The small intestine is not only critical for nutrient absorption, but also serves as an important immune organ. Medium-chain fatty acids have nutritional and metabolic effects support the integrity of intestinal epithelium. However, their roles in immunity pigs are fully understood. We investigated a medium-chain acid, capric on oxidative stress, inflammation, barrier function porcine epithelial cells miniature after treatment with suppressant cyclophosphamide. Capric acid alleviated...

Abstract The gastrointestinal epithelium functions in nutrient absorption and pathogens barrier its dysfunction directly affects livestock performance. N-Acetylcysteine (NAC) improves mucosal function, but effects on intestinal at the molecular level remain unclear. Here, we performed gene expression profiling of pig small intestine after dietary NAC treatment under LPS challenge investigated epithelial cells vitro . Dietary supplementation altered 959 genes related to immune response,...

In most animals, primordial germ cells (PGCs) originate from an extragonadal region and migrate across the embryo to gonads, where they differentiate function. During their migration, PGCs move passively by morphogenetic movement of or actively through signaling molecules. To uncover underlying mechanism first-phase PGC migration toward germinal crescent in chickens, we investigated spatial temporal action during primitive streak formation. Exogenously transplanted migrated anterior...

In the poultry industry, most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate regulatory pathways during muscle differentiation. To gain insight of differentiation mechanism growth period, we identified validated calcium-related genes which highly expressed through mRNA sequencing analysis.We next-generation-sequencing (NGS) analysis from undifferentiated QM7 cells differentiated (day 1 day 3 periods). Subsequently, obtained...

Abstract Immunosuppression directly correlates with economic benefits in livestock. Although omega-3, known as an energy source, is used a pharmaceutical molecule, it remains unknown whether dietary supplementation omega-3 can alleviate cyclophosphamide-induced immunosuppression pigs. Omega-3 treatment increased the number of white blood cell, lymphocytes, and monocytes decreased tumor necrosis factor (TNF)-α production under CTX challenge. In addition, we confirmed that expression nuclear...

Deoxynivalenol (DON) is a mycotoxin that found in feed ingredients derived from grains such as corn and wheat. Consumption of DON-contaminated has been shown to cause damage the intestine, kidneys, liver. However, molecular mechanism by which DON exerts its effect small intestine not completely understood. As result, we profiled gene expression intestinal epithelial cells treated with examined function vitro. We hypothesized could induce apoptosis via FOXO3a-signaling pathway based on these...

The influenza A virus infects a broad range of species and spreads easily through the respiratory tract. Because these characteristics, has caused pandemic disease in humans livestock. To investigate early molecular responses after infection chickens, we infected tracheal epithelial cells derived from 20-day-old chick embryos with (H1N1). gene expression patterns were analyzed via DNA microarray at different time points (0, 6, 12, 24, 36 hr) viral infection. Differentially expressed genes...

Cyclophosphamide, a cytotoxic anticancer agent, induces immunosuppression and has several adverse effects. N-acetylcysteine alleviates oxidative stress, liver injury, intestinal tissue damage. The present study examined whether modulates the effects of cyclophosphamide in pigs. Miniature pigs (n = 15) were used as an experimental model to evaluate treatment on immune reactions, stress after challenge. Corn-soybean meal based dietary treatments follows: control diet with either saline...

1. In the poultry industry, growth performance is important due to its effects on economic value. Much effort has been put forth achieve introgression of specific genes and DNA markers related muscle proliferation differentiation in selective breeding approaches. 2. This study investigated biological functions gene Forkhead box O3 (FOXO3) during myogenic chicken myoblast cells. FOXO3 was downregulated primary (pCM) cells by piggyBac transposon-mediated microRNA (miRNA) knock-down (KD)...

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, which is the most efficient and reliable tool for precisely targeted modification of genome living cells, has generated considerable excitement industrial applications as well scientific research. In this study, we developed a gene-editing detection system chick embryo sexing during embryonic stage.By combining CRISPR/Cas9 technical platform germ cell-mediated germline...

Intracytoplasmic sperm injection (ICSI) is an important technique in animal biotechnology for cloning and conservation of genetic resources, but has been a challenge avian species. In the present study, we investigated ability cryopreserved quail spermatozoa to achieve fertilisation embryo development. Female were killed 70-120min after previous oviposition collect unfertilised oocytes from oviduct. Fresh or cryopreserved-thawed injected into cytoplasm oocytes, manipulated incubated...

The porcine immune system has an important role in pre-clinical studies together with understanding the biological response mechanisms before entering into clinical trials. size distribution of Korean minipig is feature that make this breed ideal for biomedical research and safe practice post studies. extremely tiny (ET) serves as excellent model various studies, but comparatively frail vulnerable to environment over its Large (L) leads additional after born care. To overcome pitfall,...

Functional dyspepsia (FD) is a gastrointestinal disorder with diverse symptoms but no structural or organic manifestations.Benachio-F ® (herein named 'BF-1') an over-the-counter liquid digestive formulated multiple herbal extracts, which has been reported to improve of FD.A total two experiments were conducted.First, we examined whether BF-1 can modulate the progression FD through experimental rat models.A three doses (0.3x, 1x, 3x human equivalent dose) used.In gastric emptying model, both...

The avian embryos have been used as a good model to study embryonic development. Due its unique development in the eggshell, can be cultured and hatch surrogate eggshell system. In this study, we examined viability, normal hatchability of Korean Oge (KO) chicken White Leghorn (WL) eggshells. Donor KO at 3-day 4-day-old were transferred into recipient WL eggshells, incubated for further 18 days <TEX>$37.5^{\circ}C$</TEX> with 70% humidity until hatching. viability 3-day-old 7, 14 21 day...

Eggshell Membrane (ESM) has been used as an alternative natural bandage to cure wounds and is available in large quantities from egg industries. This study based on the use of resources for skin tissue reconstruction. It needs understand regeneration with Extracellular Matrix (ECM). Wound healing a complicated continuous recovery process damaged tissues by replacing dysfunctional injured cellular structures. The wound recruits some different phases: first phase hemostasis, second...

As the trade among countries increases, domestic agriculture is facing a crisis. Especially rice industry suffering from market opening problem and itself. counter measure against this, despite promotion of processing food industry, export processed has decreased by an average annual rate 12.3% since 2011 due to lack customized strategy, such as low price competitiveness, insufficient quality brand management. measure, we selected indicators prioritize establishment strategy business used...