A5012890852- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Electrowetting and Microfluidic Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- VLSI and Analog Circuit Testing
- Visual perception and processing mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- VLSI and FPGA Design Techniques
- COVID-19 Clinical Research Studies
- Interconnection Networks and Systems
- SARS-CoV-2 and COVID-19 Research
- 3D IC and TSV technologies
- vaccines and immunoinformatics approaches
- Modular Robots and Swarm Intelligence
- Receptor Mechanisms and Signaling
- Olfactory and Sensory Function Studies
- Quantum-Dot Cellular Automata
- 3D Printing in Biomedical Research
- Gaze Tracking and Assistive Technology
- Urticaria and Related Conditions
- Gout, Hyperuricemia, Uric Acid
University of Science and Technology Beijing
2024
Washington University in St. Louis
2007-2023
Tsinghua University
2018-2023
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine
2019-2022
Shanghai University of Traditional Chinese Medicine
2021-2022
Army Medical University
1995
Little is known regarding differences in the gut microbiomes of rheumatoid arthritis (RA) patients and healthy cohorts China. This study aimed to identify fecal 66 Chinese with RA 60 controls. The V3-V4 variable regions bacterial 16S rRNA genes were sequenced Illumina system define composition. alpha-diversity index microbiome was significantly lower than that control group. genera Bacteroides (p=0.02202) Escherichia-Shigella (p=0.03137) more abundant patients. In contrast, Lactobacillus...
Abstract Drug screening based on in-vitro primary tumor cell culture has demonstrated potential in personalized cancer diagnosis. However, the limited number of cells, especially from patients with early stage cancer, hindered widespread application this technique. Hence, we developed a digital microfluidic system for drug using cells and established working protocol precision medicine. Smart control logic was to increase throughput decrease its footprint parallelly screen three drugs 4 × cm...
Abstract Neocortical computations underlying vision are performed by a distributed network of functionally specialized areas. Mouse visual cortex, dense interareal that exhibits hierarchical properties, comprises subnetworks interconnecting distinct processing streams. To determine the layout mouse hierarchy, we have evaluated laminar patterns formed axonal projections originating in each ten Reciprocally connected pairs areas exhibit feedforward/feedback relationships consistent with...
In the big brown bat ( Eptesicus fuscus), conditioning with acoustic stimuli followed by electric leg-stimulation causes shifts in frequency-tuning curves and best frequencies (hereafter BF shifts) of collicular cortical neurons, i.e., reorganization cochleotopic (frequency) maps inferior colliculus (IC) auditory cortex (AC). The shift recovers 180 min after conditioning, but lasts longer than 26 h. is not caused as AC inactivated during conditioning. Therefore it has been concluded that...
In the awake big brown bat, 30 min auditory fear conditioning elicits conditioned heart rate decrease and long-term best frequency (BF) shifts of cortical neurons toward tone; 15 subthreshold BF that can be augmented by acetylcholine. The causes stress an increase in serotonin (5-HT) level. Serotonergic raphe nuclei associated with project to cerebral cortex cholinergic basal forebrain. Recently, it has been shown 5-HT(2A) receptors are mostly expressed on pyramidal their activation improves...
Here, we evaluated the immune properties of HLA-A2 restricted CD8+ T cell epitopes containing mutations from B.1.1.7, and furthermore performed a comprehensive analysis SARS-CoV-2 specific responses COVID-19 convalescent patients vaccinees recognizing ancestral Wuhan strain compared to B.1.1.7. First, most predicted showed proper binding with HLA-A2, whereas B.1.1.7 had lower capability than those strain. In addition, these peptides could effectively induce activation cytotoxicity cells. Our...
Layer 1 of V1 has been shown to receive locomotion-related signals from the dorsal lateral geniculate (dLGN) and posterior (LP) thalamic nuclei ( Roth et al., 2016 ). Inputs dLGN terminate in M2+ patches while inputs LP target M2- interpatches D'Souza 2019 ) suggesting that motion related are processed distinct networks. Here, we investigated by calcium imaging head-fixed awake mice whether L2/3 neurons underneath L1 modules differentially activated locomotion, networks feedback connections...
Layer 1 of V1 has been shown to receive locomotion-related signals from the dorsal lateral geniculate (dLGN) and posterior (LP) thalamic nuclei (Roth et al., 2016). Inputs dLGN terminate in M2+ patches while inputs LP target M2− interpatches (D’Souza 2019) suggesting that motion related are processed distinct networks. Here, we investigated by calcium imaging head-fixed awake mice whether L2/3 neurons underneath L1 modules differentially activated locomotion, networks feedback connections...
