A5081867087- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Enzyme Catalysis and Immobilization
- Aquaculture disease management and microbiota
- Biochemical and Molecular Research
- Aquaculture Nutrition and Growth
- Microbial Natural Products and Biosynthesis
- Fermentation and Sensory Analysis
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
- Antibiotic Resistance in Bacteria
- Mycotoxins in Agriculture and Food
- Microbial infections and disease research
- Protist diversity and phylogeny
- Bacteriophages and microbial interactions
- Plant biochemistry and biosynthesis
- Oral microbiology and periodontitis research
- Bacterial Genetics and Biotechnology
- Reproductive biology and impacts on aquatic species
- Viral gastroenteritis research and epidemiology
- Glycosylation and Glycoproteins Research
- Biochemical and biochemical processes
Nankai University
2016-2025
Northeast Forestry University
2025
Zhuzhou Central Hospital
2025
Shandong Academy of Sciences
2021-2024
Qilu University of Technology
2021-2024
Ministry of Education of the People's Republic of China
2015-2024
Third Affiliated Hospital of Zhengzhou University
2024
The University of Queensland
2023
Chinese Academy of Sciences
2023
Institute of Computing Technology
2023
Abstract Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S . exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on synthesis are mediated bacterial protein SopE2, a type III secretion system (T3SS) effector encoded pathogenicity island SPI-1. changes metabolism promote intracellular replication via two mechanisms: glucose levels...
Case-Based Learning (CBL) and Problem-Based (PBL) are popular methods in medical education. However, we do not fully understand how they affect the clinical thinking skills of Assistant General Practitioner (AGP) trainees. This randomised controlled trial aimed to assess effectiveness combining CBL PBL compare their impact on AGP trainees with that traditional lecture-based learning (LBL). involved 70 second-year who were randomly assigned either CBL-PBL group or LBL using a simple...
Abstract Monascus has been used to produce natural colorants and food supplements for more than one thousand years approximately billion people eat Monascus- fermented products during their daily life. In this study, using next-generation sequencing optical mapping approaches, a 24.1-Mb complete genome of an industrial strain, purpureus YY-1, was obtained. This consists eight chromosomes 7,491 genes. Phylogenetic analysis at the level provides convincing evidence evolutionary position M. ....
Abstract A feeding trial was conducted to evaluate dietary protein and lipid requirements for juvenile largemouth bass, Micropterus salmoides . 4 × 2‐factorial layout included four (420, 450, 480, 510 g/kg) two (80 120 levels. Fish (initial weight 8.7 g) were fed the test diets 8 wk. Weight gain, feed intake, conversion ratio, hepatosomatic index, body composition dependent on level. Nitrogen retention efficiency independent of level, their interaction. gain higher in fish diet containing...
A series of multiaxial low‐cycle fatigue experiments was performed on 45 steel under non‐proportional loading. The present evaluations life were systematically analysed. combined energy density and critical plane concept is proposed that considers different failure mechanisms for a shear‐type tensile‐type failure, from which damage parameters the plane‐strain are proposed. For failures in material 42CrMo steel, new permit good prediction currently used models special simple form model.
ABSTRACT FK506, a widely used immunosuppressant, is produced by industrial fermentation processes using various Streptomyces species. However, the low titer becomes bottleneck for its application and industrialization. It urgently required full understanding of biological mechanisms FK506 overproduction. Towards this end, comparative metabolomics approach was employed to analyze metabolite concentrations difference tsukubaensis cultivated in two media with high productivities. Initially, 98...
Abstract Background FK506 is an important immunosuppressant, which can be produced by Streptomyces tsukubaensis . However, the production capacity of strain very low. Hereby, a computational guided engineering approach was proposed in order to improve intracellular precursor and cofactor availability S. Results First, genome-scale metabolic model constructed based on its annotated genome biochemical information. Subsequently, several potential genetic targets (knockout or overexpression)...
Chrysanthemum morifolium, one of the most economically important ornamental crops worldwide, is well-known for elaborate and complex inflorescence which composed both bilaterally symmetrical ray florets radially disc florets. Despite continuing efforts, molecular mechanisms underlying regulation two flower types are still unclear so far. CYC-like proteins have been shown to control symmetry or regulate flower-type identity in several angiosperm plant lineages. In this study, we conducted...
Abstract Background Isobutanol is considered as a leading candidate for the replacement of current fossil fuels, and expected to be produced biotechnologically. Owing valuable features, Bacillus subtilis has been engineered an isobutanol producer, whereas it needs further optimized more efficient production. Since elementary mode analysis (EMA) powerful tool systematical metabolic network structures cell metabolism, might great importance in rational strain improvement. Results Metabolic...
Clostridium acetobutylicum is a model fermentative anaerobe for consolidated bioprocessing of lignocellulose hydrolysates into acetone-butanol-ethanol (ABE). However, the main inhibitors (acids, furans and phenols) ubiquitous in strictly limit conversion efficiency. Thus, it essential to understand underlying mechanisms hydrolysate identify key industrial bottlenecks that undermine efficient biofuel production. The recently developed omics strategy intracellular metabolites protein...
Abstract Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of cascades, thereby achieving effective biosynthetic pathways. This paper reviews recent progress design strategy and application multi-enzyme complexes. First, metabolic channels complex introduced, construction strategies emerging years summarized. Then, discovered cascades related discussed, emphasizing...
Escherichia coli are part of the normal intestinal microbiome, but some enterohemorrhagic E. (EHEC) and enteropathogenic (EPEC) strains can cause potentially life-threatening gastroenteritis. Virulence factors underlying ability EHEC EPEC to disease include those encoded in locus enterocyte effacement (LEE) pathogenicity island. Here, we demonstrated that EsrL, a small RNA present many strains, promoted pathogenicity, adhesion, biofilm formation EPEC. PhoB, response regulator two-component...
Abstract A feeding trial was conducted to evaluate the potential of replacing fishmeal with poultry byproduct meal ( PBM ) and soybean in diets for largemouth bass, Micropterus salmoides . reference diet (C) contained 400 g/kg fishmeal, 40 or 60% replaced a blend pet‐food‐grade (diets PP1 PP2 feed‐grade PF1 PF2 ). No significant differences were found weight gain, nitrogen retention efficiency NRE ), condition factor, body composition among fish fed , Feed intake feed conversion ratio FCR...
Enterohemorrhagic Escherichia coli serotype O157:H7 (O157) is a critical, foodborne, human intestinal pathogen that causes severe acute hemorrhagic diarrhea, abdominal cramping, and even death. Small RNAs (sRNAs) are noncoding regulatory molecules sense environmental changes trigger various virulence-related signaling pathways; however, few such sRNAs have been identified in O157. Here, we report novel sRNA, EsrF senses high ammonium concentrations the colon enhances O157 pathogenicity by...