A5100337528- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cardiovascular Disease and Adiposity
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Phagocytosis and Immune Regulation
- Nanoplatforms for cancer theranostics
- Ultrasound and Hyperthermia Applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Calcium signaling and nucleotide metabolism
- PI3K/AKT/mTOR signaling in cancer
- Spaceflight effects on biology
- Cardiovascular Health and Disease Prevention
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Synthesis and Biological Activity
- Bone Tissue Engineering Materials
- Mosquito-borne diseases and control
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cytokine Signaling Pathways and Interactions
- Pulmonary Hypertension Research and Treatments
- Cardiovascular and exercise physiology
Air Force Medical University
2010-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2024
China Medical University
2022
ShanghaiTech University
2020
Fudan University
2017-2020
National Center for Nanoscience and Technology
2018
Xijing Hospital
2010
Abstract Immunotherapy holds great promise for the treatment of malignant cancer. However, lack sufficient tumor neoantigens and incomplete dendritic cell (DC) maturation compromise efficacy immunotherapy. Here, a modular hydrogel‐based vaccine capable eliciting powerful sustained immune response is developed. Briefly, CCL21a Exo GM‐CSF+Ce6 (tumor cell‐derived exosomes with granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) mRNA encapsulated inside sonosensitizer chlorin e6 (Ce6)...
Cholangiocarcinoma (CCA) and gallbladder carcinoma (GBC) are biliary tract cancers with poor five-year survival high recurrence rates. Both CCA GBC patients suffer from lack of circulating diagnostic biomarkers at the early stage. Extracellular vesicles, especially exosomes, have been emerged as promising sources for due to easy quick accessibility. Hence, identification exosomal provides a novel strategy diagnosis. Here, five four were enrolled small RNA sequencing. Our data showed that...
Regular exercise maintains a healthy metabolic profile, while the underlying mechanisms have not been fully elucidated. Extracellular vesicles serve as an important mediator in intercellular communication. In this study, we aimed to explore whether exercise-induced extracellular (EVs) of skeletal muscle origins contribute exercise-related protective effects on metabolism. We found that twelve weeks swimming training improved glucose tolerance, reduced visceral lipid accumulation, alleviated...
Vascular microenvironment is found to be closely related immunotherapy efficacy. Identification and ultrasound imaging of the unique vascular characteristics, able predict immune microenvironment, important for decision-making. Herein, it proved that high CD93 expression in tumor vessels poor response prostate cancer. For molecular CD93, CD93-targeted microbubbles (MBs) consist a gaseous core MMRN2 (Multimerin-2) containing cell membrane (CM) /lipid hybrid then synthesized. In vitro vivo...
Senescent cells in plaques emerge as a detrimental factor for atherosclerosis (AS), which targeted senolysis might be promising therapeutic strategy. The development of safe and efficient senolytics senescent cell eradication by delivery is greatly needed.
Abstract Unravelling the mechanisms for immunosuppressive tumor microenvironment and developing corresponding therapeutic strategies are of great importance to improve cancer immunotherapy. This study has revealed that there abundant senescent cells accumulated in colon tissue, which contributes greatly microenvironment. Oral delivery Dasatinib Quercetin (D+Q) eliminates with compromised efficiency due poor penetration short half‐life. To efficacy cell clearance, this work developed an...
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease. Whether extracellular vesicles are effective in treating IPF what the optimal administrative route not clear. Our previous studies have shown that immunity matrix regulatory cells (IMRCs) derived from human embryonic stem can safely treat injury mouse models, its mechanism of action related to paracrine effect. In this study, we investigated therapeutic effects IMRC-derived (IMRC-EVs) on...
Rheumatoid arthritis (RA) is an autoimmune disease with features of synovial inflammation, cartilage erosion, bone destruction, and pain currently lacking a satisfactory treatment strategy. Dihydroartemisinin (DHA), the active metabolite artemisinin, has exhibited outstanding suppressive effects on RA without obvious side effects. However, underlying mechanisms remain unclear, which limits its further clinical application. The purpose this study to reveal pharmacodynamic mechanism DHA...
