A5019282205- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Microfluidic and Capillary Electrophoresis Applications
- Cell Image Analysis Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Genetics, Bioinformatics, and Biomedical Research
- Protein Structure and Dynamics
- Microfluidic and Bio-sensing Technologies
- vaccines and immunoinformatics approaches
- Innovative Microfluidic and Catalytic Techniques Innovation
- Silk-based biomaterials and applications
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- CAR-T cell therapy research
- Transgenic Plants and Applications
- Computational Drug Discovery Methods
- Cell Adhesion Molecules Research
- Polymer crystallization and properties
- Cellular Mechanics and Interactions
- Polymer Science and PVC
- Enzyme Structure and Function
- Advanced Polymer Synthesis and Characterization
- Genomics and Phylogenetic Studies
- Biosimilars and Bioanalytical Methods
Amgen (United States)
2019-2024
Scripps Research Institute
2008
California Institute of Technology
1999-2007
Pennsylvania State University
1999
Early identification of antibody candidates with drug-like properties is essential for simplifying the development safe and effective therapeutics. For subcutaneous administration, it important to identify low self-association enable their formulation at high concentration while maintaining viscosity, opalescence, aggregation. Here, we report an interpretable machine learning model predicting (IgG1) variants viscosity using only sequences variable (Fv) regions. Our was trained on data (>100...
We describe a novel, simple method for the photolithographic patterning of cell-adhesive proteins. Intrinsically photoreactive proteins are synthesized in Escherichia coli through incorporation non-canonical, photosensitive amino acid para-azidophenylalanine. Upon ultraviolet irradiation at 365 nm, form cross-linked films with elastic moduli that can be tuned by varying concentration expression medium. Films these directly patterned using standard techniques. demonstrate utility this protein...
Biologic drug discovery pipelines are designed to deliver protein therapeutics that have exquisite functional potency and selectivity while also manifesting biophysical characteristics suitable for manufacturing, storage, convenient administration patients. The ability use computational methods predict properties from sequence, potentially in combination with high throughput assays, could decrease timelines increase the success rates therapeutic developability engineering by eliminating...
Generative biology combines artificial intelligence (AI), advanced life sciences technologies, and automation to revolutionize the process of designing novel biomolecules with prescribed properties, giving drug discoverers ability escape limitations during design next-generation protein therapeutics. Significant hurdles remain, namely: (i) inherently complex nature discovery, (ii) bewildering number promising computational experimental techniques that have emerged in past several years,...
Abstract The unsaturated amino acid 2‐amino‐3‐methyl‐4‐pentenoic ( E ‐Ile) was prepared in the form of its (2 S , 3 ),(2 R ) and stereoisomeric pairs. translational activities SS ‐ ‐Ile SR were assessed an E. coli strain rendered auxotrophic for isoleucine. incorporated into test protein mouse dihydrofolate reductase (mDHFR) place isoleucine at a rate up to 72 %; yielded no conclusive evidence incorporation. ATP/PP i exchange assays indicated that activated by isoleucyl‐tRNA synthetase...
We introduce μVAST, a high-throughput acoustic microstreaming platform using second-order to induce fluid transport and measure the viscosity of 16 samples, automating process flows in drug development, materials manufacturing production.
We are entering an era in which therapeutic proteins assembled using building block-like strategies, with no standardized schema to discuss these formats. Existing nomenclatures, like AbML, sacrifice human readability for precision. Therefore, considering even a dozen such formats, combination hundreds of possible targets, can create confusion and increase the complexity drug discovery. To address this challenge, we introduce Verified Taxonomy Antibodies (VERITAS). This classification...
Multispecific antibodies, often composed of three to five polypeptide chains, have become increasingly relevant in the development biotherapeutics. These molecules mechanisms action that include redirecting T cells tumors and blocking multiple pathogenic mediators simultaneously. One major challenges for asymmetric multispecific antibodies is generating a high proportion correctly paired antibody during production. To understand causes effects chain mispairing impurities difficult express...
Abstract Oxidation reactions are one of the main reasons for failure polymeric materials. Antioxidants, compounds designed to protect against oxidation, must meet three requirements success: (1) an efficient antioxidative mechanism, (2) compatibility with oxidizing polymer, and (3) permanence Within polymer. Common antioxidants low molecular weight materials that can easily diffuse, leach, or evaporate from polymer they protect. An increase in antioxidant not only decreases diffusion...
In this investigation, we tested the hypothesis that a physiologically based pharmacokinetic (PBPK) model incorporating measured in vitro metrics of off-target binding can largely explain inter-antibody variability monoclonal antibody (mAb) pharmacokinetics (PK). A diverse panel 83 mAbs was evaluated for PK wild-type mice and subjected to 10 assays measure major physiochemical attributes. After excluding target-mediated elimination immunogenicity, 56 remaining with an eight-fold area under...