A5101533505- Force Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Surface and Thin Film Phenomena
- Antimicrobial agents and applications
- Oral microbiology and periodontitis research
- Mechanical and Optical Resonators
- Alzheimer's disease research and treatments
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- 3D Printing in Biomedical Research
- Protein Structure and Dynamics
- Polydiacetylene-based materials and applications
- Bacterial Genetics and Biotechnology
- Molecular Junctions and Nanostructures
- Marine Biology and Environmental Chemistry
- Listeria monocytogenes in Food Safety
- Nanofabrication and Lithography Techniques
- Antimicrobial Peptides and Activities
- Curcumin's Biomedical Applications
- Bone and Dental Protein Studies
- Magnetic Properties and Synthesis of Ferrites
Danish National Metrology Institute
2018-2023
Danmarks Nationalbank
2023
Aarhus University
2012-2021
DHI
2018-2021
Novo Nordisk (Denmark)
2021
Center for NanoScience
2016
Tsinghua University
2005-2013
Jilin University
2005-2007
Technical Institute of Physics and Chemistry
2005
We have developed an unconventional method for the layer-by-layer (LbL) assembly of graphene multilayer films. Unconventional LbL was achieved by following two-step process. Graphene sheets were modified pyrene-grafted poly(acrylic acid) (PAA) in aqueous solution, and then used alternating deposition with poly(ethyleneimine) (PEI). The graphene-multilayer-film-modified electrode shows enhanced electron transfer redox reactions Fe(CN)63− excellent electrocatalytic activity H2O2. On basis this...
The success of Pseudomonas species as opportunistic pathogens derives in great part from their ability to form stable biofilms that offer protection against chemical and mechanical attack. extracellular matrix contains numerous biomolecules, it has recently been discovered one the components includes β-sheet rich amyloid fibrils (functional amyloid) produced by fap operon. However, role functional within biofilm not yet investigated detail. Here we investigate how fap-based affects...
Epigallocatechin-3-gallate (EGCG) is the major polyphenol in green tea. It has antimicrobial properties and disrupts ordered structure of amyloid fibrils involved human disease. The effect EGCG against opportunistic pathogen Pseudomonas aeruginosa been shown to involve disruption quorum sensing (QS). Functional P. (Fap) are able bind retain quorum-sensing molecules, suggesting that interferes with QS through structural remodeling fibrils. Here we show inhibits ability Fap form fibrils;...
Bringing the study of bacterial adhesion down to a single-cell level is critical for understanding molecular mechanisms involved in initial attachment. We have developed simple and versatile method making probes single cells by atomic force microscopy (AFM). A probe was made picking up cell from glass surface using tipless AFM cantilever coated with commercial adhesive Cell-Tak. The applied four different strains, measured on three surfaces (fresh glass, hydrophilic mica). Attachment stable...
Copper ferrite (CuFe2O4) magnetic hollow fibers were prepared by applying an organic sol-thermal decomposition method, and silver nanoparticles subsequently loaded on the calcination. The Ag–CuFe2O4 exhibited excellent antibacterial efficacy against four different bacteria (E. coli, S. typhi, aureus V. parahaemolyticus) with consistent results. Typical ferromagnetism behavior from enables their feasible recyclability.
We have combined hydrogen-bonding complexation in solution and layer-by-layer assembly for the controlled loading of a water-insoluble small organic molecule, bis-triazine (DTA), an azobenzene derivative containing multiple hydrogen bond donors acceptors, into multilayer films poly(acrylic acid) diazo-resin. UV-visible spectroscopy indicates that DTA has been loaded films, with amount increasing linearly number layers. The can be well tuned either by changing concentration or solvent...
Propionibacteria are part of the human microbiota. Many studies have addressed predominant colonizer sebaceous follicles skin, Propionibacterium acnes, and investigated its association with skin disorder acne vulgaris, lately prostate cancer. Much less is known about two other propionibacterial species frequently found on tissue sites, granulosum avidum. Here we analyzed three genomes P. avidum, respectively, compared them to acnes; further highlight differences among cutaneous proteomic...
Correlative scanning probe microscopy of chemical identity, surface potential, and mechanical properties provide insight into the structure-function relationships nanomaterials. However, simultaneous measurement with comparable high resolution is a challenge. We seamlessly integrated nanoscale photothermal infrared imaging Coulomb force detection to form peak infrared-Kelvin (PFIR-KPFM), which enables nanomapping absorption, approximately 10 nm spatial in single-pass scan. MAPbBr3 perovskite...
We describe a method to fabricate single-charged functional species of sodium 9-anthracenepropionate (SANP) into poly(diallyldimethylammonium chloride) (PDDA)/poly(4-styrenesulfonate) (PSS) multilayer film by precursor assembly in bulk and then layer-by-layer (LbL) deposition at interface. By formation PDDA−SANP complex aqueous solution alternation with PSS, SANP was successfully introduced PDDA/PSS assemblies. Our detailed experiment revealed that the release rate from related concentration...