Abstract Exosome is a crucial manner for cancer cell to communication and circulating exosomes sever as promising diagnostic prognostic markers various types of diseases. A predominant type cargo exosome small RNAs, especially miRNAs. Here, we profiled plasma exosomal miRNAs six lung adenocarcinoma patients before after surgery, well healthy individuals normal control. Our profiling revealed 38 upregulated 37 downregulated in the patients. Additionally, found that most were increased samples...
To investigate the effect of air-drying pressure during ceramic primer coating on zirconia/resin bonding and surface characteristics primed zirconia.Two primers (Clearfil Ceramic Primer, CCP, Kuraray Medical Inc. Z-Prime Plus, ZPP, Bisco Inc.) were applied air-abraded zirconia (Katana zirconia, Noritake) dried at 4 different air pressures (0.1-0.4 MPa). The specimens bonded with a resin-based luting agent (SA Luting Cement, Kuraray). Micro-shear bond strengths tested after 3 days water...
Exosome mediated mRNA delivery is a promising strategy for the treatment of multiple diseases. However, low yield exosomes bottleneck clinical translation. In this study, we boosted exosome production via simultaneously reducing expression genes inhibiting biogenesis and supplementing culture medium with red cell membrane components.Among candidate genes, knocking down Rab4 was identified to have highest efficacy in promoting while without any obvious cytotoxicity. Additionally, particles...
Abstract Neuroinflammation mediated by microglia has been identified as vital pathogenesis in Parkinson's disease (PD). This study aimed to investigate the role and potential regulatory mechanism of microRNA‐330 lipopolysaccharide (LPS)‐induced chronic neuroinflammatory model. Primary inflammation model PD animal were established LPS treatment. Bulged sponges containing six microRNA binding sites constructed delivered plasmid or recombinant adeno‐associated virus (rAAV2)/5‐green fluorescent...
Arterial stiffness due to the vessel remodeling is closely linked raised blood pressure, and its physiopathologic mechanism still not fully understood. We here aimed explore whether extracellular vesicle (EV) mediated intercellular communication between endothelium smooth muscle cell contribute under hypertension. revealed that arterial endothelial cells robustly secreted EV, which in turn could be circulated and/or directly taken up by subendothelial (SMC). Under hypertension, EV secretion...
Diabetes vasculopathy is a significant complication of diabetes mellitus (DM), and early identification timely intervention can effectively slow the progression. Accumulating studies have shown that causes vascular complications directly or indirectly through variety mechanisms. Direct imaging endothelial molecular changes not only identifies stage but also sheds light on precise treatment. Targeted ultrasound contrast agent (UCA)-based (UMI) noninvasively detect expression status biomarkers...
Key points Rapid alterations of gravitational stress during high‐performance aircraft push–pull manoeuvres induce dramatic shifts in volume and pressure within the circulation system, which may result loss consciousness due to rapid significant reduction cerebral perfusion. There are still no specific effective countermeasures so far. We found that lower body negative (LBNP), applied prior −Gz released at subsequent transition +Gz, could effectively counteract haemodynamic induced by a...
Objectives In this study, we aimed to investigate the effect of p62 on angiogenesis and microRNA (miRNA) expression profiles in acute myeloid leukemia (AML) exosomes. Methods An Exiqon v19.0 MicroArray was used profile miRNAs exosomes derived from parental p62-knockdown U937 cells. The Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) databases were predict biological functions potential mechanisms differentially expressed AML Endothelial cell tube formation assays using human...
Extracellular vesicle-based diagnosis and molecular phenotyping provide great help for precise treatment of cancer. In article 1800122, Huiping Li, Ling Zhu, Yanlian Yang, co-workers establish an assessment the detection serum extracellular vesicles using microbead-assisted flow cytometry. Molecular is facilitated by immunostaining important biomarkers diagnosis, prognosis, therapeutic evaluation on nanosized that are enriched microsized aldehyde latex beads. This method shows efficacy in...