Abstract Sustainable production of oleochemicals requires establishment cell factory platform strains. The yeast Saccharomyces cerevisiae is an attractive as new strains can be rapidly implemented into existing infrastructures such bioethanol plants. Here we show high-level free fatty acids (FFAs) in a factory, and the alkanes alcohols from its descendants. engineered strain produces up to 10.4 g l −1 FFAs, which highest reported titre date. Furthermore, through screening specific pathway...

The widely used pESC vector series (Stratagene, La Jolla, CA, USA) with the bidirectional GAL1/GAL10 promoter provides possibility of simultaneously expressing two different genes from a single in Saccharomyces cerevisiae. This system can be induced by galactose and is repressed glucose. Since S. cerevisiae prefers glucose as carbon source, since its growth rate higher than galactose-containing media, we compared evaluated seven promoters expressed during on (pTEF1, pADH1, pTPI1, pHXT7,...

ABSTRACT Acetyl coenzyme A (acetyl-CoA) carboxylase (ACCase) plays a central role in carbon metabolism and has been the site of action for development therapeutics or herbicides, as its product, malonyl-CoA, is precursor production fatty acids other compounds. Control Acc1 activity yeast Saccharomyces cerevisiae occurs mainly at two levels, i.e., regulation transcription repression by Snf1 protein kinase level. Here, we demonstrate strategy improving ACCase S. abolishing posttranslational...

Establishing efficient synthetic pathways for microbial production of biochemicals is often hampered by competing and/or insufficient precursor supply. Compartmentalization in cellular organelles can isolate from pathways, and provide a compact suitable environment biosynthesis. Peroxisomes are where fatty acids degraded, process that inhibited under typical fermentation conditions making them an interesting workhouse fatty-acid-derived molecules. Here, we show targeting to peroxisomes...

Abstract Genome-scale metabolic models (GEMs) have been widely used for quantitative exploration of the relation between genotype and phenotype. Streamlined integration enzyme constraints proteomics data into such was first enabled by GECKO toolbox, allowing study phenotypes constrained protein limitations. Here, we upgrade toolbox in order to enhance with any organism a compatible GEM reconstruction. With this, enzyme-constrained budding yeasts Saccharomyces cerevisiae , Yarrowia lipolytica...

The construction of mitotically stable yeast strains for heterologous gene or pathway expression often requires chromosomal integration. However, transcription levels vary between different chromosome regions. We therefore characterized 20 integration sites the Sacchromyces cerevisiae genome by inserting lacZ as a reporter under control two promoters and determining through enzyme activity measurement. An up to 8.7-fold difference was detected conferring lowest highest expression,...

SUMMARY The oxidative burst, a transient and rapid accumulation of reactive oxygen species (ROS), is widespread defence mechanism higher plants against pathogen attack. There increasing evidence that the necrotrophic fungal Botrytis cinerea itself generates ROS, this capability could contribute to virulence fungus. Two potential H 2 O ‐generating systems were studied with respect their impact on interaction B. its host plant Phaseolus vulgaris . A Cu‐Zn‐superoxide dismutase gene ( bcsod1 )...

The micrographic phytopathogen Botrytis cinerea causes gray mold diseases in a large number of dicotyledonous crop plants and ornamentals. Colonization host tissue is accompanied by rapid killing plant cells ahead the growing hyphen, probably caused secretion nonspecific phytotoxins, e.g., sesquiterpene botrydial. Although all pathogenic strains tested so far had been shown to secrete botrydial although toxin comparable necrotic lesions as infection fungus, role process has not elucidated...

Abstract Background Sesquiterpenes are a class of natural products with diverse range attractive industrial proprieties. Due to economic difficulties sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-santalene precursor sesquiterpenes relevant commercial applications. Here, we construct an Saccharomyces cerevisiae cell factory for production. Results A multistep metabolic...

Due to an increasing demand of transportation fuels, a lower availability cheap crude oil and lack sustainability fossil gradual shift from petroleum based fuels towards alternative renewable fuel resources will be required in the near future. Fatty acid ethyl esters (FAEEs) have properties similar current diesel could therefore form important contribution development sustainable It is develop novel cell factories for efficient production FAEEs their precursors. Here, Saccharomyces...

The establishment of a heterologous pathway in microbial host for the production industrially relevant chemicals at high titers and yields requires efficient adjustment central carbon metabolism to ensure that flux is directed toward product interest. This can be achieved through regulation key branch points metabolic networks, here we present novel approach dynamic modulation enzyme expression levels. based on hierarchical control system around intermediate malonyl-CoA. upper level ensures...

As a key intracellular metabolite, acetyl-coenzyme A (acetyl-CoA) plays major role in various metabolic pathways that link anabolism and catabolism. In the yeast Saccharomyces cerevisiae, acetyl-CoA involving metabolism is compartmentalized, may vary with nutrient supply of cell. Membranes separating compartments are impermeable to no direct transport between occurs. Thus, without carnitine glyoxylate shunt sole possible route for transferring from cytosol or peroxisomes into mitochondria....

Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows use yeast cells production that are high-value materials be used in a variety industrial applications. products WSs include acid ethyl esters, which directly as biodiesel.Here, heterologous derived five different organisms were successfully expressed evaluated their substrate preference Saccharomyces cerevisiae. We investigated...

Abstract Understanding the genetic regulatory code governing gene expression is an important challenge in molecular biology. However, how individual coding and non-coding regions of structure interact contribute to mRNA levels remains unclear. Here we apply deep learning on over 20,000 datasets examine controlling abundance 7 model organisms ranging from bacteria Human. In all organisms, can predict directly DNA sequence, with up 82% variation transcript encoded structure. By searching for...

Terpenes have various applications as fragrances, cosmetics and fuels. One of the most prominent examples is sesquiterpene farnesene, which can be used diesel substitute in its hydrogenated form farnesane. Recent metabolic engineering efforts enabled efficient production several terpenes Saccharomyces cerevisiae Escherichia coli. Plant terpene synthases take on an essential function for they catalyze specific conversion universal precursor farnesyl diphosphate (FPP) to interest thereby...

Abstract Baker’s yeast Saccharomyces cerevisiae is an attractive cell factory for production of chemicals and biofuels. Many different products have been produced in this by reconstruction heterologous biosynthetic pathways; however, endogenous metabolism itself involves many metabolites industrial interest, de-regulation pathways to ensure efficient carbon channelling such therefore high interest. Furthermore, these may serve as precursors the biosynthesis complex natural products, hence...