A5041890823- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Dark Matter and Cosmic Phenomena
- Particle physics theoretical and experimental studies
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Atomic and Subatomic Physics Research
- Advancements in Semiconductor Devices and Circuit Design
- Neutrino Physics Research
- RNA Interference and Gene Delivery
- Semiconductor materials and devices
- MicroRNA in disease regulation
- Astrophysics and Cosmic Phenomena
- Virus-based gene therapy research
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Nanowire Synthesis and Applications
- Lipid metabolism and biosynthesis
- RNA and protein synthesis mechanisms
- Nuclear Physics and Applications
- PI3K/AKT/mTOR signaling in cancer
- Advanced Data Storage Technologies
- Radio Frequency Integrated Circuit Design
- GDF15 and Related Biomarkers
University of Wisconsin–Madison
2022-2024
University of Wisconsin Carbone Cancer Center
2022-2024
Seoul National University
2006-2021
Seoul National University of Science and Technology
2016-2020
Kyungpook National University
2008-2019
Ewha Womans University
2007-2008
Tsinghua University
2007-2008
Sejong University
2008
Yonsei University
2003
The Korea Invisible Mass Search (KIMS) experiment presents new limits on the weakly interacting massive particle (WIMP)-nucleon cross section using data from an exposure of 3409 kg·d taken with low-background CsI(Tl) crystals at Yangyang Underground Laboratory. most stringent limit spin-dependent interaction for a pure proton case is obtained. DAMA signal region both spin-independent and interactions WIMP masses greater than 20 GeV/c2 excluded by single crystal scintillators.Received 3 April...
Many studies have been conducted to improve economically important livestock traits such as feed efficiency and muscle growth. Genome editing technologies represent a major advancement for both basic research agronomic biotechnology development. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technical platform is powerful tool used engineer specific targeted loci. However, the potential occurrence of off-target effects, including cleavage unintended targets,...
Search for neutrino-less double beta decay of <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">100</sup> Mo is proposed using active method with Ca MoO <sub xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> scintillation crystals which show the brightest light among variety inorganic materials containing Mo. Study X-ray luminescence and properties such as energy response, number photoelectrons/keV, absolute yield, time, pulse shape...
ABSTRACT Chicken as a food source is one of the most widespread domestic animals, and it has been used extensively research model. The clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated protein 9 (Cas9) system efficient reliable tool for precise genome‐targeted modification generated considerable excitement industrial applications, well biologic science. Unlike in mammals, germline‐transmittable primordial germ cells (PGCs) chicken were an alternative...
We present a search for low-mass ($\leq 20 GeV/c^{2}$) weakly interacting massive particles(WIMPs), strong candidates of dark matter particles,using the low-background CsI(Tl) detector array Korea Invisible Mass Search (KIMS) experiment. With total data exposure 24,324.3kg$\cdot$days,we WIMP interaction signals produced by nuclei recoiling from WIMP-nuclear elastic scattering with visible energies between 2 and 4keV. The observed energy distribution candidate events is consistent null...
In the poultry industry, most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate regulatory pathways during muscle differentiation. To gain insight of differentiation mechanism growth period, we identified validated calcium-related genes which highly expressed through mRNA sequencing analysis.We next-generation-sequencing (NGS) analysis from undifferentiated QM7 cells differentiated (day 1 day 3 periods). Subsequently, obtained...
Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement economic traits in livestock as well poultry. In this study, we examined and verified nickase mutated CRISPR-associated protein 9 (Cas9) modulate specific target gene chicken DF1 cells. Chicken myostatin which inhibits muscle cell growth differentiation during myogenesis was targeted be deleted by Cas9-D10A nickase. After co-transfection expression...
Abstract Background In the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Thus, functional genomic studies help to generate faster, healthier, more efficient animal production. Chicken myoblast cells, which are required muscle development regeneration, particularly important because chicken growth closely related mass. Results this study, we induced expression of microRNA-146b-5p mediated by piggyBac transposon system in primary (pCM)...
