A5045637694- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Genomics, phytochemicals, and oxidative stress
- Plant Molecular Biology Research
- Microbial Natural Products and Biosynthesis
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Plant Surface Properties and Treatments
- Sirtuins and Resveratrol in Medicine
- Marine and coastal plant biology
- Ziziphus Jujuba Studies and Applications
- Biological Activity of Diterpenoids and Biflavonoids
- Traditional and Medicinal Uses of Annonaceae
- Chemical Synthesis and Analysis
- Fern and Epiphyte Biology
- Phytochemistry and Biological Activities
- Wound Healing and Treatments
- Biochemical Analysis and Sensing Techniques
- Environmental Toxicology and Ecotoxicology
- Microbial Metabolic Engineering and Bioproduction
- Trypanosoma species research and implications
- Lichen and fungal ecology
- Hemophilia Treatment and Research
- Analytical Methods in Pharmaceuticals
University of Regina
2010-2024
Tohoku Medical and Pharmaceutical University
1999-2007
Utah State University
2004
University of Utah
2003-2004
Rice University
2003
University of Washington
2003
Yale University
2003
The University of Tokyo
1999
Children's Medical Center
1999
Seoul National University
1994-1998
Abstract Plant type III polyketide synthases (PKSs) catalyze the condensation of malonyl-CoA units with various CoA ester starter molecules to generate a diverse array natural products. The fatty acyl-CoA esters synthesized by Arabidopsis thaliana ACYL-COA SYNTHETASE5 (ACOS5) are key intermediates in biosynthesis sporopollenin, major constituent exine outer pollen wall. By coexpression analysis, we identified two PKS genes, POLYKETIDE SYNTHASE A (PKSA) and PKSB (also known as LAP6 LAP5,...
Corticosteroids are widely used therapeutic agents that have as a major side-effect the impairment of wound healing. Two hypotheses were tested: 1) antiinflammatory corticosteroids decrease local insulin-like growth factor-I (IGF-I) response to injury; and 2) locally administered IGF-I would overcome methylprednisolone-mediated suppression The concentration was measured in fluid from wire mesh cylinder wounds rats given saline or methylprednisolone im. Rats receiving 8 16 mg had decreased...
Chalcone synthase (CHS) and stilbene (STS) catalyse condensation reactions of p-coumaroyl-CoA three C(2) units from malonyl-CoA up to a common tetraketide intermediate but then different cyclization produce naringenin chalcone resveratrol respectively. On the basis sequence alignment with other condensing enzymes including 3-ketoacyl-(acyl carrier protein) synthases polyketide fatty-acid synthases, site-directed mutagenesis was performed on active-site G(372)FGPG loops in CHS STS. The...
• Sporopollenin is the main constituent of exine layer spore and pollen walls. Recently, several Arabidopsis genes, including polyketide synthase A (PKSA), which encodes an anther-specific chalcone synthase-like enzyme (ASCL), have been shown to be involved in sporopollenin biosynthesis. The genome moss Physcomitrella patens contains putative orthologs biosynthesis genes. We analyzed available P. expressed sequence tag (EST) data for genes studied enzymatic properties reaction mechanism...
Chalcone synthase (CHS) and stilbene (STS) are related plant polyketide synthases belonging to the CHS superfamily. STS catalyze common condensation reactions of p ‐coumaroyl‐CoA three C 2 ‐units from malonyl‐CoA but different cyclization produce naringenin chalcone resveratrol, respectively. Using purified Pueraria lobata Arachis hypogaea overexpressed in Escherichia coli , bisnoryangonin (BNY, derailed lactone after two condensations) ‐coumaroyltriacetic acid (the were detected reaction...
The leaf extract of Biota orientalis showed potent PAF receptor binding antagonistic activity in our previous screening studies on 234 Korean medicinal plants using rabbit platelet tests. activity-guided purification the plant resulted isolation six compounds, including two active substances. chemical structures compounds isolated were established by and spectrometric analyses as dotriacontane, totarol, 8β-hydroxy-3-oxopimar-15-ene, cedrol (IC50 = 1.3 × 10-5M), pinusolide 2.52 10-7M),...
