A5093048955- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Animal Genetics and Reproduction
University of Washington
2023-2024
Abstract Robust control over gene translation at arbitrary mRNA targets is an outstanding challenge in microbial synthetic biology. The development of tools that can regulate will greatly expand our ability to precisely genes across the genome. In Escherichia coli, most are contained multi-gene operons, which subject polar effects where targeting one for repression leads silencing other same operon. These pose a independently regulating individual operons. Here, we use CRISPR-dCas13 address...
Engineering metabolism to efficiently produce chemicals from multi-step pathways requires optimizing multi-gene expression programs achieve enzyme balance. CRISPR-Cas transcriptional control systems are emerging as important tools for programming expression, but poor predictability of guide RNA folding can disrupt control. Here, we correlate efficacy modified RNAs (scRNAs) CRISPR activation (CRISPRa) in E. coli with a computational kinetic parameter describing scRNA rate into the active...
ABSTRACT Engineering bacterial metabolism to efficiently produce chemicals and materials from multi-step pathways requires optimizing multi-gene expression programs achieve enzyme balance. CRISPR-Cas transcriptional control systems are emerging as important metabolic engineering tools for programming regulation. However, poor predictability of guide RNA folding can disrupt balance through unreliable control. We devised a set computational parameters that describe folding, we expect them be...
Abstract Robust control over gene translation at arbitrary mRNA targets is an outstanding challenge in microbial synthetic biology. The development of tools that can regulate will greatly expand our ability to precisely genes across the genome. In E. coli , most are contained multi-gene operons, which subject polar effects where targeting one for repression leads silencing both genes. These pose a independently regulating individual operons. Here, we use CRISPR-dCas13 address this challenge....