A5049708168- Genomics, phytochemicals, and oxidative stress
- Bone and Joint Diseases
- Circadian rhythm and melatonin
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Neuroblastoma Research and Treatments
- Immune cells in cancer
- Reproductive System and Pregnancy
- Glioma Diagnosis and Treatment
- Corneal Surgery and Treatments
- Cytokine Signaling Pathways and Interactions
- Advanced Glycation End Products research
- Redox biology and oxidative stress
Second Affiliated Hospital of Nanjing Medical University
2019-2022
Soochow University
2016
Excessive UV radiation and reactive oxygen species (ROS) cause retinal pigment epithelium (RPE) cell injuries. Nrf2 regulates transcriptional activation of many anti-oxidant genes. Here, we tested the potential role 3H-1,2-dithiole-3-thione (D3T) against or ROS damages in cultured RPE cells (both primary ARPE-19 line). We showed that D3T significantly inhibited UV-/H2O2-induced death apoptosis. UV-stimulated production was dramatically by pretreatment. induced phosphorylation cells, causing...
Neuroligin-3 (NLGN3) is necessary and sufficient to promote glioma cell growth.The recruitment of Gαi1/3 the ligand-activated receptor tyrosine kinases (RTKs) essential for mediating oncogenic signaling.Methods: Various genetic strategies were utilized examine requirement in NLGN3-driven growth.Results: NLGN3-induced Akt-mTORC1 Erk activation was inhibited by decreasing expression.In contrast ectopic overexpression enhanced signaling.In cells, growth, proliferation migration attenuated...
IL-4 induces Akt activation in macrophages, required for full M2 (alternative) polarization. We examined the roles of Gαi1 and Gαi3 polarization using multiple genetic methods. Methods Results: In MEFs primary murine BMDMs, Gαi1/3 shRNA, knockout or dominant negative mutations attenuated IL-4-induced IL4Rα endocytosis, Gab1 recruitment as well activation, leaving STAT6 signaling unaffected. Following stimulation, proteins associated with intracellular domain IL-4Rα APPL1 adaptor, to mediate...
We explored the potential activity of compound 16 (Cpd16), a novel small molecule Nrf2 activator, in hydrogen peroxide (H2O2)-stimulated osteoblasts. In primary murine/human osteoblasts and MC3T3-E1 murine osteoblastic cells, Cpd16 treatment at micro-molar concentrations caused disassociation Keap1-Nrf2 cascade activation. induced stabilization protein its nuclear translocation, thereby increasing antioxidant response elements (ARE) reporter genes transcription human Significantly, mitigated...