A5107459905- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Pneumonia and Respiratory Infections
- Autoimmune Bullous Skin Diseases
- Analytical Methods in Pharmaceuticals
- Protein purification and stability
- Lipid metabolism and biosynthesis
- Urticaria and Related Conditions
- Pancreatic function and diabetes
- HIV/AIDS drug development and treatment
- Advanced Proteomics Techniques and Applications
- Thyroid Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Software Reliability and Analysis Research
- Ubiquitin and proteasome pathways
- Computational Drug Discovery Methods
- Blood properties and coagulation
- Advanced Chemical Sensor Technologies
- Chemistry and Chemical Engineering
- Pneumocystis jirovecii pneumonia detection and treatment
- Reliability and Maintenance Optimization
- Macrophage Migration Inhibitory Factor
- Rheology and Fluid Dynamics Studies
- Metabolomics and Mass Spectrometry Studies
- Bacterial Infections and Vaccines
Merck & Co., Inc., Rahway, NJ, USA (United States)
2010-2025
University of Pittsburgh
1976-2024
East China University of Science and Technology
2016-2017
University of Colorado Denver
2007
University of Colorado Cancer Center
2007
University of North Carolina at Chapel Hill
2001
Medical College of Wisconsin
1997
Center for Rheumatology
1995
University of Michigan
1995
National Yang Ming Chiao Tung University
1993
The development of robust manufacturing processes for active pharmaceutical ingredients (APIs) is paramount to ensure a supply safe and effective medications. Implementation holistic control strategy, including quality incoming raw materials, key element in meeting this goal. This paper describes several examples from recent Merck API routes, which impurities materials affected the various ways, giving rise new process impurities, jeopardizing safety causing damage reaction vessels,...
Pemphigus vulgaris (PV) is a cutaneous autoimmune disease characterized by blister formation in the suprabasilar layers of skin and mucosae anti-desmoglein-3 (Dsg3) autoantibodies bound to surface lesional keratinocytes circulating serum patients. This can be reproduced neonatal mice passive transfer patients' IgG, indicating that humoral immunity plays an important role pathogenesis PV. Currently, T lymphocytes development PV not clear. Here, we report three immunoreactive segments...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT2,4-Diaminothieno[2,3-d]pyrimidines as antifolates and antimalarials. 2. Synthesis of 2,4-diaminopyrido[4',3':4,5]thieno[2,3-d]pyrimidines 2,4-diamino-8H-thiopyrano[4',3':4,5]thieno[2,3-d]pyrimidinesM. Chaykovsky, M. Lin, A. Rosowsky, E. J. ModestCite this: Med. Chem. 1973, 16, 3, 188–191Publication Date (Print):March 1, 1973Publication History Published online1 May 2002Published inissue 1 March...
Diacylglycerol acyltransferase 1 (DGAT1) presents itself as a potential therapeutic target for obesity and diabetes its important role in triglyceride biosynthesis. Herein we report the rational design of novel class DGAT1 inhibitors featuring benzomorpholine core (23n). SAR exploration yielded compounds with good potency selectivity well reasonable physical pharmacokinetic properties. This was tested rodent models to evaluate inhibition approach treatment metabolic diseases. Compound 23n...
MK-7845 was designed as a 3C-like protease inhibitor for the treatment of COVID-19. To enable rapid kilo-scale delivery to accelerate its First-in-Human studies, we developed fit-for-purpose process produce two key building blocks in less than months. The discoveries were highly diastereoselective Ellman addition route β-aminoamide 6 and crystallization isolation methods acid 9 with good quality control.
Autoantibodies associated with herpes gestationis (HG), a pregnancy-associated autoimmune skin disease, target the hemidesmosomal protein BP180. It was shown that major noncollagenous stretch of BP180 ectodomain (NC16A) harbors epitopes recognized by HG sera. Furthermore, Abs reactive homologous domain murine are known to trigger cutaneous blistering disease in mice passive transfer experiments. The present study aimed at characterizing T cell responses and specificities autoantibodies from...
Bacterial infection remains as one of the major healthcare issues, despite significant scientific and medical progress in this field. Infection by Streptococcus Pneumoniae (S. Pneumoniae) can cause pneumonia other serious infectious diseases, such bacteremia, sinusitis meningitis. The pneumococcal capsular polysaccharides (CPS) that constitute outermost layer bacterial cell are main immunogens protect pathogen from host defense mechanisms. Over 90 CPS serotypes have been identified, among...
We describe the rapid end-game process development for first good manufacturing (GMP) delivery of 3C-like protease inhibitor MK-7845 (1), an experimental treatment SARS-CoV-2. Three operations, including amide-coupling, oxidation, and crystallization, were rapidly developed implemented on a kilogram scale to enable critical safety studies phase 1 clinical trials move forward highly accelerated timeline. Key success this undertaking was our focus purging key impurities formed in...
Human gamma delta T cells expressing the V 9/V 2 T-cell receptor have been previously found to proliferate in response certain microorganisms and expand throughout life, presumably because of extrathymic activation by foreign antigens. In vitro expansion mycobacteria has shown be dependent on accessory cells. order gain an insight into mechanisms involved these cells, we undertaken identify peripheral blood subset which proliferation is dependent. Contrary their role antigen presentation...
Abstract Use of liquid chromatography/tandem mass spectrometric (LC/MS n ) molecular fingerprinting is systematically demonstrated as a very effective tool for rapid structural elucidation pharmaceutical impurities through case study in which three isomers betamethasone sodium phosphate (BSP) were rapidly identified degradants formed due to the D‐homoannular ring expansion steroid core structure BSP solid state. The these was achieved by matching or closely UV profiles, weights, and more...
7570 Background: TRIBUTE was a phase III, placebo-controlled study of patients with previously untreated advanced NSCLC. Patients received erlotinib (E) (150 mg/d) or placebo, plus course 6 cycles carboplatin and paclitaxel (CP), followed by maintenance E monotherapy (for those in the CP+E arm who were responding to treatment). 1,059 randomized treated (526 E; 533 placebo). There no significant differences OS, RR, TTP between two arms. In subgroup analyses pivotal 2nd line trial (BR21)...
Fibrinopeptide A (FPA) is a plasma peptide, formed by the action of thrombin on fibrinogen during clog formation. FPA represents direct indicator activity and could potentially be used as biomarker for anti-thrombotic therapy development. Results/Methodology: LC-MS/MS assay with high throughput solid phase extraction procedure was developed validated to measure in plasma. The lower limit-of-quantitation (LLOQ) this determined 0.16 nM. inter- intra-day%CV <15%. Freeze-thaw stability ±30% up 3...