A5014838811- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Microbial Natural Products and Biosynthesis
- Probiotics and Fermented Foods
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Natural Compounds in Disease Treatment
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Biopolymer Synthesis and Applications
- Analytical chemistry methods development
- Laser-induced spectroscopy and plasma
- Bacterial biofilms and quorum sensing
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolism and Applications
- Soybean genetics and cultivation
- Antimicrobial Peptides and Activities
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
Tianjin University
2014-2025
Shaoxing University
2024-2025
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2018-2023
Ministry of Education of the People's Republic of China
2016-2022
John Wiley & Sons (United States)
2018
Hudson Institute
2018
Nankai University
2007
Microbial communities are shaped by the complex interactions among organisms and environment. Genome-scale metabolic models (GEMs) can provide deeper insights into complexity ecological properties of various microbial communities, revealing their intricate interactions. Many researchers have modified GEMs for based on specific needs. Thus, need to be comprehensively summarized better understand trends in development. In this review, we key developments deciphering designing using different...
Abstract Traditionally, nisin was produced industrially by using Lactococcus lactis in the neutral fermentation process. However, showed higher activity acidic environment. How to balance pH value for bacterial normal growth and might be key problem. In this study, 17 acid-tolerant genes 6 lactic acid synthetic were introduced L. F44, respectively. Comparing 2810 IU/mL yield of original strain titer engineered strains over-expressing hdeAB , ldh murG increased 3850, 3979 4377 IU/mL, These...
α-Farnesene, a type of acyclic sesquiterpene, is an important raw material in agriculture, aircraft fuel, and the chemical industry. In this study, we constructed efficient α-farnesene-producing yeast cell factory by combining enzyme metabolic engineering strategies. First, screened different plants for α-farnesene synthase (AFS) with best activity found that AFS from Camellia sinensis (CsAFS) exhibited most production Saccharomyces cerevisiae 4741. Second, flux mevalonate pathway was...
L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose been achieved Escherichia coli. However, there are still several limiting factors hindering further improvement productivity, such as the conflict between cell growth production, byproduct accumulation, insufficient availability cofactors (adenosine triphosphate, NADH, NADPH). Here, metabolic modification strategy...
Celastrus angulatus Maxim is a kind of crucial and traditional insecticidal plant widely distributed in the mountains southwest China. Celangulin V efficient sesquiterpenoid C. used pest control China, but low yield discontinuous supply impeded its further popularization application. Fortunately, development synthetic biology provided an opportunity for sustainable V, which understanding biosynthetic pathway indispensable. In this study, six cDNA libraries were prepared from leaf root before...
Due to the barriers imposed by restriction-modification (RM) system, Nisin-producing industrial strains of Lactococcus lactis often encounter low transformation efficiency, which seriously hinders widespread application genetic engineering in non-model L. lactis. Herein, we present a novel pre-modification strategy (PMS) coupled with optimized plasmid delivery systems designed systematically evade RM and substantially improve Nisin biosynthesis Through use engineered Escherichia coli...
Food-derived compounds represent a promising reservoir for developing novel therapeutic agents against pathogenic infections such as Salmonella Typhimurium. In this study, we integrated computational biology with experimental validation to identify...
Low-energy laser has been widely applied in breeding of microorganisms, treatment wound healing, and cancer therapy for many years, but the molecular mechanisms irradiation are still unclear. In this study, biological effects He-Ne on dibenzothiophene monooxygenase ( DszC ) were analyzed, comparing wild-type bacteria strain Gordonia sp. WQ-01 its irradiated WQ-01A. First, gene protein sequences analyzed. The results indicated that there ten point mutations dszC sequences, four them further...
As d -amino acids play an important role in cell wall synthesis, we analyzed the effects of 19 different on L. lactis F44, demonstrating that -Met and -Phe can participate peptidoglycan (PG) synthesis improve acid resistance nisin yield this strain. murF overexpression further increased levels incorporated into PG contributed to These findings suggest be , study provides new ideas for enhancement production.
Volatile terpenoids are a large group of important secondary metabolites and possess many biological activities. The acyclic sesquiterpene trans-nerolidol is one the typical representatives widely used in cosmetics agriculture. Here, accumulation volatile terpenes different tissues Celastrus angulatus was investigated, two synthases, CaNES1 CaNES2, were identified characterized by vitro enzymatic assays. Both genes differentially transcribed C. angulatus. Next, we constructed Saccharomyces...
Protopanaxadiol (PPD), an active triterpene compound, is the precursor of high-value ginsenosides. In this study, we report a strategy for enhancement PPD production in Saccharomyces cerevisiae by cofermentation glucose and xylose. mixed sugar fermentation, strain GW6 showed higher titer yield than that obtained from cultivation. Then, engineering strategies were implemented on to enhance yields, such as adjustment central carbon metabolism, optimization mevalonate pathway, reinforcement...
Microbiological contamination and oxidative damage are the two main challenges in maintaining quality improving shelf-life of foods. Here, we developed a Lactococcus lactis fermentation system that could simultaneously produce nisin, an antimicrobial peptide, γ-aminobutyric acid (GABA), antioxidant agent. In this system, metabolically engineered nisin producing strain L. F44 for GABA production by expression glutamate decarboxylase glutamate/GABA antiporter. biosynthesis facilitate through...