A5055773703- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Algal biology and biofuel production
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
Michigan State University
2012-2025
Following N deprivation, microalgae accumulate triacylglycerols (TAGs). To gain mechanistic insights into this phenomenon, we identified mutants with reduced TAG content following deprivation in the model alga Chlamydomonas reinhardtii. In one of mutants, disruption a galactoglycerolipid lipase-encoding gene, designated PLASTID GALACTOGLYCEROLIPID DEGRADATION1 (PGD1), was responsible for primary phenotype: content, altered composition, and turnover. The recombinant PGD1 protein, which...
Tocopherol synthesis requires phytyl diphosphate derived from phytol esterified to chlorophyll metabolites. The >600-member Arabidopsis thaliana α/β hydrolase (ABH) gene family contains 4 members that can release metabolites in vitro; however, only pheophytinase (PPH) affects tocopherol when mutated, reducing seed tocopherols by 5%. We report the biochemical analysis of 2 previously uncharacterized ABHs, dephytylase (CLD2) and CLD3, their respective mutants singly combinations with pph cld1...