A5044846381- Parasites and Host Interactions
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Neonatal Respiratory Health Research
- Research on Leishmaniasis Studies
- Immunotherapy and Immune Responses
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Macrophage Migration Inhibitory Factor
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inflammatory mediators and NSAID effects
- IL-33, ST2, and ILC Pathways
- Cell Adhesion Molecules Research
- Tuberculosis Research and Epidemiology
- Immune cells in cancer
- vaccines and immunoinformatics approaches
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Eosinophilic Esophagitis
- Pulmonary Hypertension Research and Treatments
- Mast cells and histamine
- Mycobacterium research and diagnosis
University of Michigan
2008-2022
VA Ann Arbor Healthcare System
2009-2022
Museum of Heilongjiang Province
2021
Michigan Medicine
2004-2018
Michigan United
2010-2014
Matrix Research (United States)
2014
United States Department of Veterans Affairs
1988-2013
Cleveland Clinic
2006
A. Alfred Taubman Health Care Center
2003
Pulmonary and Critical Care Associates
2003
Monocyte chemoattractant protein-1 (MCP-1) is a potent agonist for mononuclear leukocytes and has been implicated in the pathogenesis of atherosclerosis granulomatous lung disease. To determine role MCP-1 related family members vivo, we used homologous recombination embryonic stem cells to generate mice with targeted disruption C-C chemokine receptor 2 (CCR2), MCP-1. CCR2-/- were born at expected Mendelian ratios developed normally. In response thioglycollate, recruitment peritoneal...
Cellular constituents of the alveolar-capillary wall may be key participants in recruitment polymorphonuclear leukocytes to lung through generation novel neutrophil chemotactic peptide interleukin-8 (IL-8). This interaction appears occur via ability human alveolar macrophage (AM)-derived monokines, tumor necrosis factor (TNF), and interleukin-1 (IL-1) induce gene expression IL-8 from pulmonary type II-like epithelial cells (A549). Northern blot analysis demonstrated that steady-state mRNA...
The origin of fibroblasts in pulmonary fibrosis is assumed to be intrapulmonary, but their extrapulmonary and especially derivation from bone marrow (BM) progenitor cells has not been ruled out. To examine this possibility directly, adult mice were durably engrafted with BM isolated transgenic expressing enhanced GFP. Induction such chimera by endotracheal bleomycin (BLM) injection caused large numbers GFP(+) appear active fibrotic lesions, while only a few could identified control lungs....
The origin of fibroblasts in pulmonary fibrosis is assumed to be intrapulmonary, but their extrapulmonary and especially derivation from bone marrow (BM) progenitor cells has not been ruled out. To examine this possibility directly, adult mice were durably engrafted with BM isolated transgenic expressing enhanced GFP. Induction such chimera by endotracheal bleomycin (BLM) injection caused large numbers GFP+ appear active fibrotic lesions, while only a few could identified control lungs....
Abstract We examined the role of cyclooxygenase (CO)-derived metabolites arachidonic acid (AA) in regulation interleukin 1 (IL 1) production by lipopolysaccharide (LPS)-stimulated murine resident peritoneal macrophages. The use LPS proved to be an efficacious probe, because it stimulated both IL and AA metabolism via only CO pathway. prostaglandin E2 (PGE2) I2 (PGI2; measured as its stable metabolite 6-Keto F1 alpha) LPS-stimulated macrophages was demonstrated high pressure liquid...
Human herpesvirus 8 (HHV8, also known as Kaposi's sarcoma [KS]-associated herpesvirus) has been implicated an etiologic agent for KS, angiogenic tumor composed of endothelial, inflammatory, and spindle cells. Here, we report that transgenic mice expressing the HHV8-encoded chemokine receptor (viral G protein-coupled receptor) within hematopoietic cells develop angioproliferative lesions in multiple organs morphologically resemble KS lesions. These are characterized by a spectrum changes...
Airway inflammation is characterized by leukocyte extravasation around the peribronchial mucosa and into airway of lung. In present study we utilized a model induced intratracheal challenge with soluble parasite (Schistosoma mansoni) egg Ag (SEA) in presensitized mice. The subsequent inflammatory response recruitment consists early neutrophil (8 to 24 h) later eosinophil (48 72 infiltration interstitium airway. Little no was observed control mice challenged vehicle. Multiple studies have...
Evidence for the extraneuronal accumulation of norepinephrine has been demonstrated to occur in macrophage (M phi), yet physiologic role this system remains undefined. We have assessed response murine peritoneal M phi adrenergic antagonists. also defined a phi-associated pool nonspecific agonist norepinephrine. investigated constitutive involvement alpha-adrenergic and beta-adrenergic receptors LPS-induced TNF-alpha production. CFA-elicited phis were incubated with LPS (1 microgram/ml)...
The present study examined the contribution of monocyte chemotactic protein-1 (MCP-1) to granulomatous inflammation mediated by Th1- and Th2-related cytokines. Types 1 2 lung granulomas (GR) were respectively induced in presensitized CBA mice embolization beads coupled purified protein derivative Mycobacteria tuberculosis or soluble Ags derived from Schistosoma mansoni eggs. MCP-1 was spontaneously produced intact GR, isolated GR macrophages, draining lymph node cultures, but levels greater...
