A5102995239- Osteoarthritis Treatment and Mechanisms
- Spine and Intervertebral Disc Pathology
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Scoliosis diagnosis and treatment
- Inflammatory mediators and NSAID effects
- Bone fractures and treatments
- Orthopedic Infections and Treatments
- Spinal Fractures and Fixation Techniques
- Musculoskeletal pain and rehabilitation
- Total Knee Arthroplasty Outcomes
- Pain Mechanisms and Treatments
- Shoulder Injury and Treatment
- Periodontal Regeneration and Treatments
- Nail Diseases and Treatments
- Bone health and osteoporosis research
- Orthopedic Surgery and Rehabilitation
- Neuropeptides and Animal Physiology
- Tendon Structure and Treatment
- Hip disorders and treatments
- Antifungal resistance and susceptibility
- Knee injuries and reconstruction techniques
- Nerve injury and regeneration
- Mesenchymal stem cell research
- Cell Adhesion Molecules Research
Kitasato University
2016-2025
Shonan University of Medical Sciences
2020-2025
Nagoya University
2024
Panasonic (Japan)
2022-2024
Kitasato University Hospital
2018-2023
Aalborg University
2023
Level (Czechia)
2022
Open Evidence
2022
Chiba University Hospital
2021
Chiba University
2021
Osteoarthritis (OA) is a chronic joint disease marked by synovial inflammation, cartilage degradation, and persistent pain. Although Netrin-4 (NTN4) has been implicated in pain modulation rheumatoid arthritis (RA), its role OA remains less understood. Previous research documented that NTN4 promotes axonal growth rodent-derived neurons; however, effects on human sensory neurons are yet to be fully explored. also plays multifactorial various non-neuronal cells, such as endothelial tumor...
The object of this study was to examine the efficacy preoperative teriparatide treatment for increasing insertional torque pedicle screws during fusion surgery in postmenopausal women with osteoporosis.Fusion thoracic and/or lumbar spine performed 29 osteoporosis aged 65-82 years (mean 72.2 years). patients were divided into 2 groups based on whether they treated (n = 13) or not 16) before surgery. In teriparatide-treated group, received therapy as either a daily (20 μg/day, n 7) weekly...
Summary Nerve growth factor (NGF) functions to modulate osteoarthritis (OA)-associated pain. Although recent studies suggest that tumour necrosis (TNF)-α and interleukin (IL)-1β mediate NGF activity in human synovial fibroblasts, the regulation of expression macrophages remains unclear. Here, we examined role production (SYN) osteoarthritic knee joints by examining mRNA TNF-α IL-1β freshly isolated CD14-positive (macrophage-rich fraction) CD14-negative cells (fibroblast-rich tissue from OA...
To successfully engineer large-sized tissues, establishing vascular structures is essential for providing oxygen, nutrients, growth factors and cells to prevent necrosis at the core of tissue. The diameter scale biofabricated vasculatures should range from 100 1,000 µm support mm-size tissue while being controllably aligned spaced within diffusion limit oxygen. In this review, insights regarding biofabrication considerations techniques engineered blood vessels will be presented. Initially,...
To investigate the role of macrophages as a regulator and producer nerve growth factor (NGF) in synovial tissue (ST) osteoarthritis (OA) joints, gene expression profiles several inflammatory cytokines ST, including fibroblasts, OA mice (STR/Ort) were characterized. Specifically, real-time polymerase chain reaction analysis was used to evaluate tumor necrosis factor- (TNF-) α , interleukin- (IL-) 1 β IL-6, NGF CD11b+ CD11b– cells isolated from ST murine model. The effects TNF- IL-1 IL-6 on...
Abstract We developed a new scaffold material—oriented collagen tubes (OCT)—and evaluated the potential of OCTs combined with basic fibroblast growth factor (bFGF) to repair 15 mm sciatic nerve defect in rats. The treatment groups consisted OCT adsorbed bFGF (OCT/bFGF group), phosphate‐buffered saline (PBS) (OCT/PBS and no‐treatment group (Defect group). Functional evaluation regeneration was performed using CatWalk system, histological analyses sites were also performed. In rats treated...
Research suggests that vascular endothelial growth factor (VEGF) levels in the synovial fluid of knee osteoarthritis (KOA) patients are positively correlated with KOA severity. The relationship between VEGF and pain human is not fully understood, role pathway remains unclear. We harvested membrane (SM) from 102 radiographic evidence (unilateral Kellgren/Lawrence [K/L] grade 2–4) during total arthroplasty. Patients scored their on a 0 to 10 cm visual analog scale (VAS). SM strong/severe (VAS...
In 2009, Santoni et al. reported cortical bone trajectory (CBT) as a method of inserting pedicle screws to obtain more solid fixation, and proposed the use with closely placed thread (cortical screws) for CBT. Since entry in CBT differs from that traditional trajectory, it is unclear whether increased strength derives specific or shape screw contact bone. Whether always required thus remains unclear. The authors therefore investigated relationship between characteristics pullout animal...
