A5009421046- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Protein purification and stability
- Metabolomics and Mass Spectrometry Studies
- Nutrition and Health in Aging
- Pesticide Residue Analysis and Safety
- Kidney Stones and Urolithiasis Treatments
- Liver Disease Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Cytokine Signaling Pathways and Interactions
- Biosensors and Analytical Detection
- Muscle metabolism and nutrition
- Cardiac electrophysiology and arrhythmias
- Cholesterol and Lipid Metabolism
- Fibroblast Growth Factor Research
- Lipoproteins and Cardiovascular Health
- Parathyroid Disorders and Treatments
- Analytical chemistry methods development
- Biochemical effects in animals
- Fermentation and Sensory Analysis
- Genetic and Kidney Cyst Diseases
- Transgenic Plants and Applications
- Fungal and yeast genetics research
- Plant Virus Research Studies
- Urological Disorders and Treatments
Wenzhou University
2019-2021
University of Alberta
2011-2016
Wenzhou Medical University
2015
Stanford University
2006-2008
VA Palo Alto Health Care System
2006-2008
China Medical University
2006
Hamamatsu University School of Medicine
2004
We report an improved shotgun method for analyzing proteomic samples containing sodium dodecyl sulfate (SDS). This is based on the use of strong-cation exchange (SCX) liquid chromatography (LC) SDS removal that can be integrated with peptide separation as first dimension two-dimensional LC tandem mass spectrometry workflow. To optimize performance removal, various experimental conditions, including concentrations chemical reagents and salts in sample, concentration, SCX mobile phase...
Gram-negative sepsis with release of endotoxin is a frequent cause cachexia that develops partly because resistance to growth hormone (GH) reduced insulin-like factor-I (IGF-I) expression. We set out more fully characterize the mechanisms for and determine whether in addition defect janus kinase 2 (JAK2)-signal transducer activator transcription (STAT) 5b pathway, required GH-induced IGF-I expression, there might also be distal defect. Conscious rats were given studied 4 h later. In liver...
Information about how yeast metabolism is rewired in response to internal and external cues can inform the development of metabolic engineering strategies for food, fuel, chemical production this organism. We report a new metabolomics workflow characterization such rewiring. The combines efficient cell lysis without using chemicals that may interfere with downstream sample analysis differential isotope labeling liquid chromatography mass spectrometry (CIL LC–MS) in-depth metabolome...
Low‐density lipoprotein receptor (LDLR) catalyzes the uptake of LDL‐cholesterol by liver and peripheral organs. The function LDLR is antagonized pro‐protein convertase subtilisin/kexin type 9 (PCSK9), which binds to at plasma membrane inducing degradation. Here, we report that matrix metalloproteinase‐2 (MMP‐2) interacts with cleaves PCSK9, as evidenced proteomic, chemical cross‐linkage, blue native‐PAGE domain‐specific antibodies Western blot analyses. Furthermore, MMP‐2 overexpression...
Growth hormone (GH) is required to maintain normal cardiac structure and function has a positive effect on remodeling in experimental possibly human disease. Cardiac resistance GH develops the uremic state, perhaps predisposing characteristic cardiomyopathy associated with uremia. It was hypothesized that administration of low-dosage may have salutary process uremia, but because high levels adverse effects, high-dosage worsen cardiomyopathy. In rats chronic renal failure, quantitative...
We report an enabling method for mapping the protein sequence with high coverage. This combines separation power of gel electrophoresis coverage capability microwave-assisted acid hydrolysis (MAAH) mass spectrometry (MS). In-gel MAAH using 25% trifluoroacetic was developed and optimized degrading gel-separated into small peptides suitable tandem MS sequencing. For bovine serum albumin (BSA) (∼67 kDa), 4 μg loading onto a separation, followed by excising band in-gel then injecting ∼2...
Podocytes are essential components of the glomerular basement membrane. Epithelial-mesenchymal-transition (EMT) in podocytes results proteinuria. Fibroblast growth factor 1 (FGF1) protects renal function against diabetic nephropathy (DN). In present study, we showed that treatment with an FGF1 variant decreased mitogenic potency (FGF1 ΔHBS ) inhibited podocyte EMT, depletion, fibrosis, and preserved two models. Mechanistic studies revealed inhibitory effects EMT were mediated by expression...
Introduction: The objective of the present study was to investigate influence cigarette smoking on serum and cerebrospinal fluid (CSF) calcium levels in Chinese heavy smokers.Methods: One hundred sixty-seven male subjects were divided into non-smokers (95) smokers (72). Serum CSF concentrations assayed. Smoking status recorded degree nicotine dependence (FNTD) assessed by questionnaire. Analysis variance performed assess differences among dichotomous groups. Partial correlations between...