A5103223709- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Titanium Alloys Microstructure and Properties
- Osteoarthritis Treatment and Mechanisms
- Manufacturing Process and Optimization
- Orthopaedic implants and arthroplasty
- Liver physiology and pathology
- Proteoglycans and glycosaminoglycans research
- Topology Optimization in Engineering
- Advanced Numerical Analysis Techniques
- Silk-based biomaterials and applications
- Collagen: Extraction and Characterization
- Corneal Surgery and Treatments
- Additive Manufacturing and 3D Printing Technologies
Czech Academy of Sciences, Institute of Experimental Medicine
2015-2020
Czech Academy of Sciences
2015-2017
Czech Academy of Sciences, Institute of Biophysics
2015-2017
Charles University
2015-2017
Abstract: In this study, we have developed a combined approach to accelerate the proliferation of mesenchymal stem cells (MSCs) in vitro, using new nanofibrous scaffold made by needleless electrospinning from mixture poly-ε-caprolactone and magnetic particles. The biological characteristics porcine MSCs were investigated while cultured vitro on composite enriched with nanoparticles. Our data indicate that due synergic effect nanofibers particles, cellular adhesion is enhanced osteogenic...
Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique excessive smoothness printed fibers, which does not support cell adhesion. In present study, a scaffold was combined electrospun classic or structured nanofibers to promote Structured were used improve infiltration cells into scaffold. Electrospun layers connected fibers by gluing, thus enabling fabrication unlimited thickness....
Here we introduce a wide and complex study comparing effects of growth factors used alone in combinations on human mesenchymal stem cell (hMSC) proliferation osteogenic differentiation. Certain ways behaviour can be triggered by specific peptides - factors, influencing fate through surface cellular receptors.In our transforming factor β (TGF-β), basic fibroblast (bFGF), hepatocyte (HGF), insulin-like 1 (IGF-1), vascular endothelial (VEGF) were order to induce osteogenesis hMSCs from bone...
Fibrous scaffolds are desired in tissue engineering applications for their ability to mimic extracellular matrix. In this study we compared fibrous prepared from polycaprolactone using three different fabrication methods, electrospinning (ES), electro-blowing and melt-blown combined with ES. Scaffolds differed morphology, fiber diameters pore sizes. Mesenchymal stem cell adhesion, proliferation osteogenic differentiation on was evaluated. The most promising scaffold shown be combination ES...
Platelets are a popular source of native growth factors for tissue engineering applications. The aim the study was to verify use platelet lysate as fetal bovine serum (FBS) replacement skin cell culture. cytokine content characterized using Bio-Plex system. cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds mimic their natural microenvironment. determined, cells, medium containing or in combination with FBS added. results showed that 7% (v/v)...
Abstract Objectives Bioactive peptides derived from receptor binding motifs of native proteins are a potent source bioactive molecules that can induce signalling pathways. These could substitute for osteogenesis promoting supplements. The work presented here compares three kinds collagen III , bone morphogenetic protein 7 ( BMP ‐7) and ‐2 with their potential osteogenic activity on the model porcine mesenchymal stem cells pMSC s). Materials methods s were cultured electrospun...
Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, FTIR analysis confirmed successful blending chitosan with other polymers. The biocompatibility PCL-chit PEO-chit scaffolds was tested;...