A5031534154- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Neuroscience and Neuropharmacology Research
- Redox biology and oxidative stress
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Ion Channels and Receptors
- Biochemical effects in animals
- Connexins and lens biology
- Adipose Tissue and Metabolism
- Heat shock proteins research
- Sulfur Compounds in Biology
- Calcium signaling and nucleotide metabolism
- bioluminescence and chemiluminescence research
- Glutathione Transferases and Polymorphisms
- Neuroscience of respiration and sleep
- Regulation of Appetite and Obesity
- Photoreceptor and optogenetics research
- Marine Biology and Environmental Chemistry
- RNA and protein synthesis mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Nicotinic Acetylcholine Receptors Study
Institute of Cell Biophysics
2014-2024
Institute of Biophysics
2017
Russian Academy of Sciences
1999-2015
Afferent output in type II taste cells is mediated by ATP liberated through ion channels. It widely accepted that pannexin 1 (Panx1) channels are responsible for release diverse cell types, including cells. While biophysical evidence implicates slow deactivation of following cells, recombinant Panx1 activates and deactivates rapidly. This inconsistency could indicate the cellular context specifies functioning. We cloned from murine tissue, heterologously expressed it three different lines:...
Conventional chemical synapses in the nervous system involve a presynaptic accumulation of neurotransmitter-containing vesicles, which fuse with plasma membrane to release neurotransmitters that activate postsynaptic receptors. In taste buds, type II receptor cells do not have conventional synaptic features but nonetheless show regulated their afferent neurotransmitter, ATP, through large-pore, voltage-gated channel, CALHM1. Immunohistochemistry revealed CALHM1 was localized points contact...
Three types of morphologically and functionally distinct taste cells operate in the mammalian bud. We demonstrate here expression two G-protein-coupled receptors from family C, CASR GPRC6A, tissue identify transcripts for both type I cells, no II only III by using SMART-PCR RNA amplification method at level individual cells. Type responded to calcimimetic NPS R-568, a stereoselective probe, with Ca2+ transients, whereas were not specifically responsive. Consistent these findings, certain...
cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium library and their inserts sequenced. The reconstructed sequence comprises 400 amino acids with calculated molecular mass of 46 026 Da. A homology was revealed between acid proteins involved in transfer hydrophobic ligands. Using situ hybridisation, mRNA expression detected layer supportive cells epithelium, apical region trachea, surface ciliated bronchial lung skin epidermis.
In mammals, three genes encode IP3 receptors (IP3Rs), which are involved in agonist-induced Ca2+ signaling cells of apparently all types. Using the CRISPR/Cas9 approach for disruption two out IP3R HEK-293 cells, we generated monoclonal cell lines, IP3R1-HEK, IP3R2-HEK, and IP3R3-HEK, with single functional isoform, IP3R1, IP3R2, IP3R3, respectively. All engineered responded to ACh transients an “all-or-nothing” manner, suggesting that each isotype was capable mediating CICR. The sensitivity...
Type III taste cells are the only bud which express voltage-gated (VG) Ca2+ channels and employ Ca2+-dependent exocytosis to release neurotransmitters, particularly serotonin. The is a tightly packed cell population, wherein extracellular expected fluctuate markedly due electrical activity of cells. It currently unclear whether entry-driven synapse in type could be reliable enough at unsteady Ca2. Here we assayed depolarization-induced signals associated serotonin isolated varied Ca2+....
The current knowledge of electrogenesis in mesenchymal stromal cells (MSCs) remains scarce. Earlier, we demonstrated that MSCs from the human adipose tissue, transduction certain agonists involved phosphoinositide cascade. Its pivotal effector PLC generates DAG can regulate ion channels directly or via its derivatives, including arachidonic acid (AA). Here showed AA strongly hyperpolarized by stimulating instantly activating, outwardly rectifying TEA-insensitive K+ channels. Among...
The specific odor-binding glycoproteins have been isolated from rat olfactory epithelium. They consist of two subunits, gp88 and gp55. Subunit is capable odorant binding.
The integrative study that included experimentation and mathematical modeling was carried out to analyze dynamic aspects of transient Ca2+ signaling induced by brief pulses GPCR agonists in mesenchymal stromal cells from the human adipose tissue (AD-MSCs). experimental findings argued for IP3/Ca2+-regulated release via IP3 receptors (IP3Rs) as a key mechanism mediating agonist-dependent transients. consistent circuit proposed formalize coupling agonist binding mobilization modeling. model...