A5075056690- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Ocular Surface and Contact Lens
- Sleep and Wakefulness Research
- Neuroendocrine regulation and behavior
- Pharmacological Receptor Mechanisms and Effects
- Stress Responses and Cortisol
- Heart Rate Variability and Autonomic Control
- Neurotransmitter Receptor Influence on Behavior
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Glaucoma and retinal disorders
- Biochemical effects in animals
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Cannabis and Cannabinoid Research
- Cellular transport and secretion
- Corneal surgery and disorders
- Ion Channels and Receptors
- Gastroesophageal reflux and treatments
- Intraocular Surgery and Lenses
Oregon Health & Science University
2016-2025
Washington State University Vancouver
2009
Ludwig-Maximilians-Universität München
2002-2004
ZRT Laboratory
2004
University of Virginia
2002
Cornell University
1991-2001
New York University
1996
University of Iowa
1988-1991
The analgesic effects of morphine are mediated, in part, by periaqueductal gray (PAG) neurons that project to the rostral ventromedial medulla (RVM). Although much neural circuitry within RVM has been described, relationship between and PAG input spinal output is not known. objective this study was determine whether GABAergic from target reticulospinal neurons. Immunocytochemistry confocal microscopy revealed extensively projecting cord, two-thirds these appear be (contain GAD67...
Kisspeptin (Kiss1) neurons are essential for reproduction, but their role in the control of energy balance and other homeostatic functions remains unclear. Proopiomelanocortin (POMC) agouti-related peptide (AgRP) neurons, located arcuate nucleus (ARC) hypothalamus, integrate numerous excitatory inhibitory inputs to ultimately regulate homeostasis. Given that POMC AgRP contacted by Kiss1 ARC (Kiss1(ARC)) they express androgen receptors, Kiss1(ARC) may mediate orexigenic action testosterone...
Chronic demyelination and oligodendrocyte loss deprive neurons of crucial support. It is the degeneration their connections that drives progressive disability in demyelinating disease. However, whether chronic triggers neurodegeneration how it may do so remain unclear. We characterize two genetic mouse models inducible demyelination, one distinguished by effective remyelination other failure demyelination. While both lines feature axonal damage, mice with blocked have elevated neuronal...
The rostral ventrolateral medulla (RVL) contains reticulospinal adrenergic (C1) neurons that are thought to be sympathoexcitatory and form the medullary efferent limb of baroreceptor reflex pathway. RVL receives direct projections from two important autonomic regions, caudal (CVL) nucleus tractus solitarii (NTS). In present study, we used anterograde tracing CVL or NTS combined with immunocytochemical identification C1 in compare morphology afferent input these regions into RVL. (n = 203)...
Cranial visceral afferents activate central pathways that mediate systemic homeostatic processes. Afferent information arrives in the brainstem nucleus of solitary tract (NTS) and is relayed to other CNS sites for integration into autonomic responses complex behaviors. Little known about organization or nature processing within NTS. We injected fluorescent retrograde tracers two nuclei identify neurons project involved regulation: caudal ventrolateral medulla (CVLM) paraventricular...
N-Methyl-d-aspartate receptors are present in the nodose ganglion, which contains cell bodies of vagal afferents, and nucleus tractus solitarius, where these afferent fibers terminate. This suggests that N-methyl-d-aspartate located presynaptically on visceral afferents and/or their target neurons solitarius. To test this hypothesis, we combined anterograde transport biotinylated dextran amine, following injections into left with electron microscopic immunogold labeling antipeptide antiserum...
Although the central effects of insulin to activate sympathetic nervous system and enhance baroreflex gain are well known, specific brain site(s) at which acts has not been identified. We tested hypotheses that (1) paraventricular nucleus hypothalamus (PVN) arcuate (ArcN) necessary sites (2) initiates its directly in PVN and/or ArcN. In α-chloralose anaesthetised female Sprague–Dawley rats, mean arterial pressure (MAP), heart rate (HR) lumbar nerve activity (LSNA) were recorded continuously,...
Abstract Fear and emotional learning are modulated by endogenous opioids but the cellular basis for this is unknown. The intercalated cells (ITCs) gate amygdala output thus regulate fear response. Here we find released synaptic stimulation to act via two distinct mechanisms within main ITC cluster. Endogenously inhibit glutamate release through δ-opioid receptor (DOR), an effect potentiated a DOR-positive allosteric modulator. Postsynaptically, activate potassium conductance μ-opioid (MOR),...
<h3>Abstract</h3> We previously reported that perineuronal nets (PNNs) are required for cocaine-associated memories. Perineuronal extracellular matrix primarily surrounds parvalbumin (PV)-containing, GABAergic fast-spiking interneurons (FSIs) in the medial prefrontal cortex (mPFC). Here we measured impact of acute (1 d) or repeated (5 cocaine exposure on PNNs and PV cells within prelimbic infralimbic regions mPFC. Adult rats were exposed to 1 5 d stained (using <i>Wisteria floribunda</i>...
Junctions between the endoplasmic reticulum (ER) and plasma membrane (PM) are specialized contacts ubiquitous in eukaryotic cells. Concentration of intracellular signaling machinery near ER-PM junctions allows these domains to serve critical roles lipid Ca2+ homeostasis. Subcellular compartmentalization protein kinase A (PKA) also regulates essential cellular functions, however, no specific association PKA junctional is known. Here, we show that brain neurons type I directed Kv2.1...
Do continuous subthreshold oscillations in membrane potential within an electrically coupled network depend on gap junctional coupling? For the inferior olive (IO), modeling and developmental studies suggested that answer is yes, although physiological of connexin36 knock-out mice lacking electrical coupling no. Here we addressed question differently by using a lentivirus-based vector to express, IO adult rats, single amino acid mutation disrupts intracellular trafficking wild-type blocks...
In experiment 1, quantitative regional comparisons of the antinociceptive and cardiovascular responses produced by electrical stimulation in caudal medulla, including regions such as nucleus tractus solitarius (NTS), reticularis ventralis (NRV), gigantocellularis (NRGC), paragigantocellularis (NRPGC), raphe obscurus (NRO), medial portions lateral reticular (LRN), were made rat. Electrical all these resulted inhibition nociceptive tail-flick reflex, although threshold intensity for was...
Several chronic pain disorders, such as migraine and fibromyalgia, have an increased prevalence in the female population. The underlying mechanisms of this sex-biased yet to be thoroughly documented, but could related endogenous differences neuromodulators networks, including endocannabinoid system. cellular system comprises lipid signals 2-AG (2-arachidonoylglycerol) AEA (anandamide); enzymes that synthesize degrade them; cannabinoid receptors. relative different components specific brain...
Alpha-adrenergic receptor, subtype 2A (α2A-AR), activation is one of the primary modes action for norepinephrine (NE) in rat hippocampal formation. In this study, α2A-AR immunoreactivity (α2A-AR-I) was localized by light and electron microscopy hippocampus dentate gyrus using a previously characterized antibody to α2A-AR. By microscopy, dense α2A-AR-I observed pyramidal granule cell layers. Diffuse slightly granular found neuropil all other laminae, notably stratum lacunosum-moleculare....
Many individuals with multiple sclerosis (MS) experience clinically significant pain, yet the underlying neural mechanisms for MS pain are not understood. Experimental autoimmune encephalomyelitis (EAE) is a well-studied disease in rodents that mimics many clinical and pathological features of MS, including central nervous system inflammation demyelination. To determine whether EAE an appropriate model MS-related nociceptive responses both male female SJL mice were measured before after...