In the livestock industry, regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth meat production. We investigated pathway MyoD its role in quail myoblast cells. The gene was mutated by clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology single cell-derived mutant sublines were identified investigate global mechanism responsible for differentiation. mutation efficiency 73.3% mixed population, from this...
In mammals, germ cells originate outside of the developing gonads and follow a unique migration pattern through embryonic tissue toward genital ridges. Many studies have attempted to identify critical receptors factors involved in cell migration. However, relatively few reports exist on chemokines that are avian species. present study, we investigated specific migratory function C-X-C chemokine receptor type 4 (CXCR4) chicken primordial (PGCs). We induced loss-of-function via frameshift...
Objective Owing to the public availability of complete genome sequences, including avian species, massive bioinformatics analyses may be conducted for computational gene prediction and identification regulatory networks through various informatics tools. However, evaluate biofunctional activity a predicted target gene, in vivo vitro functional genomic should prerequisite. Methods Due lack quail sequence information, we first identified partial structure sequences SH3 domain containing ring...
1. In the poultry industry, growth performance is important due to its effects on economic value. Much effort has been put forth achieve introgression of specific genes and DNA markers related muscle proliferation differentiation in selective breeding approaches. 2. This study investigated biological functions gene Forkhead box O3 (FOXO3) during myogenic chicken myoblast cells. FOXO3 was downregulated primary (pCM) cells by piggyBac transposon-mediated microRNA (miRNA) knock-down (KD)...
Transgenic technology is widely used for industrial applications and basic research. Systems that allow genetic modification play a crucial role in biotechnology number of purposes, including the functional analysis specific genes production exogenous proteins. In this study, we examined verified cumate-inducible transgene expression system chicken DF1 quail QM7 cells, as well loxP element-mediated recombination using Cre recombinase cells.After stable transfer with piggyBac transposon...
Since athletic performance is a most importance trait in horses, research focused on physiological and physical studies of horse abilities. In contrast, the molecular analysis as well regulatory pathway remain insufficient for evaluation prediction our previous study, we identified AXL receptor tyrosine kinase (AXL) gene which was expressed alternative spliced isoforms skeletal muscle during exercise. present validated two splicing transcripts (named AXLa long form AXLb short form) equine to...
Phosphoinositide (PIPn) signaling plays pivotal roles in myriad biological processes and is altered many diseases including cancer. Canonical PIPn involves membrane-associated lipid second messengers that modulate protein recruitment activity at membrane focal points. In the nucleus, operates separately from membranous compartments defining paradigm of non-canonical signaling. However, mechanisms by which this non-membranous nuclear pool established mediates stress poorly understood. The...
Investigation of 4-bit SONOS nonvolatile memory with 3-dimentional structure has been done using a 3- dimensional numerical simulation tool. The impact channel length and the interference stored charge on opposite side are observed by changing Sifin width. It is estimated that device can be scaled down to gate length/fin width 70/30 nm sufficient V <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">TH</inf> window margin 2 V.
A bioreactor can be used for mass production of therapeutic proteins and other bioactive substances. Although various methods have been developed using microorganisms animal cells, advanced strategies are needed the efficient biofunctional proteins. In microorganisms, post-translational glycosylation modification not performed properly, while cell systems require more time expense. To overcome these problems, new products from transgenic animals considered, such as genetically modified cow’s...
A detector for space-time block coding is proposed to combat time-selective fading. To suppress both noise and interference, a minimum mean square error (MMSE) based introduced coding. It shown by simulations that the outperforms conventional detectors when channel
We developed a SrCl <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> crystal scintillator for 0υ Electron Capture/β+ decay search, and 4π CsI(Tl) tagging detector to reduce the background. This experimental system was installed at YangYang underground laboratory which is WIMP search by KIMS group. The ran 60 days we have finished analyzing 2 hits back coincidence signal selection.