A5068697669- Neuroinflammation and Neurodegeneration Mechanisms
- Electromagnetic Fields and Biological Effects
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Parkinson's Disease Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- bioluminescence and chemiluminescence research
- Protease and Inhibitor Mechanisms
- RNA and protein synthesis mechanisms
- Neurological disorders and treatments
- Advanced Neuroimaging Techniques and Applications
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- DNA Repair Mechanisms
- Alzheimer's disease research and treatments
- Animal Genetics and Reproduction
- Microtubule and mitosis dynamics
- Renal and related cancers
- Stress Responses and Cortisol
- Heat shock proteins research
- Cancer-related Molecular Pathways
- Innovation and Socioeconomic Development
Gyeongsang National University
2009-2025
Korea Research Institute of Bioscience and Biotechnology
2014-2023
Institute of Primate Research
2017
Ewha Womans University
2011-2015
Korea Institute of Radiological and Medical Sciences
2010-2014
Korea Atomic Energy Research Institute
2013
Hanyang University
2012
Radiation Effects Research Foundation
2010-2011
SIRT3 (sirtuin 3), a mitochondrial protein deacetylase, maintains respiratory function, but its role in the regulation of innate immune defense is largely unknown. Herein, we show that coordinates function and macroautophagy/autophagy activation to promote anti-mycobacterial responses through PPARA (peroxisome proliferator activated receptor alpha). deficiency enhanced inflammatory dysfunction, leading defective host pathological inflammation during mycobacterial infection. Antibody-mediated...
Abstract The CRISPR–Cas9 system is widely used for target-specific genome engineering. CRISPR–Cas12a (Cpf1) one of the CRISPR effectors that controls target genes by recognizing thymine-rich protospacer adjacent motif (PAM) sequences. Cas12a has a higher sensitivity to mismatches in guide RNA than does Cas9; therefore, off-target sequence recognition and cleavage are lower. However, it tolerates regions distant from PAM (TTTN or TTN) protospacer, issues may become more problematic when...
Abstract CRISPR effectors, which comprise a CRISPR-Cas protein and guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When gRNA recognizes genomic loci with sequences that similar to target, deleterious mutations can occur. Off-target frequency below 0.5% remain mostly undetected by current genome-wide off-target detection techniques. Here we report method effectively detect extremely small amounts of mutated DNA based on predicted...
Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters cynomolgus and rhesus monkeys limited. In the present study, we established hematologic biochemical intervals healthy anesthetized with ketamine hydrochloride. A total of 142 (28 males 114 females) 42 (22 20 were selected analyzed order to examine hematological 16 parameters. The effects sex also investigated. Reference separately by species (cynomolgus rhesus) (male...
X-box binding protein 1 (XBP1) mRNA processing plays a crucial role in the unfolded response (UPR), which is activated to endoplasmic reticulum (ER) stress. Upon accumulation of UPR-converted XBP1 splicing from an unspliced (u) (inactive) isoform spliced (s) (active) isoform, inositol-requiring enzyme α (IRE1α) removes 26-nucleotide intron uXBP1 mRNA. Recent studies have reported assessment ER stress by examining ratio sXBP1 (s/uXBP1 ratio) via densitometric analysis PCR bands relative...
Purpose: Radiation-induced cardiovascular disease is a potentially severe side-effect of thoracic radiotherapy treatment. Clinically, this delayed presents as form accelerated atherosclerosis several years after irradiation. As general endothelial dysfunction known to be an initiating event in radiation-induced vascular damage, we examined the effects radiation on cells atherosclerosis.
In line with recent findings showing Alzheimer's disease (AD) as an insulin-resistant brain state, a non-transgenic animal model intracerebroventricular streptozotocin (icv-STZ) administration has been proposed representative experimental of AD. Although icv-STZ rodent models AD have increasingly researched, studies in non-human primate are very limited. this study, we aimed to characterize the cerebral damage caused by primates; achieve this, three cynomolgus monkeys (Macaca fascicularis)...
Abstract Several groups have reported that TGFβ1 regulates cellular responses to γ-irradiation; however, the exact mechanism has not been fully elucidated. In current study, role of in γ-irradiation was investigated detail. The data indicate pretreatment decreased aftermath ionizing radiation (IR)-induced DNA damage a SMAD-dependent manner. To determine underlying for these effects, extent IR-induced repair activity presence or absence examined. Studies reveal upregulated ligase IV (Lig4),...
