A5102868762- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Machine Learning in Bioinformatics
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Osteoarthritis Treatment and Mechanisms
- Zebrafish Biomedical Research Applications
- Genomics and Chromatin Dynamics
University of British Columbia
2024
École Polytechnique Fédérale de Lausanne
2024
Hospital for Sick Children
2010
University of Toronto
2010
Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting computation function. In thalamus, anterior thalamic nuclei (ATN) are involved in a wide variety functions, yet ATN remains unmapped at single-cell resolution. Combining RNA sequencing, transcriptomics, multiplexed fluorescent situ hybridization, we identify three discrete excitatory clusters that correspond to known uncover marker genes, molecular pathways, putative functions these...
We introduce Vespucci, a machine-learning method to identify perturbation-responsive regions, genes and gene programs within comparative spatial transcriptomics atlases. validate Vespucci on simulated published datasets show that it outperforms 19 computational methods for transcriptomics. apply expose the organization of activated by therapies guide repair injured spinal cord.
Classifying proteins into subgroups with similar molecular function on the basis of sequence is an important step in deriving reliable functional annotations computationally. So far, however, available classification procedures have been evaluated against protein that are defined by experts using mainly qualitative descriptions function. Recently, vitro DNA-binding preferences to all possible 8-nt DNA sequences measured for 178 mouse homeodomains protein-binding microarrays, offering...
Abstract Recent discoveries that splicing factors such SF3B1, U2AF1, SRSF2 are frequently mutated in multiple hematological malignancies including chronic lymphocytic leukaemia and myelodysplastic syndromes have generated interest therapeutic approaches to target the splicesome dependency cancer cells bearing mutations factors. Previously, several structurally unrelated natural compounds pladienolide, herboxidiene, FR901464 been shown exert potent anti-proliferative effects grown vitro....
Abstract Dysregulation of RNA splicing can cause various forms cancer and neuromuscular disorders. Thus, developing compounds with splicing-modulating activity represents a promising therapeutic approach for these diseases. Natural products such as pladienolide, herboxidiene, spliceostatin have been identified potent modulators that bind SF3B1, member the SF3b subcomplex assembles into U2 snRNP. Using integrated chemogenomic, structural biochemical approaches, we show PHF5A, another core...