A5100744393- Probabilistic and Robust Engineering Design
- Probiotics and Fermented Foods
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Structural Health Monitoring Techniques
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- stochastic dynamics and bifurcation
- Meat and Animal Product Quality
- Wind and Air Flow Studies
- Microbial Natural Products and Biosynthesis
- Acoustic Wave Phenomena Research
- Statistical Mechanics and Entropy
- Insect Utilization and Effects
- Plant biochemistry and biosynthesis
- Statistical Distribution Estimation and Applications
- Enzyme Catalysis and Immobilization
- Ship Hydrodynamics and Maneuverability
- Vibration and Dynamic Analysis
- Polysaccharides and Plant Cell Walls
- Electrocatalysts for Energy Conversion
- Microbial Community Ecology and Physiology
- Fungal Biology and Applications
Tianjin University
2016-2025
Shaoxing University
2025
The First Affiliated Hospital, Sun Yat-sen University
2024
Sun Yat-sen University
2024
University of Science and Technology of China
2024
Shandong Institute of Automation
2022
Chinese Academy of Sciences
2022
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2015-2019
John Wiley & Sons (United Kingdom)
2018-2019
Hudson Institute
2018-2019
Learning high-quality sentence representations is a fundamental problem of natural language processing which could benefit wide range downstream tasks. Though the BERT-like pre-trained models have achieved great success, using their directly often results in poor performance on semantic textual similarity task. Recently, several contrastive learning methods been proposed for and shown promising results. However, most them focus constitution positive negative representation pairs pay little...
Abstract Background Photosynthetic cyanobacteria have been recently proposed as a ‘microbial factory’ to produce butanol due their capability utilize solar energy and CO 2 the sole carbon sources, respectively. However, improve productivity, one key issue needed be addressed is low tolerance of photosynthetic hosts butanol. Results In this study, we first applied quantitative transcriptomics approach with next-generation RNA sequencing technology identify gene targets relevant in model...
α-Farnesene, a type of acyclic sesquiterpene, is an important raw material in agriculture, aircraft fuel, and the chemical industry. In this study, we constructed efficient α-farnesene-producing yeast cell factory by combining enzyme metabolic engineering strategies. First, screened different plants for α-farnesene synthase (AFS) with best activity found that AFS from Camellia sinensis (CsAFS) exhibited most production Saccharomyces cerevisiae 4741. Second, flux mevalonate pathway was...
ABSTRACT To overcome the adverse impacts of environmental stresses during growth, different adaptive regulation mechanisms can be activated in Lactococcus lactis . In this study, transcription levels eight transcriptional regulators L. subsp. F44 under acid stress were analyzed using quantitative reverse transcription-PCR. Eight gene-overexpressing strains then constructed to examine their influences on acid-resistant capability. Overexpressing ythA , a PspC family regulator, increased...
L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose been achieved Escherichia coli. However, there are still several limiting factors hindering further improvement productivity, such as the conflict between cell growth production, byproduct accumulation, insufficient availability cofactors (adenosine triphosphate, NADH, NADPH). Here, metabolic modification strategy...
Nisin, one kind of natural antimicrobial peptide, is produced by certain Lactococcus lactis strains, which generally require expensive high-quality nitrogen sources due to limited ability amino acids biosynthesis. Here we use defatted soybean meal (DSM) as sole source support L. growth and nisin production. DSM medium composition fermentation conditions were optimized using the methods Plackett-Burman design central composite design. The highest production 3879.58 IU/ml was obtained in...
Lactococcus lactis, widely used in the manufacture of dairy products, encounters various environmental stresses both natural habitats and during industrial processes. It has evolved intricate machinery stress sensing defense to survive harsh conditions. Here, we identified a novel TetR/AcrR family transcription regulator, designated AcrR1, be repressor for acid antibiotic tolerance that was derepressed presence vancomycin or under stress. The survival rates acrR1 deletion strain ΔAcrR1 were...
Microbiological contamination and oxidative damage are the two main challenges in maintaining quality improving shelf-life of foods. Here, we developed a Lactococcus lactis fermentation system that could simultaneously produce nisin, an antimicrobial peptide, γ-aminobutyric acid (GABA), antioxidant agent. In this system, metabolically engineered nisin producing strain L. F44 for GABA production by expression glutamate decarboxylase glutamate/GABA antiporter. biosynthesis facilitate through...