A5078628644- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Banana Cultivation and Research
- Genomics and Phylogenetic Studies
- Research in Social Sciences
- Bacteriophages and microbial interactions
- Potato Plant Research
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Resistance
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Microbial Metabolism and Applications
- Plant pathogens and resistance mechanisms
- Plant Molecular Biology Research
- Vibrio bacteria research studies
- Entomopathogenic Microorganisms in Pest Control
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Mycobacterium research and diagnosis
- Plant Stress Responses and Tolerance
- Insect symbiosis and bacterial influences
University of Helsinki
2014-2024
Finnish Food Authority
2018
Natural Resources Institute Finland
2018
Tieto (Finland)
2018
Plant Production Research Institute
2000-2009
Swedish University of Agricultural Sciences
1987-2005
TCL (China)
2004
GTx (United States)
1999-2004
University of Maryland, College Park
1996-1997
Valio (Finland)
1986
Dickeya species (formerly Erwinia chrysanthemi ) cause diseases on numerous crop and ornamental plants world‐wide. spp. (probably D. dianthicola were first reported potato in the Netherlands 1970s have since been detected many other European countries. However, 2004–5 a new pathogen, with proposed name ‘ solani ’, has spreading across Europe via trade seed tubers is causing increasing economic losses. Although disease symptoms are often indistinguishable from those of more established...
Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on basis production PCR product with pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase phosphatases ability produce indole acids...
Many plant pathogens secrete toxins that enhance microbial virulence by killing host cells. Usually, these are produced particular taxa, such as bacteria or fungi. In contrast, many bacterial, fungal and oomycete species produce necrosis ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) trigger leaf immunity-associated responses in various plants. We have determined the crystal structure of an NLP from phytopathogenic Pythium aphanidermatum to 1.35A resolution. The protein fold...
Soft rot disease is economically one of the most devastating bacterial diseases affecting plants worldwide. In this study, we present novel insights into phylogeny and virulence soft model Pectobacterium sp. SCC3193, which was isolated from a diseased potato stem in Finland early 1980s. Genomic approaches, including proteome genome comparisons all sequenced bacteria, revealed that previously included species carotovorum, can now be more accurately classified as wasabiae. Together with...
Pectinolytic Gram-negative bacteria were isolated from different waterways in the UK and Finland. Three strains (174/2 T , 181/2 Dw054) had same 16S rRNA gene sequences which shared 99 % sequence similarity to species of genus Dickeya a phylogeny related genera confirmed attribution this genus. Fatty acid profile analysis all three found high proportion C 16 : 1 ω7 c /C 0 fatty acids, library searches closest matches chrysanthemi . Production concatenated using six loci, recA gapA atpD gyrB...
Oligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact long OGs (degree polymerization (DP) from 10–15), demonstrating induction plant defense resulting enhanced defenses to necrotrophic pathogens. To clarify role trimers (trimeric OGs, DP3) DAMP their regulation, we performed a transcriptomic analysis through RNA sequencing Arabidopsis thaliana exposed...
Erwinia carotovora subsp. is a gram-negative bacterium that causes soft rot disease of many cultivated crops. When collection E. isolates was analyzed on Southern blot using the harpin-encoding gene hrpN as probe, several harpinless were found. Regulation virulence determinants in one these, strain SCC3193, has been characterized extensively. It fully virulent potato and Arabidopsis thaliana. An RpoS (SigmaS) mutant producing elevated levels secreted proteins, found to cause lesions...
Abstract Pectobacterium atrosepticum is a Gram‐negative plant pathogenic bacterium that causes rotting in potato stems and tubers. The secreted proteins of this pathogen were analyzed with proteomics from culture supernatant cells grown minimal medium supplemented host extracts. More than 40 identified, among them known virulence determinants, such as pectic enzymes, metalloprotease, protein Svx, along flagella proteins, GroEL cyclophilin‐type chaperones several hypothetical or unknown...
ABSTRACT When analyzing the secretome of plant pathogen Pseudomonas syringae pv. tomato DC3000, we identified hemolysin-coregulated protein (Hcp) as one secreted proteins. Hcp is assumed to be an extracellular component type VI secretion system (T6SS). Two copies hcp genes are present in P. DC3000 genome, hcp1 (PSPTO_2539) and hcp2 (PSPTO_5435). We studied expression patterns tested fitness knockout mutants host colonization intermicrobial competition. found that gene expressed most actively...
'Candidatus Liberibacter solanacearum' (CLso) haplotype C is associated with disease in carrots and transmitted by the carrot psyllid Trioza apicalis. To identify possible other sources vectors of this pathogen Finland, samples were taken wild plants within near fields, psyllids feeding on these plants, parsnips growing next to carrots, seeds. For analyzing genotype CLso-positive samples, a multilocus sequence typing (MLST) scheme was developed. CLso detected 11% T. anthrisci 35% Anthriscus...
Pectobacterium strains isolated from potato stems in Finland, Poland and the Netherlands were subjected to polyphasic analyses characterize their genomic phenotypic features. Phylogenetic analysis based on 382 core proteins showed that isolates clustered closest polaris but could be divided into two clades. Average nucleotide identity (ANI) revealed one of clades included P. type strain, whereas second clade was at border species with a 96 % ANI value. In silico genome-to-genome comparisons...
The production of the main virulence determinants plant pathogen Erwinia carotovora subsp. carotovora, extracellular cell wall-degrading enzymes, is partly controlled by diffusible signal molecule N-(3-oxohexanoyl)-l-homoserine lactone (OHHL). OHHL synthesized product expI/carI gene. Linked to expI we found a gene encoding putative transcriptional regulator LuxR-family. This gene, expR Ecc , transcribed convergently and two open reading frames are partially overlapping. ExpR protein showed...
Production of extracellular, plant cell wall degrading enzymes, the main virulence determinants pathogen Erwinia carotovora subsp. carotovora, is coordinately controlled by a complex regulatory network. Insertion mutants in exp (extracellular enzyme production) loci exhibit pleiotropic defects and growth-phase-dependent transcriptional activation genes encoding extracellular enzymes. Two new mutations, designated expA expS, were characterized. Introduction corresponding wild-type alleles to...
The specific recognition of elicitors produced by plant pathogenic bacteria carrying avirulence (avr) genes is postulated to initiate cellular defense responses in plants expressing corresponding resistance genes. biochemical functions most avr genes, however, are not known. A heterologous system was developed phenotypically express Pseudomonas syringae Escherichia coli cells that required the P. hrp cluster. E. MC4100 transformants plasmic-borne pv. Pss61 cluster and p. glycinea avrB...
Carrot psyllid T rioza apicalis was recently found to carry the plant pathogenic bacterium ‘ C andidatus L iberibacter solanacearum’ ( CL s). To confirm transmission of bacteria by psyllids and dissect symptoms caused in carrot plants feeding s infection, a greenhouse experiment with single on separate performed. A positive correlation between amount corresponding exposed feeding, indicating transmission. The female more severe damage than male resulted substantial decrease root weight....
Pectobacterium atrosepticum is a Gram-negative plant-pathogenic bacterium that rots potato stems and tubers. Microarray analysis was used to identify genes were differentially expressed when host extracts added the growth medium. Potato downregulated expression of ribosomal related uptake metabolism nutrients, upregulated needed for nitrate or phosphonate use. Some observed changes in gene host-extract-induced cultures are similar those during attachment tissues. Other responses indicated...