Cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) enhances humoral immune responses against norovirus (GII.4) virus-like particles

• DOI: 10.3760/cma.j.issn.0254-5101.2017.10.007
• Publication Date: 2017-10-31
• Authors: Jing Chen; Mei‐Ying Liu; Chongfa Tang; Yu Liu; Youxiu Zhong; Fang Tang; Zhijing Ma; Junwei Hou; Bo Wei
• Keywords: Virus-like particle; Humoral immunity

Objective To evaluate the immunopotentiating effect of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) as an adjuvant on norovirus (GⅡ.4) virus like particles (VLPs) in development vaccine.

Methods BALB/c mice were intramuscularly immunized with VLPs composed capsid protein VP1 combination cGAMP or Al(OH)3. Norovirus VLPs-specific antibodies serum detected by ELISA. A synthetic histo-blood group antigen (HBGA)-VLPs blocking assay was used to analyze neutralizing against samples.

Results Immunization presence induced a strong humoral immune response BALB/c mice. Levels specific IgG using significantly higher than those Al(OH)3 when immunization same dosage VLPs. The antibody level 1 μg equivalent that elicited 10 combined adjuvant. Results HBGA-VLPs showed rate cGAMP+ Al(OH)3+ No significant difference observed between (1 μg) and (10 groups.

Conclusion cGAMP enhanced compared It might be novel replace traditional aluminum vaccine.

Key words: cGAMP; Norovirus; Virus (VLPs); Aluminum