ABSTRACT Nerve growth factor (NGF) is increased in intervertebral discs (IVDs) after disc injury and anti‐NGF therapy improves low back pain humans. Furthermore, M1 M2 macrophage subtypes play a role degenerative IVD injury. We examined markers NGF cytokine expression IVD‐derived cells from control IVD‐injured mice for 28 days following Ngf messenger RNA (mRNA) was 1 day injured compared with mice, persisted up to days. Flow cytometric analysis demonstrated that the proportion of F4/80+...
Macrophages, particularly M1 macrophages, produce proinflammatory cytokines and contribute to the degenerative process in injured intervertebral discs (IVDs). We previously showed that macrophages both intact IVDs increased following IVD injury. Resident recruited from peripheral blood have distinct roles tissue. However, it remains be determined whether derive resident or macrophages. investigated origin of using green fluorescent protein (GFP) transgenic bone marrow chimeric mice. The...
ABSTRACT Upregulation of inflammatory cytokines and various growth factors is a significant contributor to discogenic low back pain. The aim this study was investigate possible regulation pain‐related molecules by macrophages the role macrophage‐derived in injured intervertebral disc (IVD)s. C57BL/6J mice were used study. We characterized expression profiles genes for tumor necrosis factor (TNF)‐alpha, interleukin (IL)‐1beta, nerve (NGF), vascular endothelial (VEGF) both intact IVDs....
To clarify the roles of prolactin (PRL) and GH in control immune system, effects environmental salinity, hypophysectomy, PRL administration on several functions were examined tilapia (Oreochromis mossambicus). Transfer from fresh water (FW) to seawater (SW) did not alter plasma levels immunoglobulin M (IgM) lysozyme. The superoxide anion (O(2)(-)) production head kidney leucocytes accompanied by phagocytosis was elevated SW-acclimated fish over observed FW fish. Hypophysectomy resulted a...
Dermatophytosis is the most common skin infection caused by dermatophytic fungi, such as Trichophyton spp. We studied in vitro and vivo antifungal effects of lactoferrin against Trichophyton. Human bovine lactoferrin, a lactoferrin-derived peptide, lactoferricin B, showed activity that was dependent on test strain medium used. In guinea pigs infected back with mentagrophytes (i.e. those tinea corporis), consecutive daily po administration did not prevent development symptoms during early...
To understand more clearly the link between osteoarthritis and hyperlipidaemia, we investigated inflammatory macrophage subsets macrophage-regulated matrix metalloprotease-3 (MMP-3) A disintegrin metalloprotease with thrombospondin motifs-4 (ADAMTS4) in synovial (ST) adipose tissues (AT) of osteoarthritic mice hyperlipidaemia (STR/Ort). CD11c(+) F4/80(+) CD11b(+) populations ST AT 9-month-old STR/Ort C57BL/6J were characterized compared by flow cytometry real-time polymerase chain reaction...
Recent studies suggest that the vasodilatory neuropeptide calcitonin gene-related peptide (CGRP) is localized in synovial tissue and may be involved pathology of hip knee osteoarthritis (OA). However, regulation relationship between pain CGRP expression levels human OA patients are not fully understood.Synovial tissues were harvested from 74 participants with radiographic (unilateral Kellgren/Lawrence grades 3-4) during total arthroplasty. CGRP-expressing cells resected identified by...
Nerve growth factor (NGF) is not only an important in nerve but also a major contributor to the production of inflammation. It has been reported that inhibiting NGF could reduce several types pain animal models. Here, we aimed clarify efficacy antibody knee osteoarthritis (OA) model mice.Six-week-old male C57BR/J mice were used (n = 30). Ten comprised control group, which received saline injection into right joints; other 20 experimental monoiodoacetate (MIA) joints. Three weeks after...
Chronic inflammation with aging contributes to sarcopenia. Previous studies have suggested that the accumulation of adipose tissue in skeletal muscle, referred as intermuscular (IMAT), increases age and is associated inflammation. However, mechanism governing ectopic muscle due not fully understood. Leptin, an adipocytokine derived from tissue, important mediator inflammatory processes. We examined changes leptin levels whether To evaluate we measured alterations expression cytokine genes...
Labral tear can be the initiating factor in onset of hip osteoarthritis (HOA). However, physiopathology labral is not fully understood. Our aim was to compare synovial tissue inflammatory cytokine levels between patients with and late-stage HOA.Synovial from sites showing greatest inflammation harvested 106 hips 100 subjects during surgery. RNA extracted, TNFA, IL1B, IL6 COX2 mRNA were compared among all using real-time PCR. Additionally, we examined whether femoroacetabular impingement...
Total hip arthroplasty (THA) is widely used to treat osteoarthritis (HOA), particularly in elderly patients. However, the associated costs and complications highlight need for strategies shorten hospital stays optimise postoperative outcomes. This study aimed investigate effect of preoperative nutritional status dietary intake on discharge outcomes patients with unilateral HOA who underwent THA. A retrospective analysis 57 without significant comorbidities out 172 THA procedures performed by...