A5040378366- Laser-Plasma Interactions and Diagnostics
- Laser-induced spectroscopy and plasma
- Nuclear Physics and Applications
- Epigenetics and DNA Methylation
- Atomic and Molecular Physics
- Laser Design and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- CRISPR and Genetic Engineering
- High-pressure geophysics and materials
- Cancer Genomics and Diagnostics
- Plasma Diagnostics and Applications
- Genetics and Neurodevelopmental Disorders
- Sensor Technology and Measurement Systems
- Geology and Paleoclimatology Research
- Single-cell and spatial transcriptomics
- HIV Research and Treatment
- Organometallic Compounds Synthesis and Characterization
- Rocket and propulsion systems research
- Laser-Matter Interactions and Applications
- Acute Myeloid Leukemia Research
- Paleontology and Stratigraphy of Fossils
- Genomics and Chromatin Dynamics
- Energetic Materials and Combustion
- Spectroscopy and Laser Applications
- Ion-surface interactions and analysis
Harvard University
2022-2023
Broad Institute
2022-2023
Energetics (United States)
2017-2021
University of Rochester
2015-2021
Lawrence Livermore National Laboratory
2017-2018
General Atomics (United States)
2018
Los Alamos National Laboratory
2018
Indiana University Bloomington
2018
Massachusetts Institute of Technology
2018
Atomic Weapons Establishment
2018
Allostery enables dynamic control of protein function. A paradigmatic example is the tightly orchestrated process DNA methylation maintenance. Despite fundamental importance allosteric sites, their identification remains highly challenging. Here, we perform CRISPR scanning on essential maintenance machinery—DNMT1 and its partner UHRF1—with activity-based inhibitor decitabine to uncover mechanisms regulating DNMT1. In contrast non-covalent DNMT1 inhibition, selection implicates numerous...
High-intensity laser facilities, such as the National Ignition Facility (NIF), enable experimental investigation of plasmas under extreme, high-energy-density conditions. Motivated by validating models for collisional heat-transfer processes in plasmas, we have developed an exploding pusher platform use at NIF polar-direct-drive configuration. The baseline design employs a 3 mm-diameter capsule, 18 μm-thick CH ablator, and Ar-doped D2 gas to achieve several keV electron-ion temperature...
Abstract Polar direct drive neutron source experiments were performed at the National Ignition Facility showing substantial improvement in total yield and efficiency of conversion laser energy to fusion output. Plastic capsules 3–4 mm diameter filled with 1.5 mg/cc deuterium–tritium (DT) fuel imploded beam pointing defocus designed compensate for polar asymmetry introduced by facility entrance angles. Radiation-hydrodynamics simulations employed optimize multi-dimensional target parameter...
A monoenergetic, isotropic proton source suitable for radiography applications has been demonstrated at the National Ignition Facility (NIF). deuterium and helium-3 gas-filled glass capsule was imploded with 39 kJ of laser energy from 24 NIF's 192 beams. Spectral, spatial, temporal measurements 15-MeV product (3)He(d,p)(4)He nuclear reaction reveal a bright (10(10) protons/sphere), monoenergetic (ΔE/E = 4%) spectrum compact size (80 μm) emission (∼13% fluence variation <0.4% mean variation)....
Direct drive implosions of plastic capsules have been performed at the National Ignition Facility to provide a broad-spectrum (500–2000 eV) X-ray continuum source for transmission spectroscopy. The was developed high-temperature plasma opacity experimental platform. Initial experiments using 2.0 mm diameter polyalpha-methyl styrene with ∼20 μm thickness performed. yields up ∼1 kJ/sr measured Dante multichannel diode array. backlighter size be ∼100 FWHM, ∼350 ps pulse duration during peak...
Extended X-ray absorption Fine Structure (EXAFS) measurements require a bright, spectrally smooth, and broad-band x-ray source. In laser facility, such an source can be generated by laser-driven capsule implosion. order to optimize the emission, different types irradiations have been tested at National Ignition Facility (NIF). A crystal spectrometer is used disperse x-rays high efficiency image plate detectors are measure spectra in transmission geometry. EXAFS K-edge of iron ambient...
Extended X-ray absorption fine structure (EXAFS) spectroscopy is a powerful tool for in situ characterization of matter the high energy density regime. An EXAFS platform currently being developed on National Ignition Facility. Development suitable backlighter involves minimizing temporal duration and source size while maximizing spectral smoothness brightness. One approach imploding spherical shell, which generates high-flux flash at stagnation. We present results from series experiments...
DNA methyltransferase 3A (DNMT3A) is a de novo cytosine responsible for establishing proper methylation during mammalian development. Loss-of-function (LOF) mutations to DNMT3A, including the hotspot mutation R882H, frequently occur in developmental growth disorders and hematological diseases, clonal hematopoiesis acute myeloid leukemia. Accordingly, identifying mechanisms that activate DNMT3A of both fundamental therapeutic interest. Here, we applied base editor mutational scanning strategy...
Polar-direct-drive exploding pushers are used as a high-yield, low-areal-density fusion product source at the National Ignition Facility with applications including diagnostic calibration, nuclear security, backlighting, electron-ion equilibration, and nucleosynthesis-relevant experiments. In this paper, two different paths to improving performance of platform explored: (i) optimizing laser drive, (ii) target. While present study is specifically geared towards nucleosynthesis experiments,...
Abstract DNA methylation is critical for regulating gene expression, necessitating its accurate placement by enzymes such as the methyltransferase DNMT3A. Dysregulation of this process known to cause aberrant development and oncogenesis, yet how DNMT3A regulated holistically three domains remains challenging study. Here we integrate base editing with a reporter perform in situ mutational scanning cells. We identify mutations throughout protein that perturb function, including ones at an...
Abstract DNA methyltransferase 3A (DNMT3A) is a de novo cytosine responsible for establishing proper methylation during mammalian development. Loss-of-function (LOF) mutations to DNMT3A, including the hotspot mutation R882H, frequently occur in developmental growth disorders and hematological diseases, clonal hematopoiesis (CH) acute myeloid leukemia (AML). Accordingly, identifying mechanisms that activate DNMT3A of both fundamental therapeutic interest. Here, we applied base editor...
Proton radiography is a well-established technique for measuring electromagnetic fields in high-energy-density plasmas. Fusion reactions producing monoenergetic particles, such as D3He, are commonly used source, produced by capsule implosion. Using smaller capsules applications advantageous the source size decreases, but on National Ignition Facility (NIF), this can introduce complications from increasing blow-by light, since phase plate focal spot much larger than capsules. We report...
Abstract Allostery enables dynamic control of protein function. A paradigmatic example is the tightly orchestrated process DNA methylation maintenance. Despite their fundamental importance, systematic identification allosteric sites remains highly challenging. Here we perform CRISPR scanning on essential maintenance machinery—DNMT1 and its partner UHRF1—with activity-based inhibitor decitabine to uncover mechanisms regulating DNMT1. Through computational analyses, identify putative...