A5101490456- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- High Altitude and Hypoxia
- Plant Virus Research Studies
- Genetic and phenotypic traits in livestock
Ministry of Agriculture and Rural Affairs
2023-2024
Northwest A&F University
2018-2024
Sheep were domesticated in the Fertile Crescent and then spread globally, where they have been encountering various environmental conditions. The Tibetan sheep has adapted to high altitudes on Qinghai-Tibet Plateau over past 3000 years. To explore genomic variants associated with high-altitude adaptation sheep, we analyzed Illumina short-reads of 994 whole genomes representing ∼ 60 breeds/populations at varied altitudes, PacBio High fidelity (HiFi) reads 13 breeds, 96 transcriptomes from 12...
Graphical Abstract Mouse modeling could offer a powerful in vivo investigation tool for validating the functional role of candidate genes and genomic variants detected animal livestock species via multi-omic analyses. In this Commentary, authors discuss potential transgenic genome-edited mice as significant models outcomes
Abstract The ability to alter single bases without DNA double strand breaks provides a potential solution for multiplex editing of livestock genomes quantitative traits. Here, we report using base system, Base Editor 3 (BE3), induce nonsense codons (C-to-T transitions) at four target sites in caprine FGF5. All five progenies produced from microinjected single-cell embryos had alleles with targeted mutation and yielded expected phenotypes. effectiveness BE3 make changes varied considerably...