Mitochondria continuously fuse and divide to maintain homeostasis. An impairment in the balance between fusion fission processes can trigger mitochondrial dysfunction. Accumulating evidence suggests that dysfunction is related neurodegenerative diseases such as Parkinson's disease (PD), with excessive dopaminergic neurons being one of pathological mechanisms PD. Here, we investigated substantia nigra a non-human primate model 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced We...
Background: The emergence of new psychoactive substances (NPSs) poses a serious global health threat. Although various groups psychostimulants exist, this study specifically investigated two lesser-studied substances, 4,4'-dimethylaminorex (4,4'-DMAR) and escaline.Objective: To assess liability to substance use disorder (SUD), as evidenced via preclinical models, the psychostimulants.Methods: 4,4'-DMAR escaline were evaluated, in mice, for their potential exhibit rewarding reinforcing...
Abstract Background Maladaptive decision-making by methamphetamine users may reflect circuit-level dysfunction underlying deficits in neural activity. In a previous study, it was reported that heightened resting-state connectivity within the mesocorticolimbic system, coupled with reduced prefrontal cortical connectivity, created bias toward reward-driven behavior over cognitive control users. Aim & Objectives The primary aim of this study to investigate effect chronic administration on...
Abnormal epigenetic reprogramming of nuclear-transferred (NT) embryos leads to the limited efficiency producing cloned animals. Trichostatin A (TSA), a histone deacetylase inhibitor, improves NT embryo development, but its role in acetylation porcine with mesenchymal stem cells (MSCs) is not fully understood. This study aimed compare effects TSA on patterns, and key epigenetic-related genes between vitro fertilization (IVF), NT-MSC, 40 nM TSA-treated NT-MSC (T-NT-MSC). The results...
Abstract Wireless mobile phones and other telecommunication devices are used extensively in daily life. We therefore examined the effects of combined exposure to radiofrequency electromagnetic fields (RF‐EMF) on rat testicular function, specifically with respect sensitive processes such as spermatogenesis. Male rats were exposed single code division multiple access (CDMA) wideband (WCDMA) RF signals for 12 weeks. The schedule comprised 45 min/day, 5 days/week a total whole‐body average...
The guidelines for applying individual adjustments to macaques according the severity of behavioral symptoms during 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment were provided reproduce stable chronic Parkinsonism in a recent study (Potts et al., 2014). But, since there are insufficient regarding objective criteria MPTP treatment, it is difficult develop MPTP-induced non-human primate (NHP) models with symptoms.The administration based on results automatic quantification...
Hyeon-Gu Yeo, Jung Joo Hong, Youngjeon Lee, Kyung Sik Yi, Chang-Yeop Jeon, Junghyung Park, Jinyoung Won, Jincheol Seo, Yu-Jin Ahn, Keonwoo Kim, Seung Ho Baek, Eun-Ha Hwang, Green Yeung Bae Jin, Kang-Jin Jeong, Bon-Sang Koo, Philyong Kang, Seob Lim, Sun-Uk Jae-Won Huh, Young-Hyun Yeonghoon Son, Ji-Su Chi-Hoon Choi, Sang-Hoon Cha and Sang-Rae Lee. Exp Neurobiol 2019;28:458-73. https://doi.org/10.5607/en.2019.28.4.458
Study of interactions between the nervous system and immunity offers insights into pathogenesis Parkinson's disease (PD) potential therapeutic strategies for neurodegenerative diseases. Studies on rodents have revealed regulatory mechanisms microglial activation T lymphocyte recruitment in PD. However, underlying chronic infiltration brain after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injection a non-human primate (NHP) model PD remain unknown. This study aimed to investigate...
Abstract Background Ischemic stroke is a serious neurological disorder caused by blockages in cerebral artery. Protein phosphatase 2A (PP2A) that performs critical role cell signaling and growth. PP2A subunit B acts as neuroprotective agent the nerve system. Chlorogenic acid, which mainly found roasted coffee, has antioxidant, anti-inflammatory, anti-apoptotic effects. We hypothesized chlorogenic acid modulates expression ischemic models glutamate-mediated neurons. Middle artery occlusion...