Stilbene synthase [STS; Enzyme Commis sion (EC) 2.3.1.95] and chalcone (CHS; EC 2.3.1.74) are members of the type III polyketide synthases (PKSs) plant-specific enzymes.1 CHS is widely found in higher plants plays a key role flavonoid biosynthesis by supplying to downstream enzymes. In contrast, limited number have STS essential for synthesis resveratrol utilized stilbenoid biosynthesis.2 The superfamily, including STS, produce linear intermediates common catalytic mechanism where coenzyme A...
Sporopollenin is the main constituent of exine layer spore and pollen walls. The anther-specific chalcone synthase-like (ASCL) enzyme Physcomitrella patens, PpASCL, has previously been implicated in biosynthesis sporopollenin, perine, two outermost layers moss cell wall. We made targeted knockouts corresponding gene, phenotypically characterized ascl sporophytes spores at different developmental stages. Ascl plants developed normally until late sporophytic development, when produced were...
Corticosteroids are widely used therapeutic agents that have as a major side-effect the impairment of wound healing. Two hypotheses were tested: 1) antiinflammatory corticosteroids decrease local insulin-like growth factor-I (IGF-I) response to injury; and 2) locally administered IGF-I would overcome methylprednisolone-mediated suppression The concentration was measured in fluid from wire mesh cylinder wounds rats given saline or methylprednisolone im. Rats receiving 8 16 mg had decreased...
cDNAs encoding geranylgeranyl diphosphate synthase (GGPPS) of two diterpene-producing plants, Scoparia dulcis and Croton sublyratus, have been isolated using the homology-based polymerase chain reaction (PCR) method. Both clones contained highly conserved aspartate-rich motifs (DDXX(XX)D) their N-terminal residues exhibited characteristics chloroplast targeting sequence. When expressed in Escherichia coli, both full-length truncated proteins which putative sequence was deleted catalyzed...
The plant type III polyketide synthases (PKSs), which produce diverse secondary metabolites with different biological activities, have successfully co-evolved land plants. To gain insight into the roles that ancestral PKSs played during early evolution of plants, we cloned and characterized PpORS from moss Physcomitrella. has been proposed to closely resemble most recent common ancestor PKSs. condenses a very long chain fatty acyl-CoA four molecules malonyl-CoA catalyzes decarboxylative...
Anther-specific chalcone synthase-like enzyme (ASCL), an ancient plant type III polyketide synthase, is involved in the biosynthesis of sporopollenin, stable biopolymer found exine layer wall a spore or pollen grain. The gene encoding synthase 1 from Hypericum perforatum (HpPKS1) was previously shown to be expressed mainly young flower buds, but also leaves and other tissues at lower levels. Angiosperm ASCLs, identified by sequence phylogenetic analyses, are divided into two sister clades,...
Small-molecule oxidosqualene cyclase (OSC) inhibitors were found to be effective in assays against cloned OSC-like enzymes from human pathogens. A combinatorial library was prepared and used identify lead compounds that inhibit the growth of Trypanosoma cruzi, Leishmania mexicana amazonensis, Pneumocystis carinii culture. Selectivity for microorganisms preference mammalian cells observed.
Abstract The enigmatic moss, Takakia lepidozioides , possesses a particular type III polyketide synthase, ASCL (Anther-Specific Chalcone synthase-Like), that is an identifying marker for genuine sporopollenin in the walls of embryophyte spores and pollen grains. By contrast, survey all algae with sequenced genomes confirms they do not possess and, therefore, their spore are composed sporopollenin.
Chalcone synthase (CHS) and stilbene (STS) catalyse condensation reactions of p-coumaroyl-CoA three C2 units from malonyl-CoA up to a common tetraketide intermediate but then different cyclization produce naringenin chalcone resveratrol respectively. On the basis sequence alignment with other condensing enzymes including 3-ketoacyl-(acyl carrier protein) synthases polyketide fatty-acid synthases, site-directed mutagenesis was performed on active-site G372FGPG loops in CHS STS. The CHS-P375G...