A5066839248- Glioma Diagnosis and Treatment
- Polyamine Metabolism and Applications
- Single-cell and spatial transcriptomics
- Metabolomics and Mass Spectrometry Studies
- Immune Cell Function and Interaction
- Advanced Proteomics Techniques and Applications
- Epigenetics and DNA Methylation
- Mass Spectrometry Techniques and Applications
- Optical Polarization and Ellipsometry
- MRI in cancer diagnosis
- Mathematical Biology Tumor Growth
- Immune cells in cancer
Walter and Eliza Hall Institute of Medical Research
2023-2024
High-grade gliomas (HGGs) are aggressive primary brain cancers with poor response to standard regimens, driven by immense heterogeneity. In isocitrate dehydrogenase (IDH) wild-type HGG (glioblastoma, GBM), increased intratumoral heterogeneity is associated more disease.Spatial technologies can dissect complex within the tumor ecosystem preserving cellular organization in situ. We employed GeoMx digital spatial profiling, CosMx molecular imaging, Xenium situ mapping and Visium gene expression...
Matrix-assisted laser desorption/ionization mass spectrometry imaging allows for the study of metabolic activity in tumor microenvironment brain cancers. The detectable metabolites within these tumors are contingent upon choice matrix, deposition technique, and polarity setting. In this study, we compared performance three different matrices, two techniques, use positive negative cancer types across species. Optimal combinations were confirmed by a comparative analysis lipid small-molecule...
The glioblastoma (GBM) microenvironment is enriched in immunosuppressive factors that potently interfere with the function of cytotoxic T lymphocytes. Cancer cells can directly impact immune system, but mechanisms driving these interactions are not completely clear. Here we demonstrate polyamine metabolite spermidine (SPD) elevated GBM tumor microenvironment. Exogenous administration SPD drives aggressiveness an immune-dependent manner pre-clinical mouse models via reduction CD8+ cell...
The glioblastoma microenvironment is enriched in immunosuppressive factors that potently interfere with the function of cytotoxic T lymphocytes. Cancer cells can directly impact immune system, but mechanisms driving these interactions are not completely clear. Here we demonstrate polyamine metabolite spermidine elevated tumor microenvironment. Exogenous administration drives aggressiveness an immune-dependent manner pre-clinical mouse models via reduction CD8+ cell frequency and phenotype....
ABSTRACT High-grade gliomas are aggressive primary brain cancers with poor response to standard regimens, driven by immense heterogeneity. In isocitrate dehydrogenase ( IDH ) wild-type high-grade glioma (glioblastoma, GBM), increased intra-tumoral heterogeneity is associated more disease. Recently, spatial technologies have emerged dissect this complex within the tumor ecosystem preserving cellular organization in situ . Here, we construct a high-resolution molecular landscape of GBM and...
Abstract Astrocytoma is a diffuse tumor that infiltrates the brain. Despite post-operative therapy including radiation and chemotherapy, 52% of patients’ tumors recur within five-years surgery. These are characterized by mutations in IDH1 or IDH2 gene confer metabolic alterations, most notably production onco-metabolite 2-hydroxyglutarate. To reveal alterations their upstream regulation across microenvironment, we applied spatial metabolomics transcriptomics to sequential sections from...
Abstract BACKGROUND Astrocytoma is a diffuse and heterogeneous glioma that infiltrates the brain. Despite post-operative therapy including radiation chemotherapy, 52% of patients’ tumours recur within five-years surgery. These are characterised by mutations in IDH1 or IDH2 gene confer metabolic alterations, most notably production onco-metabolite 2-hydroxyglutarate. To reveal alterations their upstream regulation across tumour microenvironment, we applied spatial metabolomics transcriptomics...
Abstract Matrix-assisted laser desorption/ionization mass spectrometry imaging allows the study of metabolic activity in tumor microenvironment brain cancers. The detectable metabolites within these tumors are contingent upon choice matrix, deposition technique, and polarity setting. In this study, we compared performance three different matrices, two techniques, use positive negative cancer types across species. Optimal combinations were confirmed by comparative analysis lipid small...