Abstract Peripheral blood monocytes are important mediators of inflammation via the generation various bioactive substances, including recently isolated and cloned chemotactic peptide IL-8. Through cytokine networking, monocyte-derived cytokines capable inducing IL-8 expression from non-immune cells. IL-4, a B T lymphocyte stimulatory factor, has been shown to inhibit monocyte/macrophage function, ability suppress monocyte-generated cytokines. We describe in vitro inhibition gene synthesis...
Abstract Beginning at the time of insulitis, nonobese diabetic (NOD) mice demonstrate a thymocyte and peripheral T cell proliferative hyporesponsiveness induced by TCR cross-linking, which is associated with reduced IL-2 IL-4 secretion. We previously reported that NOD CD4+ reversed completely in vitro exogenous IL-4, administration to prevents onset insulin-dependent diabetes mellitus (IDDM). This result suggested cell-mediated destruction pancreatic islet beta cells may from regulatory Th2...
Chemokine receptors transduce signals important for the function and trafficking of leukocytes. Recently, it has been shown that CC chemokine receptor (CCR)8 is selectively expressed by Th2 subsets, but its functional relevance unclear. To address biological role CCR8, we generated CCR8 deficient (−/−) mice. Here report defective T helper type 2 (Th2) immune responses in vivo CCR8−/− mice models Schistosoma mansoni soluble egg antigen (SEA)-induced granuloma formation as well ovalbumin...
Malaria infections during pregnancy can lead to the delivery of low-birth-weight infants. In this study, cytokine mRNA was measured in placentas from 23 malaria-infected and 21 uninfected primigravid women who had delivered Mangochi, Malawi, a region with high rate transmission falciparum malaria. Significantly increased expression interleukin (IL)—1β, IL-8, tumor necrosis factor (TNF)—α decreased IL-6 transforming growth factor-β1 were found compared placentas. TNF-α IL-8 produced by...
The alveolar macrophage (AMO) in its pivotal position for pulmonary host defense may play a prominent role the orchestration of polymorphonuclear leukocyte (PMN) diapedesis. We demonstrate that hu- man AMO participate these inflammatory events through production novel neutrophil chemotactic factor, interleukin-8 (IL-8). induction AMO-derived IL-8 by tumor necrosis factor (TNF), lipopolysaccharide (LPS), and interleukin-1 (IL-1β) was shown to be both dose time depen- dent. Maximal gene...
We have examined the role of intrapulmonary TNF in a rat model acute immune complex-triggered alveolitis. Intratracheal instillation IgG anti-bovine serum albumin (anti-BSA) followed by intravenous infusion BSA results Over 4-h course evolving lung injury, 10-fold increase activity occurred bronchoalveolar lavage (BAL) fluid. Immunohistochemical analysis sections and BAL cells revealed that alveolar macrophages are chief source TNF. Antibodies specifically neutralize were raised rabbits...
Abstract The number of memory phenotype CD8 T cells increases dramatically with aging in both humans and mice. However, the mechanism for this is unknown. prevailing hypothesis that accumulate as a result lifelong antigenic stimulation. data supporting supposition are lacking. In study, we demonstrate central cells, which represent large majority aged mice, not develop response to stimulation but virtual without addition phenotypic evidence, show accumulation independent CD4 CCR5, CXCR3, all...
Abstract Allergic airway inflammation is characterized by peribronchial leukocyte accumulation within the airway. Subsequent tissue damage leading to hyperreactivity a result of activation multiple populations. Using an established model allergic induced intratracheal challenge with parasite (Schistosoma mansoni) egg Ag in presensitized mice, we have examined differential recruitment. These studies identified key chemokines involved specific subsets cells and induction hyperreactivity. In...
The establishment of animal models asthma is critical to elucidate the mechanisms involved in pathogenesis disease. In present study, we have used a parasite antigen from Schistosoma mansoni eggs, which induces TH2 response, elicit pulmonary inflammatory reaction that resolves after 3 4 days. Histologic examination lungs soluble egg antigens (SEA) or saline vehicle-challenged mice demonstrated large influx cells antigen- but not mice, thus demonstrating an antigen-specific reaction. A...
Abstract Malaria during pregnancy is associated with poor birth outcomes, particularly low weight. Recently, monocyte infiltration into the placental intervillous space has been identified as a key risk factor for However, malaria-induced chemokines involved in recruiting and activating monocytes have not identified. In this study, we determined which are elevated malaria infection association between chemokine expression infiltration. Placental was elevations mRNA of three β chemokines,...
The pulmonary fibroblast's (PF) unique location allows it to communicate in a bidirectional fashion between the vascular compartment and alveolar airspace, placing strategic position for elicitation of inflammatory leukocytes into lung. In this study, we demonstrate that PF may contribute inflammation through production potent neutrophil chemotactic factor, interleukin (IL)-8. PF-derived IL-8 expression was dependent upon stimulation by either tumor necrosis factor (TNF) or IL-1 but not...