Aim: To improve the diagnostic accuracy of immuno-PCR (I-PCR) in tuberculosis (TB) patients by using functionalized gold nanoparticles (AuNPs) coupled with detection antibodies and oligonucleotides, magnetic beads (MBs) conjugated capture liquid phase. Materials & methods: MB-coupled AuNP-based I-PCR (MB-AuNP-I-PCR) assay was designed to detect a cocktail Mycobacterium MPT64 CFP-10 proteins bodily fluids TB patients. Results: The sensitivities 89.3 (n = 94) 78.1% 73) were observed pulmonary...

The current generation is looking for new materials and technology to reduce the dependency on fossil fuels, exploring sustainable energy sources maintain future demand supply.

Diagnosis of extrapulmonary tuberculosis (EPTB) poses serious challenges. A careful selection appropriate gene targets is essential for designing a multiplex-polymerase chain reaction (M-PCR) assay. We compared several Mycobacterium tuberculosis, including IS6110, devR, and genes encoding MPB-64 (mpb64), 38kDa (pstS1), 65kDa (hsp65), 30kDa (fbpB), ESAT-6 (esat6), CFP-10 (cfp10) proteins, using PCR assays on 105 EPTB specimens. From these data, we chose the two best to design an M-PCR. Among...

Aim: Diagnosis of osteoarticular tuberculosis (OATB) is quite challenging and there an urgent need to design a prompt precise diagnostic test. Methods: We developed multi-targeted loop-mediated isothermal amplification (LAMP) assay using mpt64 (Rv1980c) pstS1 (Rv0934) targets for the detection Mycobacterium in OATB patients. Results: The sensitivities 100 82.4% were obtained confirmed (n = 10) suspected 57) cases, respectively by LAMP with specificity 96.9% 33). Moreover, attained total...

The present study aimed to develop enteric-coated mucoadhesive pellets of Tofacitinib citrate (TOF) for site-specific delivery in the treatment ulcerative colitis (UC). formulation (B2) was selected based on uniform particle size distribution, flow properties, and drug content. Further, it showed robust mechanical characteristics minimized friability. FTIR studies revealed purity TOF good compatibility with excipients. Meanwhile, DSC XRD demonstrated successful confinement reduced...

Aim: There is an urgent need to design a reliable diagnostic test for tuberculosis (TB). Methods: Real-time immuno-PCR (RT-I-PCR) assay was devised the quantitative detection of cocktail mycobacterial MPT64 (Rv1980c) and PstS1 (Rv0934) in TB patients. Results: A broad dynamic range 0.95 pg/ml–95 ng/ml MPT64+PstS1 detected In smear-positive (n = 59) smear-negative 42) pulmonary cases, sensitivities 93.2 83.3% were observed, respectively with 92.8% specificity, whereas sensitivity 77.9%...

Acinetobacter species are ubiquitous in the environment and important causative agent for nososcomial infection especially immunocompromised patients. Multi drug resistant lwoffii emerging as a pathogen neoanatal sepsis.This study was aimed to evaluate clinical antibiotic profile of lwoffii.This done on blood samples from neonates admitted neonatal intensive care unit during period one year January December 2012, who developed infection. The diagnosis isolates susceptibility testing by both...

Phytotherapy ResearchVolume 30, Issue 6 p. 956-962 Research Article Effect of Subgingivally Delivered 10% Emblica officinalis Gel as an Adjunct to Scaling and Root Planing in the Treatment Chronic Periodontitis – A Randomized Placebo-controlled Clinical Trial Shilpa Grover, Grover Department Periodontics Oral Implantology, Post Graduate Institute Dental Sciences, Rohtak, Haryana, IndiaSearch for more papers by this authorShikha Tewari, Corresponding Author Shikha Tewari India Correspondence...

Aim: Diagnosis of pleural TB poses serious challenges due to paucibacillary nature specimens and there is an urgent need devise a reliable diagnostic test. Methods: We compared GeneXpert Mycobacterium tuberculosis/rifampin assay the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) IS6110 in fluids (n = 78) patients non-TB controls. Results: The sensitivities 89.6 33.3%, specificities 96.7 100%, were observed with M-PCR Xpert assay, respectively. Conclusion: showed superiority over may...

From January 1998 to December 2001, 176 cases of Citrobacter bacteremia occurred which repeat isolation was possible in 48 cases. Of isolates, 79.1% were C. diversus and 20.9% freundii. polymicrobial 46.1% cases, maximum number (54.1%) the age group less than 10 years. Portal entry unknown (42.3%), respiratory tract (20.9%), gastrointestinal (15.3%) urinary 15.3%. freundii isolates relatively more resistant against tested antimicrobial agents, while multiresistant. Sensitivity based on MIC...

In capillary electrophoresis, covering the inner surface with a coating is an efficient way to minimize both electroosmotic flow and sorption of analytes on wall. We modified procedure by Cobb et al. [Anal. Chem. 62, 2478 (1990)] for preparing wall permit large-scale production. Specifically, we use positive pressure fill thionyl chloride later vinylmagnesium bromide solution. This enables production treating 100 m pieces at time. found that no extensive flushing either organic solvents or...

ABSTRACTBackground Diagnosis of pleural tuberculosis (TB) is tedious owing to its close resemblance with malignant effusion and sparse bacterial load in clinical specimens. There an immediate need design a rapid dependable diagnostic test prevent unnecessary morbidity/mortality.Research methods A multi-targeted loop-mediated isothermal amplification (MT-LAMP) was deliberated using mpt64 IS6110 diagnose TB within fluids/biopsies. MT-LAMP products were analyzed by gel-based visual detection...

Antimicrobial resistance causes substantial risks to human health globally, and millions of people die worldwide due multiple drug resistances. Beta-lactam drugs are common for curing infections, these cause serious threat humans. The is acquired by the gram-negative Pseudomonas aeruginosa producing beta-lactamases such as metallo beta-lactamase (MBL), extended-spectrum enzymes (ESBL), AmpC β-lactamases. Hence, this study was intended detect occurrence MBL, ESBL, β-lactamases P. evaluate...

Aim: Diagnosis of urogenital tuberculosis (UGTB) is difficult and there an immediate need to develop a reliable diagnostic test. Methods: A real-time immuno-PCR (RT-I-PCR) was developed identify cocktail MPT-64 + ESAT-6 in both male/female UGTB patients comprising five confirmed cases, 40 clinically suspected cases 37 non-TB controls, from whom mid-stream urine specimens were collected, while endometrial biopsies female obtained on day 1 their menstrual cycle. Results by RT-I-PCR compared...

Cryptosporidiosis, a diarrheal disease caused by the protozoan parasite Cryptosporidium spp. has become recognized as one of most common causes water borne diseases in humans.To compare sensitivity ELISA and Microscopy for detection stool samples Materials Methods: The study was conducted Department Microbiology PT. B.D. Sharma PGIMS Rohtak, between January 2011 to june on 50 samples, which were processed cryptosporidial antigen cysts microscopy (Modified Ziehl Nelsen staining).This...

The pathogen, Rhizoctonia solani has a wide host range infecting crops. In order to find out the of R. causing root rot fenugreek, 13 plant species were selected which are commonly found in fenugreek growing ecosystem. Root caused by become an important constraint growers Rajasthan. cause chick pea, chilli, coriander, cumin, wheat, barley, okra, field tomato, fennel and cabbage but spinach was not infected. Out these plants maximum disease incidence observed paan methi (Trigonella...