A5100337384- Plant Reproductive Biology
- Plant Molecular Biology Research
- Biochemical and biochemical processes
- Enzyme-mediated dye degradation
- Lipid metabolism and biosynthesis
- Chemical Synthesis and Analysis
- Photosynthetic Processes and Mechanisms
- Advanced Breast Cancer Therapies
- Medicinal Plants and Neuroprotection
- Plant pathogens and resistance mechanisms
- Ecology and Conservation Studies
- Plant Physiology and Cultivation Studies
- Pluripotent Stem Cells Research
- Natural product bioactivities and synthesis
- Advanced biosensing and bioanalysis techniques
- Infection Control and Ventilation
- Cephalopods and Marine Biology
- Drug Transport and Resistance Mechanisms
- COVID-19 epidemiological studies
- Chemical Synthesis and Reactions
- Environmental Chemistry and Analysis
- Lepidoptera: Biology and Taxonomy
- CRISPR and Genetic Engineering
- Antioxidant Activity and Oxidative Stress
- Advanced Synthetic Organic Chemistry
Michigan State University
2022-2025
Columbia University Irving Medical Center
2023-2024
Columbia University
2023
Samsung (South Korea)
2013-2015
University of British Columbia
2009-2013
Inha University
2004
Acyl-CoA Synthetase (ACOS) genes are related to 4-coumarate:CoA ligase (4CL) but have distinct functions. The Arabidopsis thaliana ACOS5 protein is in clade A of ACOS proteins, the most closely 4CL proteins. This contains putative nonperoxisomal enzymes conserved several angiosperm lineages and moss Physcomitrella patens. Although its function unknown, preferentially expressed flowers all angiosperms examined. Here, we show that an acos5 mutant produced no pollen mature anthers seeds by...
Abstract The precise structure of the sporopollenin polymer that is major constituent exine, outer pollen wall, remains poorly understood. Recently, characterization Arabidopsis thaliana genes and corresponding enzymes involved in exine formation has demonstrated role fatty acid derivatives as precursors building units. Fatty acyl-CoA esters synthesized by ACYL-COA SYNTHETASE5 (ACOS5) are condensed with malonyl-CoA POLYKETIDE SYNTHASE A (PKSA) PKSB to yield α-pyrone polyketides required for...
Abstract Plant type III polyketide synthases (PKSs) catalyze the condensation of malonyl-CoA units with various CoA ester starter molecules to generate a diverse array natural products. The fatty acyl-CoA esters synthesized by Arabidopsis thaliana ACYL-COA SYNTHETASE5 (ACOS5) are key intermediates in biosynthesis sporopollenin, major constituent exine outer pollen wall. By coexpression analysis, we identified two PKS genes, POLYKETIDE SYNTHASE A (PKSA) and PKSB (also known as LAP6 LAP5,...
Cyclin-dependent kinase 4 and 6 inhibitors (CDK4/6i) are key therapeutic agents in the management of metastatic hormone-receptor-positive breast cancer. However, emergence drug resistance limits their long-term efficacy. Here, we show that cancer cells develop CDK4/6i via a sequential two-step process E2F activation. This entails retinoblastoma (Rb)-protein degradation, followed by c-Myc-mediated amplification transcriptional activity. treatment halts cell proliferation an Rb-dependent...
Tocopherol synthesis requires phytyl diphosphate derived from phytol esterified to chlorophyll metabolites. The >600-member Arabidopsis thaliana α/β hydrolase (ABH) gene family contains 4 members that can release metabolites in vitro; however, only pheophytinase (PPH) affects tocopherol when mutated, reducing seed tocopherols by 5%. We report the biochemical analysis of 2 previously uncharacterized ABHs, dephytylase (CLD2) and CLD3, their respective mutants singly combinations with pph cld1...
• Sporopollenin is the main constituent of exine layer spore and pollen walls. Recently, several Arabidopsis genes, including polyketide synthase A (PKSA), which encodes an anther-specific chalcone synthase-like enzyme (ASCL), have been shown to be involved in sporopollenin biosynthesis. The genome moss Physcomitrella patens contains putative orthologs biosynthesis genes. We analyzed available P. expressed sequence tag (EST) data for genes studied enzymatic properties reaction mechanism...
Significance Tocopherols (vitamin E) are plant-synthesized, lipid-soluble antioxidants whose dietary intake, primarily from seed oils, is essential for human health. contain a phytol-derived hydrophobic tail in vivo source has been elusive. The most significant genome-wide association signal Arabidopsis tocopherols identified an uncharacterized, seed-specific esterase (VTE7) localized to the chloroplast envelope, where tocopherol synthesis occurs. VTE7 disruption and overexpression had large...
The product yield and titers of biological processes involving the conversion biomass to desirable chemicals can be limited by environmental stresses encountered microbial hosts used for bioconversion. One these main is growth inhibition due exposure low pH conditions. In order circumvent this problem, understanding mechanisms involved in acid stress response tolerance essential. Characterisation wild yeasts that have a natural ability resist such harsh conditions will pave way understand...
The SARS-CoV-2 pandemic has presented new challenges to food manufacturers. During the early phase of pandemic, several large outbreaks coronavirus disease 2019 (COVID-19) occurred in manufacturing plants resulting deaths and economic loss, with approximately 15% personnel diagnosed as asymptomatic for COVID-19. Spread by presymptomatic individuals been implicated In March 2020, we assisted implementation environmental monitoring programs zones 3 4 116 production facilities. All...
S. Kim and Kim, J. Chem. Soc., Commun., 1986, 719 DOI: 10.1039/C39860000719
Ochnaflavone, a naturally occurring biflavonoid composed of two units apigenin (5,7,4'-trihydroxyflavone) joined via C-O-C linkage, was first synthesized and evaluated its inhibitory activity on <TEX>$PGE_2$</TEX> production. Total synthesis accomplished through modified Ullmann diaryl ether formation as key step. Coupling reactions 4'-halogenoflavones 3'-hydroxy-5,7,4'-trimethoxyflavone were explored in diverse reaction conditions. The 4'-fluoro-5,7-dimethoxyflavone (2c) (2d)...
Abstract CDK7 plays a pivotal role in cell proliferation, serving as both cell-cycle CDK regulator and transcription machinery component. Clinical trials are currently exploring inhibitors (CDK7i) second-line treatment for hormone receptor-positive (HR+) breast cancer post-CDK4/6 inhibitor (CDK4/6i) therapy progression first-line triple-negative (TNBC). This study evaluates the impact of CDK7i on CDKs activities synergistic potential combination with CDK4/6i. Kinetic analyses indicate that...
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