Layer 1 of V1 has been shown to receive locomotion-related signals from the dorsal lateral geniculate (dLGN) and posterior (LP) thalamic nuclei (Roth et al., 2016). Inputs dLGN terminate in M2+ patches while inputs LP target M2− interpatches (D’Souza 2019) suggesting that motion related are processed distinct networks. Here, we investigated by calcium imaging head-fixed awake mice whether L2/3 neurons underneath L1 modules differentially activated locomotion, networks feedback connections...
Reorganization of the frequency map in central auditory system is based on shifts best frequencies (BFs; hereafter, BF shifts), together with frequency-response curves, neurons. In big brown bat, conditioning acoustic stimulation followed by electric leg-stimulation causes collicular and cortical The shift develops quickly short term, whereas slowly long term. acetycholine level cortex must be high during to develop these shifts. We studied effect atropine (an antagonist muscarinic...
The postrhinal area (POR) is a known center for integrating spatial with nonspatial visual information and possible hub influencing landmark navigation by affective input from the amygdala. This may involve specific circuits within muscarinic acetylcholine receptor 2 (M2)-positive (M2 + ) or M2 – modules of POR that associate inputs thalamus, cortex, amygdala, send outputs to entorhinal cortex. Using anterograde retrograde labeling conventional viral tracers in male female mice, we found all...
In big brown bats, tone-specific plastic changes [best frequency (BF) shifts] of cortical and collicular neurons can be evoked by auditory fear conditioning, repetitive acoustic stimuli or electric stimulation. It has been shown that acetylcholine (ACh) plays an important role in evoking large long-term BF shifts. However, the N-methyl-d-aspartate (NMDA) receptors shifts not yet studied. We found 1) NMDA applied to cortex (AC) inferior colliculus (IC) augmented responses, as ACh did, whereas...
Experience-dependent plasticity in the central sensory systems depends on activation of both and neuromodulatory systems. Sensitization or nonspecific augmentation auditory neurons elicited by pseudo-conditioning with unpaired conditioning tonal (CS) unconditioned electric leg (US) stimuli is quite different from tone-specific plasticity, called best frequency (BF) shifts, fear paired CS US. Therefore neural circuits eliciting must be that BF shifts. We first examined plastic changes...
According to the complexity of human physiology and variability among individuals, e.g., genes, environment, lifestyle exposures, etc., personalized medicine aims synthesize specific efficacious drug for each individual patient. For synthesizing medicine, customized solutions with concentrations are required. Equipped advantages in saving costly reagents rare samples, microfluidic biochips promising generating different medicine. On one hand, digital require programming control driving...
Recent years have witnessed significant advances brought by microfluidic biochips in automating biochemical protocols. Accurate preparation of fluid samples is an essential component these protocols, where concentration prediction and generation are critical. Equipped with the advantages convenient fabrication control, mixers demonstrate huge potential sample preparation. Although finite element analysis (FEA) most commonly used simulation method for accurate a given mixer, it time-consuming...
Paper-based digital microfluidic biochips (P-DMFBs) have recently emerged as a promising low-cost and fast-responsive platform for biochemical assays. In P-DMFBs, electrodes control lines are printed on piece of photograph paper using an inkjet printer carbon nanotubes (CNTs) conductive ink. Compared with traditional (DMFBs), P-DMFBs enjoy significant advantages, such faster in-place fabrication ink, lower costs, better disposability. Since CNT the same side this paper, critical design...
ABSTRACT Neocortical circuit computations underlying active vision are performed by a distributed network of reciprocally connected, functionally specialized areas. Mouse visual cortex is dense, hierarchically organized network, comprising subnetworks that form preferentially interconnected processing streams. To determine the detailed layout mouse hierarchy, laminar patterns formed interareal axonal projections, originating in each ten areas were analyzed. Reciprocally connected pairs...
Experience-dependent plastic changes in the central sensory systems are due to activation of both and neuromodulatory systems. Nonspecific cortical auditory neurons elicited by pseudoconditioning quite different from tone-specific fear conditioning. Therefore neural circuit evoking nonspecific must also be that changes. We first examined response properties big brown bat with unpaired tonal (CS(u)) electric leg (US(u)) stimuli found it CS(u) (a heart-rate decrease, an increase, a broadening...
Escape routing, especially ordered escape is a critical design stage for both printed circuit boards (PCBs) and integrated fan-out (InFO) wafer-level chip-scale packages. Previous works formulate routing as boolean satisfiability (SAT) or integer linear programming (ILP) problems. Although optimal solutions can be obtained by above-mentioned approaches, the runtime unacceptable large-scale designs due to exponential time complexity of SAT ILP solvers. In this paper, we first attempt address...
Interactions between feedback connections from higher cortical areas and local horizontal within primary visual cortex (V1) were shown to play a role in contextual processing different behavioral states. Layer 1 (L1) is an important part of the underlying network. This cell-sparse layer target inputs, nexus for contacts onto apical dendrites projection neurons layers below. Importantly, L1 site coupling inputs outside world with internal information. To determine whether all these circuit...