A5065330974- CRISPR and Genetic Engineering
- Cerebrovascular and genetic disorders
- Metalloenzymes and iron-sulfur proteins
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Barrier Structure and Function Studies
- Neurogenesis and neuroplasticity mechanisms
- Innovation and Socioeconomic Development
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Virus-based gene therapy research
- Insect symbiosis and bacterial influences
- Renal and related cancers
- Hedgehog Signaling Pathway Studies
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- CAR-T cell therapy research
Korea Research Institute of Bioscience and Biotechnology
2019-2024
University of Alabama at Birmingham
2024
Korea University of Science and Technology
2019-2022
Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured cells lack capacity to interact with other somatic cell types, which is distinct from developing vascular in vivo. Recently, it was demonstrated that blood vessel (BVOs) recreate structure functions human vessels. tissue-specific adaptability BVOs had not assessed tissues. Herein, we investigated whether infiltrate cerebral form a blood–brain barrier. As result,...
Abstract CRISPR effectors, which comprise a CRISPR-Cas protein and guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When gRNA recognizes genomic loci with sequences that similar to target, deleterious mutations can occur. Off-target frequency below 0.5% remain mostly undetected by current genome-wide off-target detection techniques. Here we report method effectively detect extremely small amounts of mutated DNA based on predicted...
Abstract Human blood vessel organoids (hBVOs) offer a promising platform for investigating vascular diseases and identifying therapeutic targets. In this study, we focused on in vitro modeling target finding of cerebral autosomal dominant arteriopathy with subcortical infarcts leukoencephalopathy (CADASIL), the most common form hereditary stroke disorder caused by mutations NOTCH3 gene. Despite identification these mutations, underlying pathological mechanism is elusive, effective approaches...
Abstract CRISPR effectors, which comprise a CRISPR-Cas protein and guide (g)RNA derived from the bacterial immune system, are widely used to induce double-strand breaks in target DNA activate in-vivo repair system for target-specific genome editing. When gRNA recognizes genomic loci with sequences that similar target, deleterious often carcinogenic mutations can occur. Off-target frequency below 0.5% remain mostly undetected by current genome-wide off-target detection techniques. In this...
Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount evidences had implicated hormones and hormone-like molecules as critical regulators proliferation lineage specification during vivo development. Therefore, a deeper understanding the involved cell fate decisions efficient controlled hPSCs lineages. Thus, we functionally quantitatively compared effects diverse (estradiol 17-β (E2),...
Abstract Human blood vessel organoids (hBVOs) offer a promising platform for investigating vascular diseases and identifying therapeutic targets. In this study, we focused on in vitro modeling target finding of cerebral autosomal dominant arteriopathy with subcortical infarcts leukoencephalopathy (CADASIL), the most common form hereditary stroke disorder caused by mutations Notch3 gene. Despite identification these mutations, underlying pathological mechanism is elusive, effective approaches...
Universally acceptable donor cells have been developed to address the unmet need for immunotypically matched materials regenerative medicine. Since forced expression of hypoimmunogenic genes represses immune response, we established universal pluripotent stem (PSCs) by replacing endogenous β2-microglobulin (β2m) with β2m directly conjugated human leukocyte antigen (HLA)-G, thereby simultaneously suppressing HLA-I and natural killer (NK) cell-mediated response. These modified PSCs retained...