A5065230837- Plant-Microbe Interactions and Immunity
- Identification and Quantification in Food
- Plant Pathogenic Bacteria Studies
- Spectroscopy and Chemometric Analyses
- Mosquito-borne diseases and control
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Genomics and Phylogenetic Studies
- Advanced Chemical Sensor Technologies
- Legume Nitrogen Fixing Symbiosis
- Listeria monocytogenes in Food Safety
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Biomedical Ethics and Regulation
- Saffron Plant Research Studies
- Insect symbiosis and bacterial influences
- Genetic diversity and population structure
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Vibrio bacteria research studies
- Banana Cultivation and Research
- Plant Disease Resistance and Genetics
- Artificial Intelligence in Healthcare and Education
- Environmental DNA in Biodiversity Studies
European Commission
2020-2024
Sciensano (Belgium)
2018-2019
University of Helsinki
2013
Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on basis production PCR product with pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase phosphatases ability produce indole acids...
Soft rot Enterobacteria in the genera Pectobacterium and Dickeya cause rotting of many crop plants. A new isolate has been suggested to form a separate species, given name solani. This bacterium is spreading fast replacing closely related, but less virulent, potato pathogens. The genome D. solani D s0432-1 shows highest similarity at nucleotide level synteny dadantii strain 3937, it also contains three large polyketide/fatty acid/non-ribosomal peptide synthetase clusters that are not present...
Consumers and companies associated with food or pharmaceuticals rely on spices herbs in various forms. Their intricate supply chains, elevated prices, low-volume production render them vulnerable to fraudulent practices. However, comprehensive methodologies detect adulterants remain scarce, impeding national control laboratories from enforcing European legislation. In this study, we present quantitative real-time PCR (qPCR) methods designed identify the top five of each six commonly consumed...
Viral infection by dengue virus is a major public health problem in tropical countries. Early diagnosis and detection are increasingly based on quantitative reverse transcriptase real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result mismatches of primers probes with their targeted nucleic acid regions. Whole genome sequencing allows to characterize track such changes, which turn enables...
The re-emergence and spread of tropical viruses to new areas has raised a wave concern worldwide. In order treat patients at an early stage prevent the diffusion outbreak, diagnosis, therefore fast adequate detection, is needed. To this end, multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed detect Zika (ZIKV) chikungunya (CHIKV) simultaneously.Two methods targeting different genome segments were selected from literature for each virus. These...
DNA technology for food authentication is already well established, and with the advent of Next Generation Sequencing (NGS) and, more specifically, metabarcoding, compositional analysis at molecular level has rapidly gained popularity. This led to several reports in media about presence foreign, non-declared species commodities. As herbs spices are attractive targets fraudulent manipulation, a combination digital PCR metabarcoding by NGS was employed check purity 285 oregano samples taken...
<ns3:p>The <ns3:italic>JRC COVID-19 In Vitro Diagnostic Devices and Test Methods Database</ns3:italic>, aimed to collect in a single place all publicly available information on performance of CE-marked <ns3:italic>in vitro</ns3:italic> diagnostic medical devices (IVDs) as well house</ns3:italic> laboratory-developed related test methods for COVID-19, is here presented. The database, manually curated regularly updated, has been developed follow-up the Communication from European Commission...
The EU General Food Law not only aims at ensuring food safety but also to 'prevent fraudulent or deceptive practices; the adulteration of food; and any other practices which may mislead consumer'. Especially partial complete, deliberate, intentional substitution valuable ingredients (e.g., Saffron) for less ones is concern. Due variety products on market an approach detect that works well one species be easily applicable another. Here we present a broadly detection biological materials based...
Spices and herbs are used in multiple forms by consumers companies related to food or pharmaceuticals. Their intricate supply chains, high prices limited productions make them susceptible deceptive practises. However, robust methodology detect adulterants is still rarely available, preventing national control laboratories from enforcing European regulations. This article presents qPCR methods developed identify six commonly consumed spices herbs, namely paprika, turmeric, saffron, cumin,...