Many bacteria in biofilm surround themselves by an extracellular matrix composed mainly of polysaccharide (EP), proteins such as amyloid-like fibers (ALF) and nucleic acids. While the importance EP attachment acceleration a number different bacterial species is well established, contribution ALF to multispecies remains unknown. The study presented here aimed investigate role TasA, precursor for ALF, cell-cell interactions dual-species biofilms Bacillus subtilis Streptococcus mutans....
Abstract Self-assembly and fibril formation play important roles in protein behaviour. Amyloid is well-studied due to its role neurodegenerative diseases characterized by refolding of the into predominantly β-sheet form. However, much less known about assembly proteins other types supramolecular structures. Using cryo-electron microscopy at a resolution 1.97 Å, we show that triple-mutant anti-microbial peptide plectasin, PPI42, assembles helical non-amyloid fibrils. The vitro activity was...
Pathogenic bacteria can spread between individuals or food items via the surfaces they share. Limiting survival of pathogens on surfaces, therefore, presents an opportunity to limit at least one route how spread. In this study, we propose that a simple coating with essential oil isoeugenol be used circumvent problem bacterial transfer surfaces.Two commonly materials, stainless steel and polyethylene, were coated by physical adsorption, coatings characterized Raman spectroscopy, atomic force...
Atomic force microscopy (AFM) has emerged as a popular tool for the mechanical mapping of soft nanomaterials due to its high spatial and resolution. Its applications in rigid nanomaterials, however, have been underexplored. In this work, we studied elasticity common materials by AFM, with focus on factors that affect accuracy measurements. We demonstrated advantages speed noise level using frequency compared classical volume mapping. loading dependency, observed consistent pattern all...
Fouling is a major concern for solid/liquid interfaces of materials used in different applications. One approach fouling control the use hydrophilic polymer coatings made from poly-anions and poly-cations using layer-by-layer (LBL) method. The authors hypothesized that poly-anionic properties poly-phosphate backbone DNA would provide anti-biofouling anti-scaling properties. To this end, poly(ethyleneimine)/DNA LBL against microbial inorganic were developed, characterized evaluated. reduced...
Introduction.Staphylococcus epidermidis is predominant in implant-associated infections due to its capability form biofilms. It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the matrix-binding protein (Embp).Hypothesis/Gap Statement. We hypothesize that dichotomic regulation of S. adhesins linked whether it inside a host or not, and vitro investigations laboratory media may not...
Flake thickness is one of the defining properties graphene-related 2D materials (GR2Ms), and therefore requires reliable, accurate, reproducible measurements with well-understood uncertainties. This needed regardless production method or manufacturer because it important for all GR2M products to be globally comparable. An international interlaboratory comparison on graphene oxide flakes using atomic force microscopy has been completed in technical working area 41 versailles project advanced...
We introduced single-charged molecules into layer-by-layer film of polymers by mixing polyelectrolyte and with an appropriate ratio to form polymer complex then deposition in alternation counter-polyelectrolyte. By immersing the aqueous solution electrolytes, could be released from film. An interesting finding is releasing gave multilayer permselectivity. For example, cations would trapped anions repelled if positive-charged were used. Multilayer films able trap either or fabricated,...
Abstract The 2019 update to the Mise en Pratique for metre adopted lattice parameter of silicon as a secondary realisation dimensional nanometrology. One route this is use amphitheatre like monoatomic steps silicon. In response, in paper we present new algorithms one- and two-dimensional analysis atomic force microscope images these large area terraces on surface These can be used determine spacing between identify errors AFM scanning systems. Since vertical separation same order magnitude...
Oligonucleotides (OND) represent a promising therapeutic approach. However, their instability and low intestinal permeability hamper oral bioavailability. Well-established for delivery, self-emulsifying drug delivery systems (SEDDS) can overcome the weakness of other such as long-term nanoparticles or complicated formulation processes. Therefore, present study aims to prepare SEDDS nonspecific fluorescently labeled OND across Caco-2 monolayer. The hydrophobic ion pairing an cationic lipid...
Self-assembled structures from aromatic dipeptides have attracted a lot of attention. It is highly desirable to produce dipeptide assemblies which undergo structural transitions in response external stimuli. In this paper, solid nanospheres were successful produced the self-assembly chemically modified diphenylalanine hexafluoroisopropanol (HFIP), polar solvent. Interestingly, after treatment with water, transformed into nanofibers. The intermediate transition state embedded along nanofibers...
The bovine milk protein osteopontin (OPN) may be an efficient means to prevent bacterial adhesion dental tissues and control biofilm formation. This study sought determine what extent OPN impacts forces surface attachment of different strains involved in caries or medical device–related infections. It further investigated if OPN's effect on is caused by blocking the accessibility glycoconjugates surfaces. Bacterial was determined a shear-controlled flow cell system presence concentrations...
Abstract Correlative scanning probe microscopy of chemical identity, surface potential, and mechanical properties provide insight into the structure–function relationships nanomaterials. However, simultaneous measurement with comparable high resolution is a challenge. We seamlessly integrated nanoscale photothermal infrared imaging Coulomb force detection to form peak infrared–Kelvin (PFIR‐KPFM), which enables nanomapping absorption, approximately 10 nm spatial in single‐pass scan. MAPbBr 3...