useful, have inaccurate increasing diversity...
ABSTRACT Organic compounds exhibit various levels of toxicity toward living organisms based upon their ability to insert into biological membranes and disrupt normal membrane function. The primary mechanism responsible for organic solvent tolerance in many bacteria is energy-dependent extrusion via efflux pumps. One such bacterial strain, Pseudomonas putida S12, known its high solvents as provided through the SrpABC resistance-nodulation-cell division (RND) family pump. To determine how two...
Bcep22-like phages are a recently described group of podoviruses that infect strains Burkholderia cenocepacia. We have isolated and characterized novel member this named DC1. This podovirus shows many genomic similarities to BcepIL02 Bcep22, but it infects belonging multiple cepacia complex (BCC) species.
Between May 2005 and June 2008, strategically selected isolates of Escherichia coli obtained from clinical submissions to Veterinary Laboratories Agency (VLA) regional laboratories in England Wales were serogrouped examined by PCR for verocytotoxin (VT) production attaching effacing ( eae ) genes, both which are zoonotic determinants. VT‐encoding genes detected 54 (5.3 per cent) the 1022 examined. Only one isolate (0.1 was identified as verocytotoxigenic E (VTEC) O157. Non‐O157 VTECs present...
The Burkholderia cepacia complex (Bcc) is a group of 17 Gram-negative predominantly environmental bacterial species that cause potentially fatal opportunistic infections in cystic fibrosis (CF) patients. Although its prevalence these individuals lower than Staphylococcus aureus and Pseudomonas aeruginosa , the Bcc remains serious problem CF community because pathogenicity, transmissibility, inherent antibiotic resistance organisms. An alternative treatment for currently being developed phage...
Characterization of prophages in sequenced bacterial genomes is important for virulence assessment, evolutionary analysis, and phage application development. The objective this study was to identify complete, inducible the cystic fibrosis (CF) clinical isolate Burkholderia cenocepacia H111. Using prophage-finding program PHAge Search Tool (PHAST), we identified three putative intact H111 sequence. Virions were readily isolated from culture supernatants following extended incubation. shotgun...
Carnobacterium divergens is frequently isolated from natural environments and a predominant species found in refrigerated foods, particularly meat, seafood, dairy. While there substantial interest using