A5021583807- Immunotherapy and Immune Responses
- Gut microbiota and health
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Diabetes Treatment and Management
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Viral gastroenteritis research and epidemiology
- Signaling Pathways in Disease
- Regulation of Appetite and Obesity
- Toxoplasma gondii Research Studies
- Microbial infections and disease research
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
Sichuan University
2021-2025
State Key Laboratory of Biotherapy
2021-2025
Chengdu University of Traditional Chinese Medicine
2024-2025
Northeast Agricultural University
2016-2017
Inflammatory bowel diseases (IBDs), such as Crohn's disease (CD) and ulcerative colitis (UC), represent a growing global health concern. Restoring the balance of gut microbiota, crucial factor in intestinal health, offers potential for treating IBD. DP7, novel antimicrobial peptide with potent antibacterial activity, was investigated its anti-inflammatory effects dextran sulfate sodium (DSS)-induced UC mouse model. DP7 significantly ameliorated key parameters, including activity index,...
Abstract The clinical efficacy of personalized cancer vaccines still needs to be improved due their insufficient immune effect. development innovative adjuvants and lymph node‐targeted delivery systems is the key improving vaccines. However, there a lack an adjuvant system that simple in preparation capable mass production integrates node targeted functions. Here, this work reports dendrimer polypeptide (KK2DP7) nanoparticle enhances OVA/neoantigen‐based vaccine. Due its multiple functions...
Abstract Despite the remarkable success of immune checkpoint inhibitors (ICIs), primary resistance to ICIs causes only subsets patients achieve durable responses due complex tumor microenvironment (TME). Oncolytic viruses (OVs) can overcome immunosuppressive TME and promote systemic antitumor immunity in hosts. Engineered OVs armed with would likely have improved effectiveness as a cancer therapy. According diverse cell landscapes among different types tumors, we rationally precisely...
Abstract In situ vaccination is a desirable strategy for cancer immunotherapy due to its convenience and capacity target tumor antigens. Here, an in nanovaccine based on cationic peptide with cholesterol‐modified, DP7‐C, rationally designed, developed that easy preparate. The includes cocktail small interfering RNAs (siRNAs) immunologic adjuvant CpG ODNs, has synergistic effect the treatment. This can induce cell death, promote antigen presentation relieve immune suppression microenvironment...
Abstract Hepatocellular carcinoma (HCC) is one of the most lethal cancers in humans. The inhibition peptidyl‐prolyl cis / trans isomerase (Pin1) gene expression may have great potential treatment HCC. N ‐Acetylgalactosamine (GalNAc) was used to target liver. Cholesterol‐modified antimicrobial peptide DP7 (DP7‐C) acts as a carrier, GalNAc‐siRNA/DP7‐C complex increases uptake GalNAc‐siRNA and escape endosomes hepatocytes. In addition, DP7‐C nanoparticles hydrogel‐assisted GalNAc‐Pin1 siRNA...
Rheumatoid arthritis (RA) is an autoimmune disease with features of synovial inflammation, cartilage erosion, bone destruction, and pain currently lacking a satisfactory treatment strategy. Dihydroartemisinin (DHA), the active metabolite artemisinin, has exhibited outstanding suppressive effects on RA without obvious side effects. However, underlying mechanisms remain unclear, which limits its further clinical application. The purpose this study to reveal pharmacodynamic mechanism DHA...
Mutations at RNA splicing sites or regulatory elements can alter efficiency patterns, affecting functionality and tissue-specific expression. Here, we present a protocol to study the impact of mutations near on precursor mRNA (pre-mRNA) splicing. We describe steps for constructing plasmids by cloning target gene into pEGFP-N1 vector, performing site-directed mutagenesis, transiently transfecting HEK293 cells. then detail procedures conducting mini-gene assays analyze patterns influenced...
The mechanism of colistin-induced neurotoxicity is still unknown. Our recent study (L. Zhang, Y. H. Zhao, W. J. Ding, G. Z. Jiang, Lu, L. Li, Wang, and C. Antimicrob Agents Chemother 59:2189-2197, 2015, http://dx.doi.org/10.1128/AAC.04092-14; T. Zhou, Int J Mol Med 33:1298-1304, 2014, http://dx.doi.org/10.3892/ijmm.2014.1684) indicates that colistin induces autophagy apoptosis in rat adrenal medulla PC-12 cells, there interplay between both cellular events. As an important stress sensor,...
Rituximab (RTX) is a widely used anticancer drug with gastrointestinal side effects, such as nausea, vomiting, and diarrhea. The reason for these effects still poorly understood. Previous studies have reported that the intestinal microbiota associated occurrence of disease therapeutic effect drugs. In this study, we observed mucosal damage, inflammatory cell infiltration increased factor expression in RTX-treated mice. RTX also changed diversity mice, decreased abundance Lactobacillus...
Abstract The development of adjuvants has been an empirical process. Efforts to develop a new design and evaluation system for novel are not only desirable but also necessary. Moreover, composite that contain two or more types synergistically enhance the immune response important adjuvant vaccine design. Innate defense regulator peptides (IDRs) promising clinical immunotherapy because they exhibit multifaceted immunomodulatory capabilities. However, rational discovery IDRs have improved...
Abstract Glioma is a common primary central nervous system malignant tumor in clinical, traditional methods such as surgery and chemoradiotherapy are not effective treatment. Therefore, more treatments need to be found. Oncolytic viruses (OVs) new type of immunotherapy that selectively infects kills cells instead normal cells. OVs can mediate antitumor immune responses through variety mechanisms, have the ability activate responses, transform microenvironment from “cold” “hot,” enhance...
Extensive evidence has demonstrated that glucagon-like peptide-1 receptor agonists (GLP-1RAs) can ameliorate obesity. Our previous studies revealed (Ex-4)2-Fc, a long-acting GLP-1RA we developed, depends on the leptin pathway to treat However, mechanisms linking (Ex-4)2-Fc and resistance remain largely unclear. To address this question, explored mechanism of GLP-1RAs from perspective gut microbiota, as increasing indicates an important link between microbiota This study aimed explore...
Abstract Treatment with immune checkpoint inhibitors (ICIs) has recently achieved unprecedented clinical benefits, becoming a critical treatment for patients cancer. However, set of are resistant to inhibitor therapy, likely due the limited presence or lack tumor‐infiltrating lymphocytes in their tumors. Increasing data indicate that antiangiogenic therapy substantially reduces cancer‐induced immunosuppression and is an effective way enhance efficacy cancer immunotherapies by combination...
Extensive evidence demonstrated that Glucagon-like peptide-1 receptor (GLP-1R) agonists could effectively treat obesity. Our previous studies also proved (Ex-4)2-Fc, a long-acting GLP-1R agonist we developed, improved obesity, and the efficacy depended on leptin pathway. However, mechanisms linked (Ex-4)2-Fc remain largely unclear. We treatment increased Akkermansia muciniphila (Am) its metabolite inosine. Inosine reduced adipocytes secretion in an indirect manner by regulating macrophages...