A5037279340- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Crystallization and Solubility Studies
- Single-cell and spatial transcriptomics
- Enzyme Structure and Function
- Acute Myeloid Leukemia Research
- Synthesis and biological activity
- Biopolymer Synthesis and Applications
- Spectroscopy and Chemometric Analyses
- Coordination Chemistry and Organometallics
- Chemical Reaction Mechanisms
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Reactions
Harvard University
2023
Illinois Institute of Technology
2004
Purdue University West Lafayette
2004
In this study, the application of Raman spectroscopy to quantitative in-line monitoring crystallization from solution an enantiotropic polymorphic system, flufenamic acid, is explored. Separate feasibility studies were performed evaluate utility quantitatively monitor changes in concentration and measure composition. The acid was then monitored as a function temperature time using immersion probe coupled spectrometer. spectral data contained information about both phase crystallized solids....
DNA methyltransferase 3A (DNMT3A) is a de novo cytosine responsible for establishing proper methylation during mammalian development. Loss-of-function (LOF) mutations to DNMT3A, including the hotspot mutation R882H, frequently occur in developmental growth disorders and hematological diseases, clonal hematopoiesis acute myeloid leukemia. Accordingly, identifying mechanisms that activate DNMT3A of both fundamental therapeutic interest. Here, we applied base editor mutational scanning strategy...
The highly exothermic nature of Reformatsky reagent formation and the reported unpredictability induction time for its pose challenging problems scaling up reactions. A zinc-activation procedure using DIBAL-H was developed investigated reaction calorimetry along with subsequent parts process. This shown to have important advantages scale-up relative previous zinc activation methods, including an immediate start addition-controlled reaction. Calorimetric analysis especially useful in...
LAB687 is an inhibitor of microsomal triglyceride transfer protein (MTP) designed to lower triglycerides and LDL cholesterol. The discovery its polymorphic forms closely intertwines with the synthesis development molecule. At early stage, was known crystallize in two modifications, Forms A B. Knowledge molecule's nature prompted extensive screening using drug substance produced by earlier routes. These studies revealed existence a third polymorph, Form C. Subsequently, C selected for further...
Abstract DNA methyltransferase 3A (DNMT3A) is a de novo cytosine responsible for establishing proper methylation during mammalian development. Loss-of-function (LOF) mutations to DNMT3A, including the hotspot mutation R882H, frequently occur in developmental growth disorders and hematological diseases, clonal hematopoiesis (CH) acute myeloid leukemia (AML). Accordingly, identifying mechanisms that activate DNMT3A of both fundamental therapeutic interest. Here, we applied base editor...