A5044319486- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Phosphodiesterase function and regulation
- Calcium signaling and nucleotide metabolism
- Adipose Tissue and Metabolism
- Neurobiology and Insect Physiology Research
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Human Health and Disease
- Mesenchymal stem cell research
- Diet, Metabolism, and Disease
- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
- Electromagnetic Fields and Biological Effects
- Neuroscience of respiration and sleep
- Cholesterol and Lipid Metabolism
- Pain Mechanisms and Treatments
- Regulation of Appetite and Obesity
- Neurotransmitter Receptor Influence on Behavior
- Chemical synthesis and alkaloids
- Olfactory and Sensory Function Studies
- Planarian Biology and Electrostimulation
Hebrew University of Jerusalem
2025
Institute of Cell Biophysics
2014-2024
Pushchino Scientific Center for Biological Research
2024
Russian Academy of Sciences
2014
Transient receptor potential vanilloid 1 receptors (TRPV1) play a significant physiological role. The study of novel TRPV1 agonists and antagonists is essential. Here, we report on the characterization polypeptide based in vitro vivo experiments. We evaluated ability APHC1 APHC3 to inhibit using whole-cell patch clamp approach single cell Ca2+ imaging. In tests were performed assess biological effects temperature sensation, inflammation core body temperature. electrophysiological study, both...
In mammals, three genes encode IP3 receptors (IP3Rs), which are involved in agonist-induced Ca2+ signaling cells of apparently all types. Using the CRISPR/Cas9 approach for disruption two out IP3R HEK-293 cells, we generated monoclonal cell lines, IP3R1-HEK, IP3R2-HEK, and IP3R3-HEK, with single functional isoform, IP3R1, IP3R2, IP3R3, respectively. All engineered responded to ACh transients an “all-or-nothing” manner, suggesting that each isotype was capable mediating CICR. The sensitivity...
Type III taste cells are the only bud which express voltage-gated (VG) Ca2+ channels and employ Ca2+-dependent exocytosis to release neurotransmitters, particularly serotonin. The is a tightly packed cell population, wherein extracellular expected fluctuate markedly due electrical activity of cells. It currently unclear whether entry-driven synapse in type could be reliable enough at unsteady Ca2. Here we assayed depolarization-induced signals associated serotonin isolated varied Ca2+....
The current knowledge of electrogenesis in mesenchymal stromal cells (MSCs) remains scarce. Earlier, we demonstrated that MSCs from the human adipose tissue, transduction certain agonists involved phosphoinositide cascade. Its pivotal effector PLC generates DAG can regulate ion channels directly or via its derivatives, including arachidonic acid (AA). Here showed AA strongly hyperpolarized by stimulating instantly activating, outwardly rectifying TEA-insensitive K+ channels. Among...
The integrative study that included experimentation and mathematical modeling was carried out to analyze dynamic aspects of transient Ca2+ signaling induced by brief pulses GPCR agonists in mesenchymal stromal cells from the human adipose tissue (AD-MSCs). experimental findings argued for IP3/Ca2+-regulated release via IP3 receptors (IP3Rs) as a key mechanism mediating agonist-dependent transients. consistent circuit proposed formalize coupling agonist binding mobilization modeling. model...
The phosphoinositide 3-kinase (PI3K) is involved in regulation of multiple intracellular processes. Although the inhibitory analysis generally employed for validating a physiological role PI3K, increasing body evidence suggests that PI3K inhibitors can exhibit PI3K-unrelated activity as well. Here we studied Ca2+ signaling initiated by aminergic agonists variety different cells and analyzed effects inhibitor PI828 on cell responsiveness. It turned out inhibited transients elicited...
ABSTRACT The phosphoinositide 3-kinase (PI3K) is involved in regulation of multiple intracellular processes. Although the inhibitory analysis generally employed for validating a physiological role PI3K, increasing body evidence suggests that PI3K inhibitors can exhibit PI3K-unrelated activity as well. Here we studied effects inhibitor LY294002 and its inactive analogue LY303511 on Ca 2+ cAMP signals initiated by serotonin. In present study several monoclonal HEK293 cell lines were used,...