A5083740583- Cholesterol and Lipid Metabolism
- Xenotransplantation and immune response
- Immune Cell Function and Interaction
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Fatty Acid Research and Health
- Animal Nutrition and Physiology
- Metabolomics and Mass Spectrometry Studies
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Animal Genetics and Reproduction
- Sphingolipid Metabolism and Signaling
- Platelet Disorders and Treatments
- Acute Myeloid Leukemia Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Protein Structure and Dynamics
- Endometrial and Cervical Cancer Treatments
- Gastrointestinal Tumor Research and Treatment
- Lipoproteins and Cardiovascular Health
- Gastric Cancer Management and Outcomes
- Pancreatic function and diabetes
- Hepatitis C virus research
- Ovarian cancer diagnosis and treatment
National Defense Medical College
2024
Tokyo National Hospital
2017-2018
Osaka University
1997-2014
Kokura Memorial Hospital
1995
University of Illinois Urbana-Champaign
1964-1993
National Hospital Organization Kochi National Hospital
1989
The University of Tokyo
1986
Nagasaki University
1986
University of Alabama at Birmingham
1985
Tohoku University
1979-1983
Phospholipid transfer protein (PLTP) was purified from lipoprotein-free human plasma, obtained upon treatment of plasma with dextran sulfate and Ca2+, by employing a series column chromatography. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the PLTP showed single main band, corresponding to molecular mass 78 kDa. However, isoelectric focusing preparation gave multiple bands pI ranging 4.3 5.1, indicative microheterogeneity. Purified shown possess not only phospholipid...
Cerebellar disorders developed in chicks 1-5 hours after intravenous injection of 10 mg the hydroperoxide methyl linoleate or linoleic acid emulsified 1 ml serum. No cerebellar were noted, injection, birds kept on an identical diet but supplemented with 8 % α-tocopherol. Injection reduced hydroperoxide, 12-oxo-cis-9-octadecenoate fresh caused no indicating only lipohydroperoxide initiated symptoms. The severity induced by was greatly influenced dietary pretreatment. cerebellum which had been...
The present results indicate that dietary protein tends to depress the atherogenic effect of cholesterol and fat. substitution heated oil for fresh depresses serum β-lipoprotein levels, but incidence atherosclerosis is at least as high with oil, indicating levels are not necessarily proportional degree experimental atherosclerosis.
The four peptide analogs of the amphipathic helix whose interactions with dimyristoyl phosphatidylcholine were described in preceding paper compared apolipoproteins (apo) A-I and A-II ability to displace native apolipoprotein from high density lipoprotein (HDL) activate lecithin:cholesterol acyltransferase. rank order apo-A-I intact HDL was 18A-Pro-18A greater than 18A des-Val10-18A reverse-18A, same suggested for relative lipid affinities. Modified which 40% had been displaced by...
Cytokeratins (CKs) are structural marker proteins specific for epithelial cells. However, recent studies indicate their involvement in cancer progression. We evaluated CK18 and its filament partner, CK8 expression, by immunohistochemistry 210 resected specimens from patients with oesophageal squamous cell carcinoma (OSCC). also analysed the relationship between expression various clinicopathological parameters including prognosis. Neither nor was expressed non-cancerous epithelium whereas...
Abstract A method for the purification of lipid transfer protein (LTP) from human plasma was developed with aid succinylated low density lipoprotein-Sepharose affinity column chromatography. The purified LTP exhibited a single main band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, upon isoelectric focusing polyacrylamide gel, preparations consistently showed nine bands points ranging 4.6 to 5.4. treatment Clostridium perfringens neuraminidase shifted these multiple...
Summary The nutritive value of polymerized fats was compared by feeding them at a 10% level to weanling rats kept on three different basal rations and various vitamin supplements. Autoxidatively or thermally autoxidatively depressed growth, were toxic rats. results indicated that the dietary riboflavin pyridoxine manner in which had an important bearing their nutritional value. protein also influenced oils and, lower levels, appeared be related intake as growth depression toxicity partially...
The relationship between the cholesterol ester (CE) transfer activity of lipid protein (LTP) and its affinity with lipoprotein particles was investigated. study effects chemical modification low density (LDL) amino groups carboxyl on CE showed that maximal is obtained upon succinylation or acetylation approximately 7% LDL groups. Further increases in extent progressively reduced activity. treatment fatty acids gave results comparable to addition concentrations stimulatory, while high...
Lecithin and lecithin/cholesterol dispersions as well lysolecithin micelles were used to provide basic information on the mechanism of interaction zwitterionic phospholipids with dextran sulfate. The addition sulfate lecithin or in presence Ca2+ produced insoluble complexes. At each concentration, amount complex formed was maximal at equivalence sulfate/phospholipid weight ratio. An increase CaCl2 concentration up 10 mM progressively increased ratios for phospholipids. Further did not...
The effects of human apolipoproteins on the lecithin-cholesterol acyltransferase reaction were studied by using purified and phosphatidylcholine-cholesterol vesicles. When assay mixtures contained an optimal amount or excess apo-A-I, addition apo-A-II, apo-C-II, apo-C-III1, apo-C-III2 inhibited enzymatic reaction. However, at suboptimal apo-A-I concentrations, low concentrations these exhibited activating effects. relative greater lower levels. Under no circumstance did combined effect other...
The abbreviations used are: apo, apolipoprotein; HDL, high density lipoproteins; LDL, low VLDL, very lipoproteins.matic reaction may require the simultaneous presence of apo-A-I and enzyme on substrate particles, no strong interaction appears to exist between
The effects of lecithin-cholesterol acyltransferase (LCAT) on the transfer cholesterol esters mediated by lipid protein (LTP) and its affinity for lipoprotein particles were investigated. When single bilayer vesicle preparations (containing phosphatidylcholine, cholesterol, cholesteryl ester, apolipoprotein- (apo) A-I at molar ratio 90:30:1.2:0.18) or high density 3 (HDL3) used as ester donor low lipoproteins (LDL) acceptor, activity LTP was enhanced addition concentrations LCAT. In...
In small bowel transplantation (SBTx), graft-versus-host disease (GVHD) is mediated by donor-derived T cells recognizing host major histocompatibility complex (MHC) alloantigens, and represents an important immunological event influencing life in experimental clinical situations. We evaluated the possibility that a new sphingosine 1-phosphate receptor agonist, FTY720, could diminish GVHD rat SBTx model through traffic alteration of target organs. Heterotopic was performed using parent...
The present study has revealed that 45% of acid phosphatase in rat poly-morphonuclear leucocytes can be recovered from the granular fraction, 21% nuclear and 34% supernatant fraction. Acid granule fraction appeared to have properties distinctly different those enzyme soluble fractions. was more stable, possessed a lower pH optimum, preferentially inhibited by sodium fluoride.