A5064798475- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Cellular transport and secretion
- Inhalation and Respiratory Drug Delivery
- Infant Nutrition and Health
- Pancreatic function and diabetes
- Lung Cancer Treatments and Mutations
- Cytokine Signaling Pathways and Interactions
- Lung Cancer Research Studies
- IL-33, ST2, and ILC Pathways
- interferon and immune responses
Oregon Health & Science University
2020-2024
Antigen presentation molecules play key roles in activating T cell immunity. Multiple complementary pathways are known to regulate classical MHC-I at transcriptional, translational, and post-translational levels. Intracellular trafficking mechanisms dictating post-transcriptional regulation of MR1, the MHC Class I-like molecule which restricts MAIT cells, have been an area focus; however, little is about MR1 transcriptional regulation. We demonstrate that, similar MHC-I, interferons...
Abstract MR1-restricted T cells have been implicated in microbial infections, sterile inflammation, wound healing and cancer. Similar to other antigen presentation molecules, evidence supports multiple, complementary MR1 pathways. To investigate ligand exchange pathways for MR1, we used monomers tetramers loaded with 5-(2-oxopropylideneamino)-6-d-ribitylaminouracil (5-OP-RU) deliver the antigen. Using MR1-deficient reconstituted wild-type or molecules that cannot bind 5-OP-RU, show of...
Chronic obstructive pulmonary disease (COPD) is associated with airway inflammation, increased infiltration by CD8+ T lymphocytes, and infection-driven exacerbations. Although cigarette smoke the leading risk factor for COPD, mechanisms driving development of COPD in only a subset smokers are incompletely understood. Lung-resident mucosal-associated invariant (MAIT) cells play role microbial infections inflammatory diseases. The MAIT pathology unknown. Here, we examined cell activation...
Abstract Mucosal-associated invariant T (MAIT) cells are an innate-like cell subset important in the early response to bacterial and viral lung pathogens. MAIT recognize small molecule metabolites presented on Class I-like MR1. As with other I II molecules, MR1 can likely sample ligands intracellular environment through multiple cellular pathways. Rab6, a GTPase that regulates number of endosomal trafficking pathways including retrograde transport trans-Golgi network (TGN), is involved...
Abstract Chronic obstructive pulmonary disease (COPD) is associated with airway inflammation, increased infiltration by CD8 + T lymphocytes, and infection-driven exacerbations. COPD most commonly caused cigarette smoke (CS), however the mechanisms driving development of in some smokers but not others are incompletely understood. Lung-resident mucosal-associated invariant (MAIT) cells play a role both microbial infections inflammatory diseases. MAIT cell frequency reduced peripheral blood...
Abstract MAIT cells, a highly prevalent subset of T cells restricted by the MHC Class I-like molecule MR1, play key role in early response to bacterial infection. Chronic obstructive pulmonary disease (COPD) pathogenesis is associated with airway inflammation, increased infiltration CD8+ lymphocytes, and infection-driven exacerbations. Cigarette smoke (CS), leading cause COPD, leads impaired immune function epithelial (AECs) decreases surface expression classical I molecules. However, impact...
Abstract MAIT cells are a subset of innate-like lung-resident CD8+ T that thought to play role in early immune responses airway infections. These exhibit immediate effector function following recognition bacterial small molecule metabolites presented on the MHC Class I-like molecule, MR1. The impact cigarette smoke and development COPD MR1 cell is unknown. To address this, we obtained primary epithelial from lungs human subjects with (n=6) or healthy controls (n=6). were treated not extract...
Abstract MR1-restricted T cells have been implicated in microbial infections, sterile inflammation, wound healing and cancer. Similar to other antigen presentation molecules, evidence supports multiple, complementary MR1 pathways. To investigate ligand exchange pathways for MR1, we used monomers tetramers loaded with 5-(2-oxopropylideneamino)-6-d-ribitylaminouracil (5-OP-RU) deliver the antigen. Using MR1-deficient reconstituted wild-type or molecules that cannot bind 5-OP-RU, show of...