A5101554116- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Neurotransmitter Receptor Influence on Behavior
- Extracellular vesicles in disease
- Anesthesia and Neurotoxicity Research
- Circular RNAs in diseases
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Intensive Care Unit Cognitive Disorders
- Advanced Fluorescence Microscopy Techniques
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Chemokine receptors and signaling
- Nicotinic Acetylcholine Receptors Study
- Dendrimers and Hyperbranched Polymers
- Nerve injury and regeneration
- Drug Transport and Resistance Mechanisms
- Axon Guidance and Neuronal Signaling
- Protein Interaction Studies and Fluorescence Analysis
- RNA Interference and Gene Delivery
- Medical Imaging Techniques and Applications
- Immunotherapy and Immune Responses
- Nanoparticle-Based Drug Delivery
Harvard University
2014-2024
West China Hospital of Sichuan University
2020-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2024
Sichuan University
2020-2024
West China Medical Center of Sichuan University
2022
Stanford University
2020
National Center for Nanoscience and Technology
2016-2019
University of Chinese Academy of Sciences
2017-2019
Center for Excellence in Education
2016-2019
Center for NanoScience
2016-2019
Information flow in neurons proceeds by integrating inputs dendrites, generating action potentials near the soma, and releasing neurotransmitters from nerve terminals axon. We found that striatum, acetylcholine-releasing induce potential firing distal dopamine axons. Spontaneous activity of cholinergic produced release extended beyond acetylcholine-signaling domains, traveling were readily recorded axons response to activation. In freely moving mice, acetylcholine covaried with movement...
Abstract Background Postoperative cognitive dysfunction (POCD) is a common neurological complication following anesthesia and surgery. Increasing evidence has demonstrated that neuroinflammation caused by systemic inflammatory responses during the perioperative period key factor in occurrence of POCD. In addition, SMAD family member 7 (Smad7) been confirmed to play vital roles pathogenesis treatment diseases, such as bowel disease. However, whether Smad7 participates regulatory process...
Stimulation of the prefrontal cortex (PFC) has been shown to have an excitatory influence on dopamine (DA) neurons. We report here that, under nonstimulated conditions, activity DA neurons in ventral tegmental area (VTA) also covaries, a subsecond timescale, with PFC cells. Thus, 67% VTA recorded chloral hydrate-anesthetized rats, firing cell displayed slow oscillation (SO) that was highly coherent The SO suppressed by transections immediately caudal or intra-PFC infusion tetrodotoxin,...
α-Mangostin, a component of Garcinia mangostana Linn, is xanthone derivative shown to have antioxidant and anticarcinogen properties. In this study, we first report the antimetastatic effect α-mangostin in human prostate carcinoma cell line PC-3. The results show that exhibited an inhibitory on abilities adhesion, migration, invasion by cell-matrix adhesion assay, wound healing Boyden chamber assay. cancer metastasis process, matrix degrading proteinases are required. Results from zymography...
In a presynaptic nerve terminal, synaptic vesicle exocytosis is restricted to specialized sites called active zones. At these sites, neurotransmitter release determined by the number of releasable vesicles and their probability release. Proteins at zone set parameters controlling Ca 2+ signal, through docking priming vesicles. Vertebrate ELKS proteins are enriched zones, but functions not well understood. produced two genes in vertebrates, each gene contributes ∼50% total brain ELKS. We...
Significance The presynaptic active zone is important for precise and fast information transfer at central nervous synapses. Invertebrate studies have indicated that Liprin-α proteins are assembly function, but localization function of vertebrate remained enigmatic, pre- or postsynaptic roles been proposed. Here we used stimulated emission depletion microscopy to directly show Liprin-α3, not Liprin-α2, prominently localizes the zone. We then generated Liprin-α3–KO mice found structure...
In a presynaptic nerve terminal, synaptic strength is determined by the pool of readily releasable vesicles (RRP) and probability release (P) each RRP vesicle. These parameters are controlled at active zone vary across synapses, but how such synapse specific control achieved not understood. ELKS proteins enriched vertebrate zones enhance P inhibitory hippocampal functions excitatory synapses known. Studying conditional knockout mice for ELKS, we find that enhances without affecting P....
Dopamine powerfully controls neural circuits through neuromodulation. In the vertebrate striatum, dopamine adjusts cellular functions to regulate behaviors across broad time scales, but how secretory system is built support fast and slow neuromodulation not known. Here, we set out identify Ca2+-triggering mechanisms for release. We find that synchronous secretion abolished in acute brain slices of conditional knockout mice which Synaptotagmin-1 removed from neurons. This indicates Ca2+...
Chronic pain is often associated with cognitive decline, which could influence the quality of patient's life. Recent studies have suggested that Toll-like receptor 3 (TLR3) crucial for memory and learning. Nonetheless, contribution TLR3 to pathogenesis decline after chronic remains unclear. The level in hippocampal neurons increased constriction injury (CCI) group than sham this study. Importantly, compared wild-type (WT) mice, knockout (KO) mice TLR3-specific neuronal knockdown both...
Glioblastoma is one of the most common primary tumor types central nervous system (CNS) with high malignance and lethality.Although many treatment options are currently available, therapy brain cancers remains challenging because blood-brain-barrier (BBB) which prevents chemotherapeutics into CNS.In this work, a poly(amidoamine) dendrimer-based carrier was fabricated modified angiopep-2 (Ang2) peptide that has been demonstrated to bind low density lipoprotein receptor-relative protein-1...
Although nanomedicine has demonstrated great potential for combating drug resistance, its suboptimal recognition of malignant cells and limited transport across multiple biological obstacles seriously impede the efficacious accumulation drugs in tumor lesions, which strikingly limits application clinical therapy drug-resistant triple-negative breast cancer (TNBC). Hence, a surface-variable delivery vehicle based on modification liposomes with multifunctional peptide (named EMC) was...
Abstract Blood‐based detection and molecular phenotyping are highly desired for the early diagnosis dynamic monitoring of cancer. Extracellular vesicles (EVs) carry information from cells origin biomarkers However, analysis EVs has been challenging due to their nanoscaled size. Here, an assessment serum based on microbead‐assisted flow cytometry is established. The clinical utility this method validated in human epidermal growth factor receptor 2 (HER2) breast Good correlation between status...
Background: Chronic neuropathic pain is commonly associated with memory loss, which increases the risk of dementia, lowers life quality and spending. On other hand, molecular processes are unknown, effective therapies have yet to be discovered. Long non-coding RNAs (lncRNAs) emerging potential therapeutic targets for chronic pain, but their role in pain-induced impairment unknown. Methods: We established a CCI-induced rat model. To investigate validate gene expression alterations hippocampus...
Background: Postoperative cognitive dysfunction (POCD) is one of the severe complications inducing low life quality and high mortality after surgery, especially in elderly patients. However, underlying molecular mechanism POCD remains largely unknown ideal biomarker for clinical diagnosis prognosis lacking. Circular RNAs (circRNAs), as a unique class noncoding (ncRNAs), was characterized by its stably conservativeness serving novel biomarkers various disease. Nevertheless, role circRNAs...